scholarly journals Domain-III FG loop of the dengue virus type 2 envelope protein is important for infection of mammalian cells and Aedes aegypti mosquitoes

Virology ◽  
2010 ◽  
Vol 406 (2) ◽  
pp. 328-335 ◽  
Author(s):  
Steven M. Erb ◽  
Siritorn Butrapet ◽  
Kelley J. Moss ◽  
Betty E. Luy ◽  
Thomas Childers ◽  
...  
PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e20528 ◽  
Author(s):  
Adriana S. Azevedo ◽  
Anna M. Y. Yamamura ◽  
Marcos S. Freire ◽  
Gisela F. Trindade ◽  
Myrna Bonaldo ◽  
...  

2014 ◽  
Vol 453 (3) ◽  
pp. 309-315 ◽  
Author(s):  
Sai Hari A. Gandham ◽  
David E. Volk ◽  
Ganesh L.R. Lokesh ◽  
Muniasamy Neerathilingam ◽  
David G. Gorenstein

2009 ◽  
Vol 5 (2) ◽  
pp. e1000299 ◽  
Author(s):  
Irma Sánchez-Vargas ◽  
Jaclyn C. Scott ◽  
B. Katherine Poole-Smith ◽  
Alexander W. E. Franz ◽  
Valérie Barbosa-Solomieu ◽  
...  

2006 ◽  
Vol 139 (3) ◽  
pp. 607-614 ◽  
Author(s):  
Chie Aoki ◽  
Kazuya I.P.J. Hidari ◽  
Saki Itonori ◽  
Akihiro Yamada ◽  
Naonori Takahashi ◽  
...  

1990 ◽  
Vol 43 (3) ◽  
pp. 308-313 ◽  
Author(s):  
Isabelle Tardieux ◽  
Laurent Lapchin ◽  
François Rodhain ◽  
Olivier Poupel

2006 ◽  
Vol 87 (8) ◽  
pp. 2263-2268 ◽  
Author(s):  
Dennis J. Pierro ◽  
Ma Isabel Salazar ◽  
Barry J. Beaty ◽  
Ken E. Olson

A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in Aedes aegypti mosquitoes. During construction, an isoleucine to methionine (Ile→Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. In vitro-transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.


2018 ◽  
Vol 12 (8) ◽  
pp. e0006754 ◽  
Author(s):  
Irma Sánchez-Vargas ◽  
Laura C. Harrington ◽  
Jeffrey B. Doty ◽  
William C. Black ◽  
Ken E. Olson

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