Faculty Opinions recommendation of Pias3-dependent SUMOylation directs rod photoreceptor development.

2009 ◽  
Author(s):  
Carole LaBonne
Keyword(s):  
Rod Photoreceptor ◽  
Photoreceptor Development

scholarly journals Retinoid-related orphan nuclear receptor ROR  is an early-acting factor in rod photoreceptor development

2009 ◽  
Vol 106 (41) ◽  
pp. 17534-17539 ◽  
Author(s):  
L. Jia ◽  
E. C. T. Oh ◽  
L. Ng ◽  
M. Srinivas ◽  
M. Brooks ◽  
...  
Keyword(s):  
Nuclear Receptor ◽  
Rod Photoreceptor ◽  
Orphan Nuclear Receptor ◽  
Photoreceptor Development

scholarly journals Her9/Hes4 is required for retinal photoreceptor development, maintenance, and survival

2019 ◽  
Author(s):  
Cagney E. Coomer ◽  
Stephen G. Wilson ◽  
Kayla F. Titialii-Torres ◽  
Jessica D. Bills ◽  
Laura A. Krueger ◽  
...  
Keyword(s):  
Marginal Zone ◽  
Rod Photoreceptor ◽  
Lineage Specification ◽  
Extrinsic Factors ◽  
Helix Loop Helix ◽  
Retinal Photoreceptor ◽  
Vertebrate Photoreceptor ◽  
Photoreceptor Development ◽  
Ciliary Marginal Zone

AbstractThe intrinsic and extrinsic factors that regulate vertebrate photoreceptor specification and differentiation are complex, and our understanding of all the players is far from complete. Her9, the zebrafish ortholog of human HES4, is a basic helix-loop-helix-orange (bHLH-O) transcriptional repressor that regulates neurogenesis in several developmental contexts. We have previously shown that her9 is upregulated during chronic rod photoreceptor degeneration and regeneration in adult zebrafish, but little is known about the role of her9 during retinal development. To better understand the function of Her9 in the retina, we generated zebrafish her9 CRISPR mutants. Her9 homozygous mutants displayed striking retinal phenotypes, including decreased numbers of rods and red/green cones, whereas blue and UV cones were relatively unaffected. The reduction in rods and red/green cones correlated with defects in photoreceptor subtype lineage specification. The remaining rods and double cones displayed abnormally truncated outer segments, and elevated levels of apoptosis. In addition to the photoreceptor defects, her9 mutants also possessed a reduced proliferative ciliary marginal zone, and decreased and disorganized Müller glia. Mutation of her9 was larval lethal, with no mutants surviving past 13 days post fertilization. Our results reveal a previously undescribed role for Her9/Hes4 in photoreceptor differentiation, maintenance, and survival.

A temporally regulated, diffusible activity is required for rod photoreceptor development in vitro

Development ◽  
1992 ◽  
Vol 114 (4) ◽  
pp. 947-957 ◽  
Author(s):  
D. Altshuler ◽  
C. Cepko
Keyword(s):  
Cell Fate ◽  
Rod Photoreceptor ◽  
Cell Type ◽  
Retinal Neuron ◽  
Development In Vitro ◽  
Temporally Correlated ◽  
Photoreceptor Development

The retina is a relatively simple and well-characterized CNS structure in which cell-cell interactions have been hypothesized to influence cell type determination. By manipulating cell density in serum-free cultures we show that rat rod photoreceptor development requires a diffusible activity produced by neonatal retinal cells. This effect is not mediated by changes in cell survival or mitosis. Production of the rod promoting activity varies with developmental stage and is temporally correlated with the timing of rod generation in vivo. In low density cultures, which do not support rod development, an increased fraction of cells stain with an antibody specific for another retinal neuron, the bipolar cell. Thus, the diffusible rod promoting activity may influence cell fate determination, and not only terminal differentiation. These results provide an approach for the molecular characterization of developmentally important signals in the vertebrate retina.

scholarly journals Rb regulates proliferation and rod photoreceptor development in the mouse retina

2004 ◽  
Vol 36 (4) ◽  
pp. 351-360 ◽  
Author(s):  
Jiakun Zhang ◽  
Jonathan Gray ◽  
Lizhao Wu ◽  
Gustavo Leone ◽  
Sheldon Rowan ◽  
...  
Keyword(s):  
Mouse Retina ◽  
Rod Photoreceptor ◽  
Photoreceptor Development

Ciliary neurotrophic factor promotes chick photoreceptor development in vitro

Development ◽  
1995 ◽  
Vol 121 (8) ◽  
pp. 2695-2706 ◽  
Author(s):  
S. Fuhrmann ◽  
M. Kirsch ◽  
H.D. Hofmann
Keyword(s):  
Growth Factor ◽  
Neurotrophic Factor ◽  
Ciliary Neurotrophic Factor ◽  
Rod Photoreceptor ◽  
Opsin Expression ◽  
Chick Retina ◽  
Continuous Presence ◽  
Development In Vitro ◽  
Photoreceptor Development

Previous in vitro studies have convincingly demonstrated the involvement of diffusible factors in the regulation of photoreceptor development. We now provide evidence that ciliary neurotrophic factor (CNTF) represents one of these regulatory molecules. In low density monolayer cultures prepared from embryonic day 8 chick retina, photoreceptor development was studied using the monoclonal antiopsin antibody rho-4D2 as a differentiation marker. The number of cells acquiring opsin immunoreactivity, determined after 3 days in vitro, was increased up to 4-fold in the presence of CNTF to maximally 10.5% of all cells. Basic fibroblast growth factor or taurine both of which have been reported to stimulate opsin expression in rat retinal cultures and other neurotrophic factors tested (nerve growth factor, brain derived neurotrophic factor) had no effect. The EC50 of the CNTF effect (2.6 pM) was virtually identical to that measured for other CNTF receptor mediated cellular responses. Conditioned medium produced by cultured retinal cells (most likely glial cells) exhibited opsin stimulating activity identical to that of CNTF. Stimulation of opsin expression was specific for morphologically less mature photoreceptors and obviously restricted to rods, since changes in the number of identifiable cone photoreceptors expressing opsin immunoreactivity (10% of all cones) were not detectable. Measurement of the kinetics of the CNTF response revealed that the factor acted on immature opsin-negative progenitors and that CNTF effects were unlikely to reflect enhanced cell survival. Proliferation of photoreceptors was also unaffected, as demonstrated by [3H]thymidine autoradiography. With prolonged culture periods a gradual decrease in the number of opsin-positive cells was observed both in controls and in the continuous presence of CNTF. This decrease could be partly prevented by the addition of 1 mM taurine. Our results suggest that CNTF acted as an inductive signal for uncommitted progenitor cells or during early stages of rod photoreceptor differentiation, whereas other extrinsic stimulatory activities seemed to be required for further maturation.

scholarly journals Pias3-Dependent SUMOylation Directs Rod Photoreceptor Development

Neuron ◽  
2009 ◽  
Vol 61 (2) ◽  
pp. 234-246 ◽  
Author(s):  
Akishi Onishi ◽  
Guang-Hua Peng ◽  
Chengda Hsu ◽  
Uel Alexis ◽  
Shiming Chen ◽  
...  
Keyword(s):  
Rod Photoreceptor ◽  
Photoreceptor Development
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