Faculty Opinions recommendation of EB virus-induced B lymphocyte cell lines producing specific antibody.

Recent studies suggest that some T and B lymphocyte cell lines bind to the integrin lymphocyte function-associated molecule 1 (LFA-1) chiefly through a pathway independent of its two known counter-receptors, intercellular adhesion molecules (ICAMs)-1 and -2. A monoclonal antibody (mAb) was raised that, in combination with blocking mAb to ICAM-1 and ICAM-2, can completely inhibit binding of these cell lines to purified LFA-1. This third ligand, designated ICAM-3 based on its functional relatedness to ICAM-1 and -2, is a highly glycosylated protein of 124,000 Mr. It is well expressed on all leukocytes and absent from endothelial cells. In assays of adhesion of resting lymphocytes to purified LFA-1, ICAM-3 is by far the most functionally important ICAM, implying an important role for ICAM-3 in the generation of immune responses.

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Tumor promoter phorbol myristic acetate stimulates immunoglobulin secretion correlated with growth cessation in human B lymphocyte cell lines.

Journal of Clinical Investigation ◽

10.1172/jci110332 ◽

1981 ◽

Vol 68 (4) ◽

pp. 1093-1096 ◽

Cited By ~ 23

Author(s):

P Ralph ◽

T Kishimoto

Keyword(s):

Cell Lines ◽

B Lymphocyte ◽

Tumor Promoter ◽

Growth Cessation ◽

Immunoglobulin Secretion ◽

Lymphocyte Cell

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Discrimination between human populations using a small number of differentially methylated CpG sites: a preliminary study using lymphoblastoid cell lines and peripheral blood samples of European and Chinese origin

BMC Genomics ◽

10.1186/s12864-020-07092-x ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Patrycja Daca-Roszak ◽

Roman Jaksik ◽

Julia Paczkowska ◽

Michał Witt ◽

Ewa Ziętkiewicz

Keyword(s):

Cell Lines ◽

Peripheral Blood ◽

B Lymphocyte ◽

Methylation Status ◽

Predictive Ability ◽

Human Populations ◽

Lymphoblastoid Cell Lines ◽

Blood Samples ◽

Cpg Sites ◽

Lymphocyte Cell

Abstract Background Epigenetics is one of the factors shaping natural variability observed among human populations. A small proportion of heritable inter-population differences are observed in the context of both the genome-wide methylation level and the methylation status of individual CpG sites. It has been demonstrated that a limited number of carefully selected differentially methylated sites may allow discrimination between main human populations. However, most of the few published results have been performed exclusively on B-lymphocyte cell lines. Results The goal of our study was to identify a set of CpG sites sufficient to discriminate between populations of European and Chinese ancestry based on the difference in the DNA methylation profile not only in cell lines but also in primary cell samples. The preliminary selection of CpG sites differentially methylated in these two populations (pop-CpGs) was based on the analysis of two groups of commercially available ethnically-specific B-lymphocyte cell lines, performed using Illumina Infinium Human Methylation 450 BeadChip Array. A subset of 10 pop-CpGs characterized by the best differentiating criteria (|Mdiff| > 1, q < 0.05; lack of the confounding genomic features), and 10 additional CpGs in their immediate vicinity, were further tested using pyrosequencing technology in both B-lymphocyte cell lines and in the primary samples of the peripheral blood representing two analyzed populations. To assess the population-discriminating potential of the selected set of CpGs (further referred to as “composite pop (CEU-CHB)-CpG marker”), three classification methods were applied. The predictive ability of the composite 8-site pop (CEU-CHB)-CpG marker was assessed using 10-fold cross-validation method on two independent sets of samples. Conclusions Our results showed that less than 10 pop-CpG sites may distinguish populations of European and Chinese ancestry; importantly, this small composite pop-CpG marker performs well in both lymphoblastoid cell lines and in non-homogenous blood samples regardless of a gender.

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Abnormal surface expression of sialoglycans on B lymphocyte cell lines from patients with carbohydrate deficient glycoprotein syndrome I A (CDGS I A)

Glycobiology ◽

10.1093/glycob/8.10.963 ◽

1998 ◽

Vol 8 (10) ◽

pp. 963-972 ◽

Cited By ~ 11

Author(s):

M. Bergmann ◽

H.-J. Gross ◽

F. Abdelatty ◽

P. Moller ◽

J. Jaeken ◽

...

Keyword(s):

Cell Lines ◽

B Lymphocyte ◽

Surface Expression ◽

Carbohydrate Deficient Glycoprotein Syndrome ◽

Lymphocyte Cell

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Discrimination between human populations using a small number of differentially methylated CpG sites: A preliminary study using lymphoblastoid cell lines and peripheral blood samples of European and Chinese origin.

10.21203/rs.3.rs-20643/v2 ◽

2020 ◽

Author(s):

Patrycja Daca-Roszak ◽

Roman Jaksik ◽

Julia Paczkowska ◽

Michal Witt ◽

Ewa Zietkiewicz

Keyword(s):

Cell Lines ◽

Peripheral Blood ◽

B Lymphocyte ◽

Methylation Status ◽

Human Populations ◽

Lymphoblastoid Cell Lines ◽

Blood Samples ◽

Chinese Populations ◽

Cpg Sites ◽

Lymphocyte Cell

Abstract Background Epigenetics is one of the factors shaping natural variability observed among human populations. A small proportion of heritable inter-population differences are observed in the context of both the genome-wide methylation level and the methylation status of individual CpG sites. It has been demonstrated that a limited number of carefully selected differentially methylated sites may allow discrimination between main human populations. However, most of the few published results have been performed exclusively on B-lymphocyte cell lines. Results The goal of our study was to identify a set of CpG sites sufficient to discriminate between European and Chinese populations based on the difference in the DNA methylation profile not only in cell lines but also in primary cell samples. The preliminary selection of CpG sites differentially methylated in these two populations (pop-CpGs) was based on the analysis of two groups of commercially available ethnically-specific B-lymphocyte cell lines, performed using Illumina Infinium Human Methylation 450 BeadChip Array. A subset of ten pop-CpGs characterized by the best differentiating criteria (|Mdiff|>1, q<0.05; lack of the confounding genomic features), and ten additional CpGs in their immediate vicinity, were further tested using pyrosequencing technology in both B-lymphocyte cell lines and in the primary samples of the peripheral blood representing two analyzed populations. To assess the population-discriminating potential of the selected set of CpGs (further referred to as “ composite pop(CEU-CHB)-CpG marker ”) , three classification methods were applied. The predictive ability of the composite 8-site pop(CEU-CHB)-CpG marker was assessed using 10-fold cross-validation method on two independent sets of samples. Conclusions Our results showed that less than 10 pop-CpG sites may distinguish European and Chinese populations; importantly, this small composite pop-CpG marker performs well in both lymphoblastoid cell lines and in non-homogenous blood samples regardless of a gender .

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