Faculty Opinions recommendation of Anti-inflammatory effect of an Escherichia coli extract in a mouse model of lipopolysaccharide-induced cystitis.

Barringtonia augusta methanol extract (Ba-ME) is a folk medicine found in the wetlands of Thailand that acts through an anti-inflammatory mechanism that is not understood fully. Here, we examine how the methanol extract of Barringtonia augusta (B. augusta) can suppress the activator protein 1 (AP-1) signaling pathway and study the activities of Ba-ME in the lipopolysaccharide (LPS)-treated RAW264.7 macrophage cell line and an LPS-induced peritonitis mouse model. Non-toxic concentrations of Ba-ME downregulated the mRNA expression of cytokines, such as cyclooxygenase and chemokine ligand 12, in LPS-stimulated RAW264.7 cells. Transfection experiments with the AP-1-Luc construct, HEK293T cells, and luciferase assays were used to assess whether Ba-ME suppressed the AP-1 functional activation. A Western blot assay confirmed that C-Jun N-terminal kinase is a direct pharmacological target of Ba-ME action. The anti-inflammatory effect of Ba-ME, which functions by β-activated kinase 1 (TAK1) inhibition, was confirmed by using an overexpression strategy and a cellular thermal shift assay. In vivo experiments in a mouse model of LPS-induced peritonitis showed the anti-inflammatory effect of Ba-ME on LPS-stimulated macrophages and acute inflammatory mouse models. We conclude that Ba-ME is a promising anti-inflammatory drug targeting TAK1 in the AP-1 pathway.

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Anti-inflammatory Effect of Arginase Inhibitor and Corticosteroid on Airway Allergic Reactions in a Dermatophogoides farinae-induced NC/Nga Mouse Model

Inflammation ◽

10.1007/s10753-012-9529-3 ◽

2012 ◽

Vol 36 (1) ◽

pp. 141-151 ◽

Cited By ~ 5

Author(s):

Keiki Ogino ◽

Masayuki Kubo ◽

Hidekazu Takahashi ◽

Ran Zhang ◽

Yu Zou ◽

...

Keyword(s):

Mouse Model ◽

Allergic Reactions ◽

Anti Inflammatory ◽

Inflammatory Effect

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Anti-inflammatory Effect of Astragalin and Chlorogenic Acid on Escherichia coli-Induced Inflammation of Sheep Endometrial Epithelium Cells

Frontiers in Veterinary Science ◽

10.3389/fvets.2020.00201 ◽

2020 ◽

Vol 7 ◽

Cited By ~ 1

Author(s):

Xuequan Hu ◽

Meng Wang ◽

Yangyang Pan ◽

Yingying Xie ◽

Jinhui Han ◽

...

Keyword(s):

Escherichia Coli ◽

Chlorogenic Acid ◽

Anti Inflammatory ◽

Endometrial Epithelium ◽

Epithelium Cells ◽

Inflammatory Effect

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The Proteolytic Fraction From Vasconcellea cundinamarcensis Latex Displays Anti-Inflammatory Effect in A Mouse Model of Acute TNBS-Induced Colitis

Scientific Reports ◽

10.1038/s41598-020-59895-3 ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

Ronniel Morais Albuquerque ◽

Marina Passos Pizzitola ◽

Ana Cândida Araújo e Silva ◽

Dalton Dittz ◽

Kátia Michelle Freitas ◽

...

Keyword(s):

Mouse Model ◽

Anti Inflammatory ◽

Inflammatory Effect

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The aerial part of Taraxacum coreanum extract has an anti-inflammatory effect on peritoneal macrophages in vitro and increases survival in a mouse model of septic shock

Journal of Ethnopharmacology ◽

10.1016/j.jep.2012.12.009 ◽

2013 ◽

Vol 146 (1) ◽

pp. 1-8 ◽

Cited By ~ 13

Author(s):

Mi-Hwa Lee ◽

Hee Kang ◽

Kyungjin Lee ◽

Gabsik Yang ◽

Inhye Ham ◽

...

Keyword(s):

Septic Shock ◽

Mouse Model ◽

Aerial Part ◽

Peritoneal Macrophages ◽

Anti Inflammatory ◽

Taraxacum Coreanum ◽

Inflammatory Effect

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Action of anti-inflammatory agents on endotoxin-induced lung inflammation in mice

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1959.197.4.803 ◽

1959 ◽

Vol 197 (4) ◽

pp. 803-807 ◽

Cited By ~ 2

Author(s):

Ernest C. Herrmann ◽

Claire Engle ◽

Preston L. Perlman

Keyword(s):

Escherichia Coli ◽

Blood Vessel ◽

Lung Inflammation ◽

Lung Weight ◽

Vessel Permeability ◽

Anti Inflammatory ◽

Inflammatory System ◽

Modest Effect ◽

Inflammatory Effect ◽

Severe Lung

The instillation of Escherichia coli endotoxin (lipopolysaccharide) intranasally into mice produced severe lung lesions and a substantial increase in lung weight. Lung inflammation was maximal about 3 days after endotoxin instillation. Intraperitoneal injection of prednisone, cortisone and hydrocortisone sharply suppressed the lung inflammation. Five milligrams of prednisone produced a maximal anti-inflammatory effect when injected 24 and 48 hours after the endotoxin. Administration of large doses of Butazolidin also produced a modest effect on the lung inflammation. It is suggested that locally applied endotoxin alters blood vessel permeability of the lungs, resulting in hemorrhage, edema and exudation. The anti-inflammatory steroids reverse or inhibit this effect, perhaps by maintaining the integrity of the capillaries. Possible use and significance of this experimental anti-inflammatory system is discussed.

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IFN-τDisplays Anti-Inflammatory Effects onStaphylococcus aureusEndometritis via Inhibiting the Activation of the NF-κB and MAPK Pathways in Mice

BioMed Research International ◽

10.1155/2017/2350482 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12

Author(s):

Zhenbiao Zhang ◽

Yingfang Guo ◽

Yuzhu Liu ◽

Chengye Li ◽

Mengyao Guo ◽

...

Keyword(s):

Mouse Model ◽

Protective Effect ◽

Inflammatory Cytokine ◽

Inflammatory Diseases ◽

Potential Drug ◽

Histopathological Changes ◽

Uterine Infection ◽

Anti Inflammatory ◽

Anti Inflammatory Cytokine ◽

Inflammatory Effect

The aim of the present study was to determine the anti-inflammatory effect of IFN-τon endometritis using a mouse model ofS. aureus-induced endometritis and to elucidate the mechanism of action underlying these effects. In the present study, the effect of IFN-τonS. aureusgrowth was monitored by turbidimeter at 600 nm. IFN-τdid not affectS. aureusgrowth. The histopathological changes indicated that IFN-τhad a protective effect on uterus tissues withS. aureusinfection. The ELISA and qPCR results showed the production of the proinflammatory cytokines TNF-α, IL-1β, and IL-6 was decreased with IFN-τtreatment. In contrast, the level of the anti-inflammatory cytokine IL-10 was increased. We further studied the signaling pathway associated with these observations, and the qPCR results showed that the expression of TLR2 was repressed by IFN-τ. Furthermore, the western blotting results showed the phosphorylation of IκB, NF-κB p65, and MAPKs (p38, JNK, and ERK) was inhibited by IFN-τtreatment. The results suggested that IFN-τmay be a potential drug for the treatment of uterine infection due toS. aureusor other infectious inflammatory diseases.

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