Faculty Opinions recommendation of Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.

The possible protection of cultured human cells from acute dioxin injury by antioxidants was investigated. The most potent dioxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), caused vacuolization of the smooth endoplasmic reticulum and Golgi apparatus in cultured human conjunctival epithelial cells and cervical cancer cells. Subsequent nuclear damage included a deep irregular indentation resulting in cell death. A dosage of 30–40 ng/mL TCDD induced maximal intracellular production of H2O2 at 30 minutes and led to severe cell death (0–31% survival) at two hours. A dose of 1.7 mM alpha-tocopherol or 1 mM L-dehydroascorbic acid significantly protected human cells against acute TCDD injuries (78–97% survivals), but vitamin C did not provide this protection. These results indicate that accidental exposure to fatal doses of TCDD causes cytoplasmic free radical production within the smooth endoplasmic reticular systems, resulting in severe cytotoxicity, and that vitamin E and dehydroascorbic acid can protect against TCDD-induced cell damage.

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High Dynamic Imaging of Luminous Massive Stars

EAS Publications Series ◽

10.1051/eas:2003050 ◽

2003 ◽

Vol 8 ◽

pp. 49-54

Author(s):

F. Vakili

Keyword(s):

Massive Stars ◽

Dynamic Imaging ◽

High Dynamic

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Chromium oxidation state mapping in human cells

Journal de Physique IV (Proceedings) ◽

10.1051/jp4:200300082 ◽

2003 ◽

Vol 104 ◽

pp. 289-292 ◽

Cited By ~ 2

Author(s):

R. Ortega ◽

B. Fayard ◽

M. Salomé ◽

G. Devès ◽

J. Susini

Keyword(s):

Oxidation State ◽

Human Cells ◽

Chromium Oxidation

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Transport of clozapine and its major metabolites into human cells of glial and neuronal origin

Pharmacopsychiatry ◽

10.1055/s-2003-825367 ◽

2004 ◽

Vol 36 (05) ◽

Author(s):

U Henning ◽

K Krieger ◽

S Loeffler ◽

A Klimke

Keyword(s):

Human Cells

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Lineage conversion of human cells to an adrenocortical phenotype: a new technology to study the adrenal gland

Endocrine Abstracts ◽

10.1530/endoabs.38.p415 ◽

2015 ◽

Author(s):

Gerard Ruiz Babot ◽

Irene Hadjidemetriou ◽

Sharon Jane Ajodha ◽

David Taylor ◽

Norman Taylor ◽

...

Keyword(s):

Adrenal Gland ◽

New Technology ◽

Human Cells

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Contrast-Enhanced Ultrasound: a Simple and Effective Tool in Defining a Rapid Diagnostic Work-up for Small Nodules Detected in Cirrhotic Patients during Surveillance

Journal of Gastrointestinal and Liver Diseases ◽

10.15403/jgld.2014.1121.252.chu ◽

2016 ◽

Vol 25 (2) ◽

pp. 205-211 ◽

Cited By ~ 13

Author(s):

Antonio Giorgio ◽

Luca Montesarchio ◽

Piero Gatti ◽

Ferdinando Amendola ◽

Paolo Matteucci ◽

...

Keyword(s):

Predictive Value ◽

Imaging Modality ◽

Dynamic Imaging ◽

Contrast Enhanced Ultrasound ◽

Histologic Diagnosis ◽

Therapeutic Work ◽

Contrast Enhanced ◽

Arterial Vascularization ◽

Cirrhotic Patients ◽

Work Up

Background & Aims: Disappearance of portal blood flow and arterial vascularization is the hallmark of hepatocarcinogenesis. The capability of a dynamic imaging modality detecting arterial hypervascularization of small nodules is crucial to promote a rapid diagnostic and therapeutic work-up improving survival. We aimed to evaluate the capability of CEUS to detect arterial vascularization of ≤ 2 cm HCC nodules arising during surveillance so as to shorten the diagnostic and therapeutic work-up. Methods: From October 2009 to September 2014, among 1757 consecutive cirrhotic patients under surveillance with ultrasound (US), 243 patients had new single nodules 7-20 mm; 229/243 had a conclusive histologic diagnosis and comprised the study group. All patients underwent CEUS followed by enhanced MRI and US guided percutaneous 18G needle core biopsy of the nodules. Of the 229 nodules, 27 were hyperechoic, 171 hypoechoic and 31 isoechoic lesions. Results: The histology results revealed that 199/229 nodules were HCC and 30 were benign. Of 199 HCC, CEUS evidenced arterial hypervascularity in 190 nodules (95.5%) (sensitivity 94.48 %, specificity 100%, PPV 100%, NPV 76.92 %). Of the 39 CEUS arterial-unenhanced nodules, 30 were benign and 9 (23%) were well-differentiated HCC. eMRI showed arterial hypervascularity in 199 nodules (86,9%). Of these, only 193 (97%) were histologically HCCs while 6 were benign (sensitivity: 97%, specificity: 80%, PPV: 97%, NPV: 80%). Conclusions: CEUS has a great capability to detect arterial hypervascularity of small HCC. Because only 4.5% of new nodules escape the demonstration of arterial hyervascularity, CEUS must be performed immediately after conventional US to contrast the malignant fate of small lesions arising in a cirrhotic liver.. Abbreviations: CEUS: contrast-enhanced ultrasound; CT: computed tomography; HCC: hepatocellular carcinoma;MRI: magnetic resonance; NPV: negative predictive value; PPV: positive predictive value; US: ultrasonography.

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