Faculty Opinions recommendation of Analysis of skin testing and serum-specific immunoglobulin E to predict airway reactivity to cat allergens.

Background Allergists commonly perform intradermal skin testing (IDST) after negative skin-prick testing (SPT) to comprehensively diagnose environmental allergic sensitization. However, with the availability of modern methods to detect serum-specific immunoglobulin E (ssIgE), it is unclear if ssIgE testing could substitute for IDST. Objective To determine the efficacy of ssIgE testing and IDST when added to SPT in diagnosing environmental allergic sensitizations. Methods SPT, IDST, and ssIgE testing to nine common environmental allergens were analyzed in 75 patients with oculonasal symptoms who presented to our allergy clinics in the Bronx, New York, between January 2014 and May 2015. Results A total of 651 SPT and 499 ssIgE tests were independently performed and revealed 162 (25%) and 127 (25%) sensitizations, respectively. When SPT results were negative, IDST results revealed 108 of 452 additional sensitizations (24%). In contrast, when SPT results were negative, ssIgE test results only revealed 9% additional sensitizations. When both SPT and IDST results were negative, ssIgE testing only detected 3% of additional sensitizations, and ssIgE levels were typically low in these cases (median, 1.25 kU/L; range, 0.357–4.47 kU/L). When both SPT and ssIgE test results were negative, IDST results detected 15% additional sensitizations. Conclusion IDST detected more additional environmental sensitizations compared with ssIgE testing. IDST, therefore, may be useful when the SPT and/or ssIgE testing results were negative, but the exposure history indicated relevant allergic sensitization. Serology added only a little more information if both SPT and IDST results were negative but may be useful in combination with SPT if IDST cannot be performed.

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Comparison of the diagnostic value of two assays for specific immunoglobulin E in suspected beta lactam allergy

Medicinski pregled ◽

10.2298/mpns1708209k ◽

2017 ◽

Vol 70 (7-8) ◽

pp. 209-215 ◽

Cited By ~ 1

Author(s):

Mitja Kosnik ◽

Mira Silar ◽

Mihaela Zidarn ◽

Petar Korosec

Keyword(s):

Immunoglobulin E ◽

Skin Testing ◽

Diagnostic Value ◽

Provocation Tests ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

Penicillin Treatment ◽

Provocation Testing ◽

Drug Provocation Tests ◽

Total Immunoglobulin

Introduction. The aim of this study was to compare the diagnostic value of two imunoglobulin E assays for penicillin in a group of patients with a history compatible with hypersensitivity reaction during penicillin treatment who exhibited positive skin testing and/or a drug provocation testing. Material and Methods. In the first part of the study, penicillin G, V and/or amoxicillin specific immunoglobulin E, positive with ImmunoCAP system (Termo Fisher, Waltham, Massachusetts, USA), were selected from our biobank sera, and measured specific immunoglobulin E with Immulite system (Siemens, Munich, Germany). The second part of the study included skin testing, and if negative, drug provocation testing was done with the culprit penicillin in patients with a history compatible with hyper?sensitivity reaction during penicillin treatment. To check the impact of high values of total immunoglobulin E on performance of specific immunoglobulin E, we examined the specific immunoglobulin E to penicillin using both methods in sera of penicillin tolerant patients with total immunoglobulin E over 700 kIU/L. Results. Out of 2.521 in vitro specific immunoglobulin E penicillin tests, 58 were positive with ImmunoCAP. Of these tests, 52 were tested by the Immulite and they were all negative. Among 114 patients with a history compatible with hypersensitivity reac?tion during penicillin treatment, hypersensitivity was confirmed in 36 (31.6%). In 11 patients with positive immediate skin or drug provocation tests, ImmunoCAP was positive in 3 patients and Immulite in 1 patient. Sensitivity of ImmunoCAP and Immulite were 27.1% and 9.1%, respec?tively. Specificity of ImmunoCAP and Immulite were 96.1% and 100%, respectively. Specific immunoglobulin E to penicillins was detected with ImmunoCAP in 8/22 sera with total immunoglobulin E over 700 kIU/L. All sera were negative by the Immulite. Conclusion. Both the Immuno?CAP and Immulite specific immunoglobulin E assays have low sensitiv?ity when assessing patients with a history of immediate hypersensitivity to penicillin, positive immediate skin, or drug provocation tests.

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Comparison Between Intradermal Skin Testing and Serum Specific Immunoglobulin E in Detecting Allergic Sensitization in Patients with Negative Skin Prick Tests

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2014.12.1118 ◽

2015 ◽

Vol 135 (2) ◽

pp. AB57

Author(s):

Denisa Ferastraoaru ◽

Maria Shtessel ◽

Golda Hudes ◽

Gabriele De Vos

Keyword(s):

Immunoglobulin E ◽

Skin Testing ◽

Allergic Sensitization ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

Skin Prick Tests

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Optimal cutoff values of allergen-specific immunoglobulin E to house dust mites and animal dander based on skin-prick test results: Analysis in 16,209 patients with allergic rhinitis

American Journal of Rhinology and Allergy ◽

10.2500/ajra.2017.31.4483 ◽

2017 ◽

Author(s):

S. D. Hong ◽

G. Ryu ◽

M. Y. Seo ◽

J. I. Jeong ◽

H. Y. Kim ◽

...

Keyword(s):

Allergic Rhinitis ◽

Immunoglobulin E ◽

House Dust Mites ◽

Test Results ◽

Dust Mites ◽

Optimal Cutoff ◽

Prick Test ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

Animal Dander

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Allergic diseases attributable to atopy in a population sample of Asian children

Scientific Reports ◽

10.1038/s41598-021-95579-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Chao-Yi Wu ◽

Hsin-Yi Huang ◽

Wen-Chi Pan ◽

Sui-Ling Liao ◽

Man-Chin Hua ◽

...

Keyword(s):

Immunoglobulin E ◽

Population Attributable Risk ◽

Population Sample ◽

International Study ◽

Allergic Diseases ◽

Population Based ◽

School Age Children ◽

Specific Immunoglobulin E ◽

Asian Children ◽

Specific Immunoglobulin

AbstractThe proportion of allergic diseases attributable to atopy remains a subject of controversy. This study aimed to estimate the population risk of physician-diagnosed asthma, rhinitis and eczema attributed to atopy among a population sample of Asian school-age children. Asian children aged 5–18 years (n = 1321) in the Prediction of Allergies in Taiwanese CHildren (PATCH) study were tested for serum allergen-specific immunoglobulin E. Physician-diagnosed asthma, rhinitis and eczema were assessed by a modified International Study of Asthma and Allergies in Childhood questionnaire. Atopy was defined as the presence of serum allergen-specific immunoglobulin E. In this population-based study, 50.4% of the subjects with asthma, 46.3% with rhinitis, and 46.7% with eczema were attributable to atopy. The population attributable risk (PAR) of atopy for three allergic diseases was higher in adolescents (asthma, 54.4%; rhinitis, 59.6%; eczema, 49.5%) than younger children aged less than 10 years (asthma, 46.9%; rhinitis, 39.5%; eczema, 41.9%). Among the seven allergen categories, sensitization to mites had the highest PARs for all three allergic diseases (51.3 to 64.1%), followed by sensitization to foods (asthma, 7.1%; rhinitis, 10.4%; eczema 27.7%). In conclusion, approximately half (46.3 to 50.4%) of Asian children in Taiwan with allergic diseases are attributable to atopy.

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Effect of Specific Immunoglobulin E Response and Comorbidities on Effectiveness of MP-AzeFlu in a Real-Life Study

International Archives of Allergy and Immunology ◽

10.1159/000508749 ◽

2020 ◽

Vol 181 (10) ◽

pp. 754-764

Author(s):

Ludger Klimek ◽

David Price ◽

Gabriella Gálffy ◽

Melanie Emmeluth ◽

Arkady Koltun ◽

...

Keyword(s):

Immunoglobulin E ◽

Real Life ◽

Life Study ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

Real Life Study

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Total and Specific Immunoglobulin E and Their Relationship to Respiratory Symptoms in Quebec Children and Adolescents

Canadian Respiratory Journal ◽

10.1155/2005/248714 ◽

2005 ◽

Vol 12 (8) ◽

pp. 426-432 ◽

Cited By ~ 6

Author(s):

Benoît Levesque ◽

Jean-François Duchesne ◽

Suzanne Gingras ◽

Pierre Allard ◽

Edgar Delvin ◽

...

Keyword(s):

Children And Adolescents ◽

Respiratory Symptoms ◽

Immunoglobulin E ◽

Geometric Mean ◽

Allergic Sensitization ◽

Population Sample ◽

Ethnic Origin ◽

Total Ige ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

BACKGROUND: Respiratory disease is a major cause of morbidity in young people. It is now recognized that atopy plays an important role in the development of chronic respiratory symptoms in children.OBJECTIVE: To examine the determinants and consequences of serum total and specific immunoglobulin E (IgE) in a general population sample of Québec children and adolescents.METHODS: In 1999, 2349 children and adolescents (nine, 13 or 16 years of age) who had participated in a respiratory symptom and disease questionnaire had their total IgE measured. Of these participants, a subsample of 451 children and adolescents was analyzed to detect antibodies to eight specific allergens (ie, allergens of dust mites [Dermatophagoides farinae and Dermatophagoides pteronyssinus], cat, dog, ragweed, Timothy grass, mould [alternaria] and cockroach).RESULTS: The geometric mean of the total IgE was 44.4 U/mL among all participants. Concentrations were higher in boys and increased with age. More than 41% of the participants were sensitized to at least one specific allergen. Such sensitization was strongly associated with the occurrence of respiratory conditions and symptoms, namely asthma, wheezing and rhinitis. Family history, school location and ethnic origin had an impact on the prevalence of atopy and total IgE levels.CONCLUSIONS: Allergic sensitization is a major determinant in the development of asthma, wheezing and rhinitis in children and adolescents in the province of Québec.

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A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of Artemisia-Specific Immunoglobulin E

International Archives of Allergy and Immunology ◽

10.1159/000520511 ◽

2021 ◽

pp. 1-8

Author(s):

Dandan Liu ◽

Bei Zhang ◽

Lina Zhu ◽

Lisheng Zheng ◽

Shaoshen Li ◽

...

Keyword(s):

Food Allergen ◽

Clinical Guideline ◽

Immunoglobulin E ◽

Limit Of Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Chemiluminescence Assay ◽

Limit Of Quantitation ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

Background: Light-initiated chemiluminescence assay (LICA) is a homogeneous assay that has been successfully used for the quantitation of food allergen-specific immunoglobulin E (sIgE), but not inhaled allergen-sIgE. Simultaneously, current assays used to detect allergen-sIgE are serum consuming and/or time consuming. Hence, we established a method for the quantitation of Artemisia-sIgE based on LICA and verified its performance according to the clinical guideline documents, laying a foundation for the quantitation of inhaled and food allergen-sIgE in parallel on LICA. Methods: The assay was established after optimizing the first incubation time and the dilutions of Artemisia-coated chemibeads, biotinylated goat anti-human IgE, and serum. In order to quantitate Artemisia-sIgE, the calibration curve was established with a high positive serum of known concentration. The assay performance was confirmed per the clinical guideline documents. In addition, the correlation between the results of LICA and capture enzyme-linked immunosorbent assay was evaluated. Results: The developed LICA’s coefficients of variation of repeatability and intermediate precision were 3.20%, 2.14%, and 3.85% and 4.30%, 4.00%, and 4.40%, respectively. The limit of detection was 0.10 kUA/L, and the limit of quantitation was 0.11 kUA/L. The range of linearity was from 0.27 kUA/L to 97.53 kUA/L (r = 0.9968). The correlation coefficient (r) for the correlation analysis between results of LICA and capture ELISA was 0.9087. This assay was successfully applied in 64 human serum samples, showing good sensitivity (82.20%) and specificity (100%). Conclusion: An Artemisia-sIgE quantitation assay based on LICA was successfully established. Its performance satisfied the clinical requirements and could be widely used in clinical laboratories.

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