Faculty Opinions recommendation of Nasal chondrocyte-based engineered autologous cartilage tissue for repair of articular cartilage defects: an observational first-in-human trial.

According to data published by the Centers for Disease Control and Prevention, over 6 million people undergo a variety of medical procedures for the repair of articular cartilage defects in the U.S. each year. Trauma, tumor, and age-related degeneration can cause major defects in articular cartilage, which has a poor intrinsic capacity for healing. Therefore, there is substantial interest in the development of novel cartilage tissue engineering strategies to restore articular cartilage defects to a normal or prediseased state. Special attention has been paid to the expansion of chondrocytes, which produce and maintain the cartilaginous matrix in healthy cartilage. This review summarizes the current efforts to generate chondrocytes from adipose-derived stem cells (ASCs) and provides an outlook on promising future strategies.

Download Full-text

Repair of full-thickness articular cartilage defects using IEIK13 self-assembling peptide hydrogel in a non-human primate model

Scientific Reports ◽

10.1038/s41598-021-83208-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Alexandre Dufour ◽

Jérôme E. Lafont ◽

Marie Buffier ◽

Michaël Verset ◽

Angéline Cohendet ◽

...

Keyword(s):

Articular Cartilage ◽

Articular Chondrocytes ◽

Cartilage Tissue ◽

Cartilage Defects ◽

Full Thickness ◽

Primate Model ◽

Self Assembling ◽

Articular Cartilage Defects ◽

Human Primate

AbstractArticular cartilage is built by chondrocytes which become less active with age. This declining function of the chondrocytes, together with the avascular nature of the cartilage, impedes the spontaneous healing of chondral injuries. These lesions can progress to more serious degenerative articular conditions as in the case of osteoarthritis. As no efficient cure for cartilage lesions exist yet, cartilage tissue engineering has emerged as a promising method aiming at repairing joint defects and restoring articular function. In the present work, we investigated if a new self-assembling peptide (referred as IEIK13), combined with articular chondrocytes treated with a chondrogenic cocktail (BMP-2, insulin and T3, designated BIT) could be efficient to restore full-thickness cartilage defects induced in the femoral condyles of a non-human primate model, the cynomolgus monkey. First, in vitro molecular studies indicated that IEIK13 was efficient to support production of cartilage by monkey articular chondrocytes treated with BIT. In vivo, cartilage implant integration was monitored non-invasively by contrast-enhanced micro-computed tomography, and then by post-mortem histological analysis and immunohistochemical staining of the condyles collected 3 months post-implantation. Our results revealed that the full-thickness cartilage injuries treated with either IEIK13 implants loaded with or devoid of chondrocytes showed similar cartilage-characteristic regeneration. This pilot study demonstrates that IEIK13 can be used as a valuable scaffold to support the in vitro activity of articular chondrocytes and the repair of articular cartilage defects, when implanted alone or with chondrocytes.

Download Full-text

TISSUE ENGINEERING AND REGENERATIVE MEDICINE TECHNOLOGIES IN THE TREATMENT OF ARTICULAR CARTILAGE DEFECTS

Russian Journal of Transplantology and Artificial Organs ◽

10.15825/1995-1191-2016-4-102-122 ◽

2017 ◽

Vol 18 (4) ◽

pp. 102-122

Author(s):

Yu. B. Basok ◽

V. I. Sevastianov

Keyword(s):

Tissue Engineering ◽

Articular Cartilage ◽

Regenerative Medicine ◽

Industrial Society ◽

Cartilage Tissue ◽

Cartilage Defects ◽

Limited Capacity ◽

Joint Cartilage ◽

Articular Cartilage Defects ◽

Stimulation Of

Some of the most pressing health problems of the industrial society are the damage and degeneration of articular cartilage associated with the limited capacity of tissues to regenerate. The review describes the existing and developing technologies for the recovery and replacement of damaged joint cartilage tissue. The results obtained are analyzed covering two major areas: the stimulation of regeneration of damaged cartilage tissue and the growing of cartilage tissue elements in bioreactors.

Download Full-text

The Application of Polysaccharide Biocomposites to Repair Cartilage Defects

International Journal of Polymer Science ◽

10.1155/2014/654597 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Feng Zhao ◽

Wei He ◽

Yueling Yan ◽

Hongjuan Zhang ◽

Guoping Zhang ◽

...

Keyword(s):

Hyaluronic Acid ◽

Articular Cartilage ◽

Cartilage Tissue ◽

Cartilage Defects ◽

Self Healing ◽

Porous Network ◽

Group A ◽

Group B ◽

Articular Cartilage Defects ◽

Repair Group

Owing to own nature of articular cartilage, it almost has no self-healing ability once damaged. Despite lots of restore technologies having been raised in the past decades, no repair technology has smoothly substituted for damaged cartilage using regenerated cartilage tissue. The approach of tissue engineering opens a door to successfully repairing articular cartilage defects. For instance, grafting of isolated chondrocytes has huge clinical potential for restoration of cartilage tissue and cure of chondral injury. In this paper, SD rats are used as subjects in the experiments, and they are classified into three groups: natural repair (group A), hyaluronic acid repair (group B), and polysaccharide biocomposites repair (hyaluronic acid hydrogel containing chondrocytes, group C). Through the observation of effects of repairing articular cartilage defects, we concluded that cartilage repair effect of polysaccharide biocomposites was the best at every time point, and then the second best was hyaluronic acid repair; both of them were better than natural repair. Polysaccharide biocomposites have good biodegradability and high histocompatibility and promote chondrocytes survival, reproduction, and spliting. Moreover, polysaccharide biocomposites could not only provide the porous network structure but also carry chondrocytes. Consequently hyaluronic acid-based polysaccharide biocomposites are considered to be an ideal biological material for repairing articular cartilage.

Download Full-text

Costal Chondrocyte–Derived Pellet-Type Autologous Chondrocyte Implantation versus Microfracture for Repair of Articular Cartilage Defects: A Prospective Randomized Trial

Cartilage ◽

10.1177/1947603520921448 ◽

2020 ◽

pp. 194760352092144

Author(s):

Kyoung-Ho Yoon ◽

Jae Doo Yoo ◽

Chong-Hyuk Choi ◽

Jungsun Lee ◽

Jin-Yeon Lee ◽

...

Keyword(s):

Articular Cartilage ◽

Magnetic Resonance ◽

Autologous Chondrocyte Implantation ◽

Cartilage Tissue ◽

Cartilage Defects ◽

Efficacy And Safety ◽

Chondrocyte Implantation ◽

Articular Cartilage Defects ◽

Costal Chondrocyte ◽

Autologous Chondrocyte

Objective To compare the efficacy and safety of costal chondrocyte–derived pellet-type autologous chondrocyte implantation (CCP-ACI) with microfracture (MFx) for repair of articular cartilage defects of the knee. Design Thirty subjects with an International Cartilage Repair Society (ICRS) grade 3 to 4 chondral defect (2-10 cm2 in area; ≤4 cm3 in volume) were randomized at a ratio of 2:1 (CCP-ACI:MFx). Twenty patients were allocated in the CCP-ACI group and 10 patients in the MFx group. CCP-ACI was performed by harvesting costal cartilage at least 4 weeks before surgery. Implantation was performed without any marrow stimulation. Efficacy and safety were assessed at weeks 8, 24, and 48 after surgery according to the magnetic resonance observation of cartilage repair tissue (MOCART) score and clinical outcomes. Results MOCART scores improved from baseline to 24 and 48 weeks postoperatively in both treatment groups. The improvement in MOCART scores in the CCP-ACI group was significantly greater than that in the MFx group at 24 and 48 weeks (39.1 vs 21.8 and 43.0 vs 24.8, respectively). The proportions of complete defect repair and complete integration were significantly higher in the CCP-ACI group than the MFx group at 48 weeks. Improvement in Lysholm score and KOOS subscores, including Function (Sports and Recreational Activity) and knee-related quality of life was significantly greater in the CCP-ACI group than the MFx group at 48 weeks (35.4 vs 31.5, 35.7 vs 28.5, and 27.9 vs 11.6, respectively). Conclusion Treatment of cartilage defects with CCP-ACI yielded satisfactory cartilage tissue repair outcomes, with good structural integration with native cartilage tissue shown by magnetic resonance imaging at 24 and 48 weeks after surgery. Level of Evidence Level 1: Randomized controlled study.

Download Full-text

Functional hybrid organo-inorganic composite materials of the incorporative type for the recovery of articular cartilage defects

Functional materials ◽

10.15407/fm26.03.609 ◽

2019 ◽

Vol 26 (3) ◽

Keyword(s):

Articular Cartilage ◽

Composite Materials ◽

Cartilage Defects ◽

Articular Cartilage Defects ◽

Inorganic Composite

Download Full-text

Faculty Opinions recommendation of Activity levels are higher after osteochondral autograft transfer mosaicplasty than after microfracture for articular cartilage defects of the knee: a retrospective comparative study.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717447889.792802948 ◽

2012 ◽

Author(s):

Edilson Forlin

Keyword(s):

Articular Cartilage ◽

Comparative Study ◽

Cartilage Defects ◽

Activity Levels ◽

Osteochondral Autograft ◽

Osteochondral Autograft Transfer ◽

Retrospective Comparative Study ◽

Articular Cartilage Defects

Download Full-text

A composite scaffold of Wharton’s jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-021-06506-w ◽

2021 ◽

Vol 32 (4) ◽

Author(s):

Zhong Li ◽

Yikang Bi ◽

Qi Wu ◽

Chao Chen ◽

Lu Zhou ◽

...

Keyword(s):

Stem Cells ◽

Articular Cartilage ◽

Composite Scaffold ◽

Biomechanical Properties ◽

Cartilage Defects ◽

Human Umbilical Cord ◽

Composite Scaffolds ◽

Articular Cartilage Defects

AbstractTo evaluate the performance of a composite scaffold of Wharton’s jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out. The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold. In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee. Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs. We found that in vitro, the Young’s modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold. In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs. Both the scaffold and hUCMSCs showed low immunogenicity. These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.

Download Full-text