Faculty Opinions recommendation of Enterococcus faecalis bacteriocin EntV inhibits hyphal morphogenesis, biofilm formation, and virulence of Candida albicans.

2017 ◽  
Author(s):  
Bernhard Hube ◽  
Ilse Jacobsen
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Hyphal Morphogenesis

Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans

2018 ◽  
Vol 46 (2) ◽  
pp. 128-135
Author(s):  
Mohamed Hassan ◽  
Yasmine Tartor ◽  
Ashour Ashour ◽  
Elmowalid Elmowalid
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Cell Free Supernatant ◽  
Hyphal Morphogenesis

scholarly journals Characterization of the mechanism of action of the Enterococcus faecalis bacteriocin EntV on Candida albicans

2021 ◽  
Vol 3 (12) ◽  
Author(s):  
Giuseppe Buda De Cesare ◽  
Yasmin Chebaro ◽  
Shantanu Guha ◽  
Melissa Cruz ◽  
Danielle Garsin ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Bacterial Species ◽  
Positive Bacterium ◽  
Hyphal Morphogenesis ◽  
Target Layer ◽  
Further Development

Candida albicans shares communal niches with multiple bacterial species. Previous work from our group demonstrated that the Gram-positive bacterium Enterococcus faecalis, a normal constituent of the oral and gut microbiome that is often co-isolated with C. albicans, antagonizes hyphal morphogenesis, biofilm formation, and virulence in C. albicans. These effects are mediated by EntV, a bacteriocin and antimicrobial peptide produced by E. faecalis. The main aim of this work is to unveil the molecular mechanism behind the activity of EntV on C. albicans. Using fluorescence microscopy, we determined that EntV binds to the cell walls of several Candida species, including both yeast and hyphae of C. albicans. Contrary to other antimicrobial peptides, it does not cause cell lysis and does not synergize with cell wall damaging agents. Moreover, we screened a library of C. albicans mutants for strains with altered susceptibility to the peptide; most of the positive hits had functions related to cell wall maintenance and were further screened to ascertain changes in the staining patterns. Furthermore, to identify the target layer on the cell wall, pull-down assays were performed. Mannan was identified as the major wall component able to bind the peptide. Finally, live imaging of macrophages incubated with Candida was carried out in order to assess any change in the phagocytic behaviour in presence of the peptide. Identifying the molecular target of EntV in regard to the anti-virulence mechanisms of C. albicans is an important step in its further development as a therapeutic addition to the classical antifungal agents.

scholarly journals Interactions between Candida albicans and Enterococcus faecalis in an Organotypic Oral Epithelial Model

2020 ◽  
Vol 8 (11) ◽  
pp. 1771
Author(s):  
Akshaya Lakshmi Krishnamoorthy ◽  
Alex A. Lemus ◽  
Adline Princy Solomon ◽  
Alex M. Valm ◽  
Prasanna Neelakantan
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Opportunistic Pathogen ◽  
Surface Erosion ◽  
Host Immune System ◽  
Microbial Invasion ◽  
Mucosal Surfaces ◽  
Life Threatening ◽  
Hyphal Formation

Candida albicans as an opportunistic pathogen exploits the host immune system and causes a variety of life-threatening infections. The polymorphic nature of this fungus gives it tremendous advantage to breach mucosal barriers and cause oral and disseminated infections. Similar to C. albicans, Enterococcus faecalis is a major opportunistic pathogen, which is of critical concern in immunocompromised patients. There is increasing evidence that E. faecalis co-exists with C. albicans in the human body in disease samples. While the interactive profiles between these two organisms have been studied on abiotic substrates and mouse models, studies on their interactions on human oral mucosal surfaces are non-existent. Here, for the first time, we comprehensively characterized the interactive profiles between laboratory and clinical isolates of C. albicans (SC5314 and BF1) and E. faecalis (OG1RF and P52S) on an organotypic oral mucosal model. Our results demonstrated that the dual species biofilms resulted in profound surface erosion and significantly increased microbial invasion into mucosal compartments, compared to either species alone. Notably, several genes of C. albicans involved in tissue adhesion, hyphal formation, fungal invasion, and biofilm formation were significantly upregulated in the presence of E. faecalis. By contrast, E. faecalis genes involved in quorum sensing, biofilm formation, virulence, and mammalian cell invasion were downregulated. This study highlights the synergistic cross-kingdom interactions between E. faecalis and C. albicans in mucosal tissue invasion.

scholarly journals The Mycotoxin Zearalenone Hinders Candida albicans Biofilm Formation and Hyphal Morphogenesis

2017 ◽  
Vol 58 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Satish Kumar Rajasekharan ◽  
Jin-Hyung Lee ◽  
Yueju Zhao ◽  
Jintae Lee
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Hyphal Morphogenesis

scholarly journals Piperine Impedes Biofilm Formation and Hyphal Morphogenesis of Candida albicans

2020 ◽  
Vol 11 ◽  
Author(s):  
Arumugam Priya ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Hyphal Morphogenesis
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