Faculty Opinions recommendation of Single-molecule imaging of the transcription factor SRF reveals prolonged chromatin-binding kinetics upon cell stimulation.

2019 ◽  
Author(s):  
Susanne Muehlich ◽  
Melanie Meier
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Binding Kinetics ◽  
Single Molecule Imaging ◽  
Chromatin Binding ◽  
Cell Stimulation

scholarly journals Single-molecule imaging reveals the interplay between transcription factors, nucleosomes, and transcriptional bursting

2018 ◽  
Author(s):  
Benjamin T. Donovan ◽  
Anh Huynh ◽  
David A. Ball ◽  
Michael G. Poirier ◽  
Daniel R. Larson ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Single Molecule ◽  
Target Genes ◽  
Burst Size ◽  
Yeast Cells ◽  
Single Molecule Imaging ◽  
Transcriptional Bursting

SummaryTranscription factors show rapid and reversible binding to chromatin in living cells, and transcription occurs in sporadic bursts, but how these phenomena are related is unknown. Using a combination of in vitro and in vivo single-molecule imaging approaches, we directly correlated binding of the transcription factor Gal4 with the transcriptional bursting kinetics of the Gal4 target genes GAL3 and GAL10 in living yeast cells. We find that Gal4 dwell times sets the transcriptional burst size. Gal4 dwell time depends on the affinity of the binding site and is reduced by orders of magnitude by nucleosomes. Using a novel imaging platform, we simultaneously tracked transcription factor binding and transcription at one locus, revealing the timing and correlation between Gal4 binding and transcription. Collectively, our data support a model where multiple polymerases initiate during a burst as long as the transcription factor is bound to DNA, and a burst terminates upon transcription factor dissociation.

scholarly journals Single-molecule imaging of CREB transcription factor in human cortical-like neurons

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S216-S217
Author(s):  
Yuri Atsumi ◽  
Noriyuki Sugo ◽  
Ryohei Iwata ◽  
Pierre Vanderhaeghen ◽  
Nobuhiko Yamamoto
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Creb Transcription Factor

scholarly journals Single-molecule interaction microscopy reveals antibody binding kinetics

2020 ◽  
Author(s):  
Thilini Perera ◽  
Hirushi Gunasekara ◽  
Ying S. Hu
Keyword(s):  
Single Molecule ◽  
Antibody Binding ◽  
Binding Kinetics ◽  
Biomolecular Interactions ◽  
Single Molecule Imaging ◽  
Cellular Environment ◽  
Nanoscale Topography ◽  
Molecule Interaction ◽  
Limited Duration

Single-molecule imaging has provided new insights on weak transient biomolecular interactions with micromolar to millimolar affinity. However, the limited duration of observation has hindered the study of strong and reversible interactions with sub-nanomolar affinity. We report single-molecule interaction microscopy (SMIM), which combines point accumulation for imaging in nanoscale topography (PAINT) with extended imaging durations that enables the study of antibody binding kinetics in the cellular environment. SMIM revealed heterogeneous binding kinetics and the effect of concentration and antibody valency on the association and dissociation rates on antibody-antigen interactions in their cellular environments. We thereby demonstrate SMIM as a versatile single-molecule technique for studying strong, transient biomolecular interactions.

Biotin−Avidin Binding Kinetics Measured by Single-Molecule Imaging

2009 ◽  
Vol 81 (1) ◽  
pp. 336-342 ◽  
Author(s):  
Joshua R. Wayment ◽  
Joel M. Harris
Keyword(s):  
Single Molecule ◽  
Binding Kinetics ◽  
Single Molecule Imaging
Sign in / Sign up

Export Citation Format

Share Document