scholarly journals Ekstraksi DNA total dari sumber jaringan hewan (Ikan Kerapu) menggunakan metode kit for animal tissue

2019 ◽  
Vol 3 (1) ◽  
pp. 40
Author(s):  
Yanti Ariyanti ◽  
Sister Sianturi
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Dna Analysis ◽  
Early Stage ◽  
Animal Tissue ◽  
High Yield ◽  
Tissue Samples ◽  
Organic Extraction ◽  
Cell Components ◽  
Total Dna

DNA extraction is a series of processes to separate DNA from other cell components. Extraction is the most crucial early stage in molecular research. There are two methods in DNA extraction: traditional organic extraction and non-organic adoption extraction method. Traditional organic extraction method are used by many laboratories to obtain amount of high-yield DNA. However, in recent years it has been a tendency to adopt non-organic commercial protocols, known as extraction kit. This method was relatively faster, low times-consuming and avoid the toxicity due to phenol use. This study aims to determine the steps of DNA extraction to obtain total DNA from muscle tissue of fish grouper using Extraction Kit for Animal Tissue. The Kit extraction method has an advantages that is the processing time is shorter, simple, non-toxic and high-purity DNA yields. A total of 4 tissue samples from grouper muscle were successfully extracted using an Extraction Kit that was indicated by the visualization of the band on qualitative DNA analysis in 1% agarose gel. Keywords: DNA extraction, extraction kit, high-yield DNA, molecular method

scholarly journals Direct Identification of Vibrio vulnificus in Clinical Specimens by Nested PCR

1998 ◽  
Vol 36 (10) ◽  
pp. 2887-2892 ◽  
Author(s):  
Shee Eun Lee ◽  
Soo Young Kim ◽  
Sei Jong Kim ◽  
Hyun Soo Kim ◽  
Jong Hee Shin ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Nested Pcr ◽  
Extraction Method ◽  
Vibrio Vulnificus ◽  
High Yield ◽  
Chromosomal Dna ◽  
Direct Identification ◽  
Clinical Specimens ◽  
Dna Extraction Method ◽  
Dna Fragment

This study was performed to establish optimal nested PCR conditions and a high-yield DNA extraction method for the direct identification ofVibrio vulnificus in clinical specimens. We designed two sets of primers targeting the V. vulnificushemolysin/cytolysin gene. The target of the first primer set (P1-P2; sense, 5′-GAC-TAT-CGC-ATC-AAC-AAC-CG-3′, and antisense, 5′-AGG-TAG-CGA-GTA-TTA-CTG-CC-3′, respectively) is a 704-bp DNA fragment. The second set (P3-P4; sense, 5′-GCT-ATT-TCA-CCG-CCG-CTC-AC-3′, and antisense, 5′-CCG-CAG-AGC-CGT-AAA-CCG-AA-3′, respectively) amplifies an internal 222-bp DNA fragment. We developed a direct DNA extraction method that involved boiling the specimen pellet in a 1 mM EDTA–0.5% Triton X-100 solution. The new DNA extraction method was more sensitive and reproducible than other conventional methods. The DNA extraction method guaranteed sensitivity as well, even when V. vulnificus cells were mixed with other bacteria such asEscherichia coli or Staphylococcus aureus. The nested PCR method could detect as little as 1 fg of chromosomal DNA and single CFU of V. vulnificus. We applied the nested PCR protocol to a total of 39 serum specimens and bulla aspirates from septicemic patients. Seventeen (94.4%) of the 18V. vulnificus culture-positive specimens were positive by the nested PCR. Eight (42.1%) of the 19 culture-negative samples gave positive nested PCR results.

scholarly journals Optimisation of an Automated DNA Extraction Method for Bone and Teeth Samples and Applicability to Two Forensic Cases

2021 ◽  
Vol 1 (3) ◽  
pp. 194-201
Author(s):  
Jasmin Zoranjic ◽  
Jasmine W. Tay ◽  
Nicholas S. Mountford ◽  
Marie S. Rye
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Disaster Victim Identification ◽  
Victim Identification ◽  
Dna Quality ◽  
Dna Extraction Method ◽  
Organic Extraction ◽  
Automated Dna Extraction ◽  
Dna Profiles ◽  
Processing Steps

Bones and teeth are highly challenging sources of DNA in forensic science and human remains identification, requiring multiple laborious processing steps. In this study, we compared an organic phenol–chloroform method to the QIAamp® DNA Investigator and PrepFiler Express BTA™ methods in order to identify the most efficient automated DNA extraction method for bones and teeth. Results from individual tooth powder replicates showed that the PrepFiler Express BTA™ method extracted the highest yields of DNA per mg of tooth powder, returning a minimum of 20/21 PowerPlex® 21 loci. Samples extracted using the organic extraction or QIAamp® DNA Investigator methods produced PowerPlex® 21 profiles displaying a ski-slope morphology. The improved DNA quality and yield from the PrepFiler Express BTA™ method was verified using aged samples, where higher DNA yields per mg of powder and more informative profiles were obtained. Furthermore, the PrepFiler Express BTA™ method subsequently provided useful DNA profiles for two forensic cases involving degraded bone samples. Overall, this study showed that the PrepFiler Express BTA™ chemistry is a reliable and robust method for DNA extraction from bone and teeth samples, and will allow larger numbers of samples to be efficiently extracted in the event of a Disaster Victim Identification event.

scholarly journals Validation of a universal DNA extraction method for human and microbiAL DNA analysis

2019 ◽  
Vol 7 (1) ◽  
pp. 256-258
Author(s):  
Federica Alessandrini ◽  
Andrea Brenciani ◽  
Simona Fioriti ◽  
Filomena Melchionda ◽  
Marina Mingoia ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Dna Analysis ◽  
Dna Extraction Method ◽  
Microbial Dna

scholarly journals New DNA Extraction Method for the Detection of Pneumocystis in Lung Tissue Samples of Colonized Individuals

OBM Genetics ◽  
2019 ◽  
Vol 3 (1) ◽  
pp. 1-1
Author(s):  
Susana Ruiz-Ruiz ◽  
◽  
Carolina A Ponce ◽  
Nicole Pesantes ◽  
Rebeca Bustamante ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Lung Tissue ◽  
Extraction Method ◽  
Tissue Samples ◽  
Dna Extraction Method

scholarly journals Novel kit for efficient extraction of long-segment genomic DNA by nanodisk adsorption

2019 ◽  
Vol 1 (1) ◽  
pp. 4-5
Author(s):  
Siqi Wu ◽  
Zhaofang Han ◽  
Hesong Qiu
Keyword(s):  
Molecular Biology ◽  
Dna Extraction ◽  
High Purity ◽  
Genomic Dna ◽  
Extraction Methods ◽  
Biochemical Properties ◽  
High Efficacy ◽  
Tissue Samples ◽  
Efficient Extraction ◽  
Total Dna

The acquisition of complete DNA is usually the first and most critical step in many basic molecular biology applications. Isolation of complete total DNA from tissue samples is related to different physical and biochemical properties of tissue. Long fragments of DNA are easily degraded and fragmented, which poses a challenge for extracting complete long fragments of DNA. By comparing several different extraction methods and verifying the concentration and length of extracted DNA, it is proved that the kit we used has high  efficacy. We provide tissue kit DNA extraction for long fragments and obtain long fragments of high purity DNA from almost any type of tissue, especially muscle and blood tissues.

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