SINTESIS ANALOG UK-3A : 6-HIDROKSI-N-FENILNIKOTINAMIDA DAN 6-HIDROKSI-N- FENILPIKOLINAMIDA DAN UJI SITOTOKSISITAS SECARA IN VITRO TERHADAPSEL KANKER MURINE LEUKEMIA P388

Viral membrane fusion proteins of class I are trimers in which the protomeric unit is a complex of a surface subunit (SU) and a fusion active transmembrane subunit (TM). Here we have studied how the protomeric units of Moloney murine leukemia virus envelope protein (Env) are activated in relation to each other, sequentially or simultaneously. We followed the isomerization of the SU-TM disulfide and subsequent SU release from Env with biochemical methods and found that this early activation step occurred sequentially in the three protomers, generating two asymmetric oligomer intermediates according to the scheme (SU-TM)3→ (SU-TM)2TM → (SU-TM)TM2→ TM3. This was the case both when activation was triggered in vitro by depleting stabilizing Ca2+from solubilized Env and when viral Env was receptor triggered on rat XC cells. In the latter case, the activation reaction was too fast for direct observation of the intermediates, but they could be caught by alkylation of the isomerization active thiol.

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Heterogeneity of Murine Leukemia Virus in vitro DNA; Detection of Viral DNA in Mammalian Cells

Science ◽

10.1126/science.172.3990.1353 ◽

1971 ◽

Vol 172 (3990) ◽

pp. 1353-1355 ◽

Cited By ~ 55

Author(s):

L. D. Gelb ◽

S. A. Aaronson ◽

M. A. Martin

Keyword(s):

Murine Leukemia Virus ◽

Mammalian Cells ◽

Dna Detection ◽

Leukemia Virus ◽

Viral Dna ◽

Murine Leukemia

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Strong selection for cells containing new ecotropic recombinant murine leukemia virus provirus after propagation of C57BL/6 radiation-induced thymoma cells in vitro or in vivo

Molecular and Cellular Biology ◽

10.1128/mcb.3.9.1675-1679.1983 ◽

1983 ◽

Vol 3 (9) ◽

pp. 1675-1679

Author(s):

P Jolicoeur ◽

E Rassart ◽

P Sankar-Mistry

Keyword(s):

Cell Lines ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Primary Radiation ◽

Secondary Tumors ◽

Radiation Induced ◽

Viral Sequences ◽

Murine Leukemia

Using the Southern procedure, we have studied the presence of ecotropic-specific murine leukemia viral sequences in genomic DNA isolated from primary X-ray-induced thymomas, from lymphoid cell lines established from them, or from secondary tumors passaged in vivo. We found that primary radiation-induced thymomas and infiltrated spleens do not harbor newly acquired ecotropic provirus. However, additional ecotropic proviruses (which appear recombinant in the gagpol region) could be detected in most of the tumorigenic cell lines established in vitro from them and in tumors arising from subcutaneous transplantation of the primary thymomas. These results suggest that primary radiation-induced thymomas may not be clonal. They also indicate a strong correlation between the presence of ecotropic recombinant proviruses in the genome and the growth ability, both in vitro and in vivo, of specific cells within these thymomas, suggesting a possible mitogenic function for murine leukemia virus.

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IN VITRO LYMPHOID CELL TRANSFORMATION BY ABELSON MURINE LEUKEMIA VIRUS

Animal Virology ◽

10.1016/b978-0-12-077350-3.50023-x ◽

1976 ◽

pp. 311-320 ◽

Cited By ~ 1

Author(s):

Naomi Rosenberg ◽

David Baltimore

Keyword(s):

Lymphoid Cell ◽

Murine Leukemia Virus ◽

Cell Transformation ◽

Leukemia Virus ◽

Abelson Murine Leukemia Virus ◽

Murine Leukemia

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Inhibition of Gross Murine Leukemia Virus Replication by Rifamycin SV and Certain of Its Derivatives in vitro

Intervirology ◽

10.1159/000149744 ◽

1974 ◽

Vol 3 (1-2) ◽

pp. 84-96 ◽

Cited By ~ 6

Author(s):

William M. Shannon ◽

Louise Westbrook ◽

Frank M. Schabel, jr.

Keyword(s):

Virus Replication ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Rifamycin Sv ◽

Murine Leukemia

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Solanum lyratumExtracts Induce Extrinsic and Intrinsic Pathways of Apoptosis in WEHI-3 Murine Leukemia Cells and Inhibit Allograft Tumor

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/254960 ◽

2012 ◽

Vol 2012 ◽

pp. 1-13 ◽

Cited By ~ 17

Author(s):

Jai-Sing Yang ◽

Chia-Chun Wu ◽

Chao-Lin Kuo ◽

Yu-Hsuan Lan ◽

Chin-Chung Yeh ◽

...

Keyword(s):

Antitumor Activity ◽

Molecular Mechanisms ◽

Fas Ligand ◽

Leukemia Cells ◽

Cycle Arrest ◽

Phase Arrest ◽

Apoptotic Pathways ◽

Murine Leukemia

We investigated the molecular mechanisms of cell cycle arrest and apoptotic death induced bySolanum lyratumextracts (SLE) or diosgenin in WEHI-3 murine leukemia cellsin vitroand antitumor activityin vivo. Diosgenin is one of the components of SLE. Our study showed that SLE and diosgenin decreased the viable WEHI-3 cells and inducedG0/G1phase arrest and apoptosis in concentration- or time-dependent manners. Both reagents increased the levels of ROS production and decreased the mitochondrial membrane potential (ΔΨm). SLE- and diosgenin-triggered apoptosis is mediated through modulating the extrinsic and intrinsic signaling pathways. Intriguingly, the p53 inhibitor (pifithrin-α), anti-Fas ligand (FasL) mAb, and specific inhibitors of caspase-8 (z-IETD-fmk), caspase-9 (z-LEHD-fmk), and caspase-3 (z-DEVD-fmk) blocked SLE- and diosgenin-reduced cell viability of WEHI-3 cells. Thein vivostudy demonstrated that SLE has marked antitumor efficacy against tumors in the WEHI-3 cell allograft model. In conclusion, SLE- and diosgenin-inducedG0/G1phase arrest and triggered extrinsic and intrinsic apoptotic pathways via p53 activation in WEHI-3 cells. SLE also exhibited antitumor activityin vivo. Our findings showed that SLE may be potentially efficacious in the treatment of leukemia in the future.

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