THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS

Agriciltural microbiology ◽

10.35868/1997-3004.24.79-82 ◽

2016 ◽

Vol 24 ◽

pp. 79-82

Author(s):

H. M. Shevaha ◽

M. M. Kyryk ◽

V. M. Hunchak ◽

T. M. Oliinyk

Keyword(s):

Growth And Development ◽

Culture Medium ◽

In Vitro Growth ◽

Maize Starch ◽

Potato Plants

Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and development of potato springs.

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Source for Gloxinia (Sinningia Spp.) and Potato (Solanum Tuberosum) Micropropagation

ASEAN Journal on Science and Technology for Development ◽

10.29037/ajstd.123 ◽

2017 ◽

Vol 23 (4) ◽

pp. 333

Author(s):

Duong Tan Nhut ◽

Mai Thi Ngoc Huong

Keyword(s):

Solanum Tuberosum ◽

Growth And Development ◽

In Vitro Growth ◽

Lighting System ◽

Potato Plants ◽

Light Intensities ◽

Lighting Systems ◽

Potato Plantlets ◽

Better Than

An efficient lighting system (Compact 3U) was successfully applied to the micropropagation of gloxinia and potato plants. To compare the in vitro growth of plantlets under Neon and Compact 3U lighting systems, gloxinia and potato shoots were cultured on suitable media at different light intensities: (1) 45 µmol m-2s-1 (under Neon lighting system as a control); (2) 45 µmol m-2s-1, (3) 60µmol m-2s-1, and (4) 75 µmol m-2s-1 (under Compact 3U lighting systems). The results obtained after 3 weeks of in vitro culture and 6 weeks of acclimatization in a greenhouse showed that the growth of plantlets cultured under the Compact 3U system was better than that under Neon lighting system. The Compact 3U lighting source had a highly significant effect on the growth and development of gloxinia and potato plantlets. Besides, the data in our study also indicated that gloxinia and potato plantlets adapted differently to different lighting sources and light intensities.

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Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

Ciência Rural ◽

10.1590/0103-8478cr20160977 ◽

2018 ◽

Vol 48 (7) ◽

Author(s):

Renato Fernandes Galdiano Júnior ◽

Cibele Mantovani ◽

Eliana Gertrudes de Macedo Lemos

Keyword(s):

Survival Data ◽

Culture Medium ◽

Seedling Survival ◽

In Vitro Growth ◽

Growth Data ◽

Carbohydrate Source ◽

Carbohydrate Supplementation ◽

Percentage Survival ◽

Half Strength

ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.

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In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632021v5167131 ◽

2021 ◽

Vol 51 ◽

Author(s):

Kaliana Gottschalk de Freitas ◽

José Carlos Sorgato ◽

Jackeline Schultz Soares ◽

Luan Marlon Ribeiro

Keyword(s):

Plant Height ◽

Natural Ventilation ◽

Culture Medium ◽

Culture Media ◽

Ventilation System ◽

Light Condition ◽

Leaf Length ◽

In Vitro Growth ◽

Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

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