scholarly journals PRODUCTION OF PHYTOHORMONES OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE UNDER THEIR SIMULTANEOUS CULTIVATION

2018 ◽  
Vol 28 ◽  
pp. 33-40
Author(s):  
S.F. Kozar
Keyword(s):  
Abscisic Acid ◽  
Mixed Culture ◽  
Pure Culture ◽  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Culture Fluid ◽  
Culture Liquid ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Pure Cultures

Objective. Investigate the activity of biosynthesis of phytohormonal substances with nitrogen-fixing bacteria Bradyrhizobium japonicum and Azospirillum brasilense in pure and mixed culture. Methods. Microbiological, chromatographic, and mathematical. Results It has been established that the simultaneous cultivation of B. japonicum M-8 and A. brasilense 410 increases the content of gibberellins and cytokinins in the culture fluid of the test microorganisms. The content of gibberellic acid and isopentenylidene has increased most intensively in mixed culture compared with the pure culture of rhizobia. In the course of co-cultivation, the studied diazotrophs more intensively produced auxins compared to soybean rhizobia in pure culture, but less compared to pure culture of azospirilla. The highest level of abscisic acid that can inhibit the formation of nodules was found in A. brasilense 410 culture fluid, and it was lower when cultivating B japonicum M-8. However, the smallest amount of this phytohormone was found in the culture liquid of diazotrophs under their co-cultivation. The lowest ratio of auxin/cytokinin was found in B. japonicum M-8 and A. brasilense 410 culture fluid under their co-cultivation, which should positively influence the formation of a symbiotic system when interacting with soybean plants. Conclusion. A combination of cultivating rhizobia and azospirilla showed an increase in the amount of cytokinins and gibberellins in the culture fluid of the microorganisms, a decrease in the amount of abscisic acid and improvement in the auxin/cytokinin ratio compared to the values of the pure cultures of the nitrogen-fixing bacteria studied. An analysis of the quantitative parameters of the content of phytohormones suggests the feasibility of combining B. japonicum and A. brasilense in a mixed culture for the effective introduction of rhizobia in soybean agrocenosis.

scholarly journals THE INFLUENCE OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE ON THE VERTICAL MIGRATION OF NUTRIENTS AT SOYBEAN CULTIVATION

2015 ◽  
Vol 21 ◽  
pp. 39-43
Author(s):  
S. F. Kozar ◽  
I. M. Pyschur ◽  
V. M. Nesterenko
Keyword(s):  
Chlorophyll Content ◽  
Vertical Migration ◽  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Water Soluble ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Joint Application ◽  
Calcium And Magnesium ◽  
Soybean Plants

The paper presents the research results of pre-sowing seeds bacterization with nitrogen fixing bacteria Bradyrhizobium japonicum and Azospirillum brasilense influence on the loss of moisture, water soluble humus and nutrients. It was shown that seeds bacterization reduces the leaching intensity of nitrates, phosphorus, potassium, calcium and magnesium. Moreover, the least losses of nutrients were observed in a variants with joint application of both studied nitrogen fixing microorganisms. It was noted that seeds bacterization with B. japonicum and A. brasilense had promoted increase of chlorophyll content in the leaves of soybean plants. The highest yield was observed in the variant with the joint use of rhizobia and azospirillum.

scholarly journals ECONOMIC AND ENERGETIC EFFICIENCY OF COMPLEX BACTERIZATION IN SOYBEAN CULTIVATION

2016 ◽  
Vol 23 ◽  
pp. 24-29
Author(s):  
Y. M. Khalep ◽  
S. F. Kozar
Keyword(s):  
Pure Culture ◽  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Positive Impact ◽  
Mixed Cultures ◽  
Energetic Efficiency ◽  
Nitrogen Fixing

The results of efficiency studies by major economic and energetic parameters of pretreatment of soybean with inoculants based on rhizobia Bradyrhizobium japonicum and associative diazotrophs Azospirillum brasilense are provided. It was established that the highest levels of the studied parameters are achieved using mixed cultures of nitrogen fixing microorganisms. The high economic and energetic efficiency was also observed during inoculation of soybean with rhizobia pure culture. However, no significant positive impact of A. brasilense applied separately was found in terms of studied parameters.

Polyhydroxyalkanoate analysis inAzospirillum brasilense

1995 ◽  
Vol 41 (13) ◽  
pp. 73-76 ◽  
Author(s):  
Robin Itzigsohn ◽  
Oded Yarden ◽  
Yaacov Okon
Keyword(s):  
Azospirillum Brasilense ◽  
Bacterial Species ◽  
Carbon Sources ◽  
Dry Mass ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Qualitative And Quantitative ◽  
Alkanoic Acids ◽  
Quantitative Analyses

The considerable industrial interest in the qualitative and quantitative production of polyhydroxyalkanoates in microorganisms has led to the characterization of those synthesized in the nitrogen-fixing bacteria Azospirillum brasilense and Azotobacter paspali. In contrast to some other bacterial species, Azospirillum brasilense does not produce copolymers of hydroxyalkanoates when grown under the different carbon sources assayed, namely n-alkanoic acids, hydroxyalkanoates, and sugars with varying C:N ratios. Rather, only homopolymers of polyhydroxybutyrate were detected, comprising up to 70% of the cell dry mass. No copolymers were detected in Azotobacter paspali. Quantitative analyses of poly(β-hydroxybutyrate) are also presented.Key words: Azospirillum spp., Azotobacter paspali, polyhydroxyalkanoate analysis, PHA, PHB.

scholarly journals Enhanced Removal of a Pesticides Mixture by Single Cultures and Consortia of Free and ImmobilizedStreptomycesStrains

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
María S. Fuentes ◽  
Gabriela E. Briceño ◽  
Juliana M. Saez ◽  
Claudia S. Benimeli ◽  
María C. Diez ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Pure Culture ◽  
Immobilized Cells ◽  
Mixed Cultures ◽  
Ex Situ ◽  
Antagonistic Effects ◽  
Pure Cultures

Pesticides are normally used to control specific pests and to increase the productivity in crops; as a result, soils are contaminated with mixtures of pesticides. In this work, the ability ofStreptomycesstrains (either as pure or mixed cultures) to remove pentachlorophenol and chlorpyrifos was studied. The antagonism among the strains and their tolerance to the toxic mixture was evaluated. Results revealed that the strains did not have any antagonistic effects and showed tolerance against the pesticides mixture. In fact, the growth of mixed cultures was significantly higher than in pure cultures. Moreover, a pure culture (Streptomycessp. A5) and a quadruple culture had the highest pentachlorophenol removal percentages (10.6% and 10.1%, resp.), whileStreptomycessp. M7 presented the best chlorpyrifos removal (99.2%). Mixed culture of allStreptomycesspp. when assayed either as free or immobilized cells showed chlorpyrifos removal percentages of 40.17% and 71.05%, respectively, and for pentachlorophenol 5.24% and 14.72%, respectively, suggesting better removal of both pesticides by using immobilized cells. These results reveal that environments contaminated with mixtures of xenobiotics could be successfully cleaned up by using either free or immobilized cultures ofStreptomyces, throughin situorex situremediation techniques.

Isolation and taxonomy of the blue-green algae (Cyanobacteria), Nostoc and Anabaena in Kerala State, India

2020 ◽  
Vol 62 (1-2) ◽  
pp. 163-174
Author(s):  
T. S. Thilak ◽  
P. V. Madhusoodanan ◽  
N. S. Pradeep ◽  
R. Prakashkumar
Keyword(s):  
Green Algae ◽  
Pure Culture ◽  
Paddy Fields ◽  
Atmospheric Nitrogen ◽  
Nitrogen Fixing ◽  
Blue Green Algae ◽  
Heterocystous Cyanobacteria ◽  
Pure Cultures ◽  
Photosynthetic Microorganisms ◽  
Kerala State

Blue-green algae (also called cyanobacteria) are ubiquitous, pristine and pioneer photosynthetic microorganisms. Many species of cyanobacteria are capable of fixing atmospheric nitrogen and such species in wet soils are simultaneously augmenting the fertility of the soil, acting as natural bio-fertilizers. Nostoc and Anabaena are the two important genera of heterocystous cyanobacteria capable of contributing nitrogen to soil, especially in paddy fields. The major objectives of the investigation included survey, collection, isolation and pure culture of nitrogen-fixing species of Cyanobacteria in the soils of Kerala state, India. Altogether, pure cultures of 12 species of Nostoc and 5 species of Anabaena are prepared.

scholarly journals Efficiency and response of corn cultivars to nitrogen, associated or not with Azospirillum brasilense

2021 ◽  
Vol 8 (4) ◽  
pp. 217-224
Author(s):  
Vanderlan Carneiro Dias ◽  
Joênes Mucci Peluzio ◽  
Flávio Sérgio Afférri ◽  
Maria Dilma de Lima ◽  
Domingos Bonfim Ribeiro dos Santos ◽  
...  
Keyword(s):  
Experimental Design ◽  
Azospirillum Brasilense ◽  
Variance Analysis ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Nitrogen Use ◽  
Maize Cultivars ◽  
Seed Inoculation

Green corn is an economic and social alternative for small and medium-sized farmers. However, the high cost of the insums, especially in relation to the use of nitrogen, has advanced studies with nitrogen-fixing bacteria. This study evaluated commercial maize cultivars for the production of green ears, responsive and efficient to nitrogen use, with and without application in Azospirillum brasilense seeds. Two trials were installed in the agricultural years 2019/20 and 2020/21 in soweds carried out on 12/04/2019 and 10/12/2020 in this order, at the Federal University of Tocantins, Palmas-TO. In each trial, the experimental design was randomized blocks, with three replications, being the treatments arranged in subdivided plots, where the treatments involving the processes with inoculation of seeds with the bacterium Azospirillum (C Az) and without inoculation of seeds (S Az) were allocated in the plots, two doses of nitrogen (30 and 120 kg ha-1 N, considered as low and high N, respectively) and in the subplots eight corn cultivars were allocated in the subplots. Joint variance analysis was performed for each of the seed inoculation processes (C Az and S Az) and then the efficiency and response of corn cultivars were studied for the use of N for each process. The inoculation of seeds with the bacterium promoted changes in the efficiency and response of cultivars regarding the use of nitrogen. The cultivar BRS-3046 was the most important for the cultivation of green ears, since it was efficient and responsive to the use of nitrogen with Azospirillum brasilense.

scholarly journals Potential Role of Thiobacillus caldus in Arsenopyrite Bioleaching

1999 ◽  
Vol 65 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Mark Dopson ◽  
E. Börje Lindström
Keyword(s):  
Mixed Culture ◽  
Pure Culture ◽  
Potential Role ◽  
Mixotrophic Growth ◽  
Moderately Thermophilic ◽  
Surface Active Agents ◽  
Pure Cultures ◽  
Surface Active ◽  
Leaching Efficiency

ABSTRACT We investigated the potential role of the three strains ofThiobacillus caldus (KU, BC13, and C-SH12) in arsenopyrite leaching in combination with a moderately thermophilic iron oxidizer,Sulfobacillus thermosulfidooxidans. Pure cultures ofT. caldus and S. thermosulfidooxidans were used as well as defined mixed cultures. By measuring released iron, tetrathionate, and sulfur concentrations, we found that the presence ofT. caldus KU and BC13 in the defined mixed culture lowered the concentration of sulfur, and levels of tetrathionate were comparable to or lower than those in the presence of S. thermosulfidooxidans. This suggests that T. caldusgrows on the sulfur compounds that build up during leaching, increasing the arsenopyrite-leaching efficiency. This result was similar to leaching arsenopyrite with a pure culture of S. thermosulfidooxidans in the presence of yeast extract. Therefore, three possible roles of T. caldus in the leaching environment can be hypothesized: to remove the buildup of solid sulfur that can cause an inhibitory layer on the surface of the mineral, to aid heterotrophic and mixotrophic growth by the release of organic chemicals, and to solubilize solid sulfur by the production of surface-active agents. The results showed that T. caldus KU was the most efficient at leaching arsenopyrite under the conditions tested, followed by BC13, and finally C-SH12.

scholarly journals Technical sheet of influence of Freeze-Dried Yeast Starter Cultures on Volatile Compounds of Tchapalo, a Traditional Sorghum Beer from Côte d’Ivoire

2017 ◽  
Vol 6 (3) ◽  
pp. 913-918
Author(s):  
COULIBALY Wahauwouélé Hermann ◽  
BOUATENIN Koffi Maizan Jean-Paul ◽  
KOUAME Kohi Alfred ◽  
RIGOU Peggy
Keyword(s):  
Volatile Compounds ◽  
Mixed Culture ◽  
Pure Culture ◽  
Empirical Process ◽  
Starter Cultures ◽  
Higher Alcohols ◽  
Higher Alcohol ◽  
Freeze Dried ◽  
Pure Cultures ◽  
Microbiological Stability

The production of the Ivorian sorghum beer known as tchapalo remains more or less an empirical process. The use of starter cultures was therefore suggested as the appropriate approach to alleviate the problems of variations inorganoleptic quality and microbiological stability. In this study, we evaluated the capacity of S. cerevisiae and C. tropicalis to produce sorghum beer as freeze-dried starter in mixed or pure cultures. Beers produced with mixed freeze-dried cultures of S. cerevisiae F12-7 and C. tropicalis C0-7 showed residual sugars and ethanol contents similar to beers obtained with S. cerevisiae F12-7 pure culture, but the total sum of organic acids analyzed was the highest with the mixed culture (15.71 g/L). Higher alcohols were quantitatively the largest group of volatile compounds detected in beers. Among these compounds, 2-phenyl ethanol, a higher alcohol that plays an important role in beer flavor, was highly produced with the mixed culture (10174.8 µg/L) than with the pure culture (8749.9 µg/L).

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