scholarly journals ANATOMY AND HISTOPATHOLOGY OF ALIMENTARY CANAL OF HALYS DENTATUS (HEMIPTERA: PENTATOMIDAE) TREATED.

2019 ◽  
pp. 1-6
Author(s):  
Jyoti H. Gangurde ◽  
Sachin A. Gurule* ◽  
Savita K. Tidame

Halys dentatus is well known sap sucker living under the bark and having the reputation of affecting nutritional supply of their host plants. Dichlorovos (2,2-dichlorovinyl dimethyl phosphate) is an organo-phosphorus compound previously used for controlling crawling and flying insect pests. In the present study dichlorovos has been tested as systemic poison for checking its lethality against plant bug Halys dentatus. The oral application has found to be effective, as it disintegrates components of gastro-intestinal tract affecting vitality of test individuals. The adult plant bug when treated with dichlorovos, alteration in histological structures of ventriculus has been observed. In most of the cases alteration in shape of ventricular components has been noticed; basement membrane has found to be collapse while longitudinal and circular muscles layers were also disrupted. The cell boundaries of epithelial cells found to be busted and secretory cells become denatured; nuclei were found to be stained intensely as compared with untreated histological sections

2019 ◽  
pp. 1-6
Author(s):  
Jyoti H. Gangurde ◽  
Sachin A. Gurule* ◽  
Savita K. Tidame

Halys dentatus is well known sap sucker living under the bark and having the reputation of affecting nutritional supply of their host plants. Dichlorovos (2,2-dichlorovinyl dimethyl phosphate) is an organo-phosphorus compound previously used for controlling crawling and flying insect pests. In the present study dichlorovos has been tested as systemic poison for checking its lethality against plant bug Halys dentatus. The oral application has found to be effective, as it disintegrates components of gastro-intestinal tract affecting vitality of test individuals. The adult plant bug when treated with dichlorovos, alteration in histological structures of ventriculus has been observed. In most of the cases alteration in shape of ventricular components has been noticed; basement membrane has found to be collapse while longitudinal and circular muscles layers were also disrupted. The cell boundaries of epithelial cells found to be busted and secretory cells become denatured; nuclei were found to be stained intensely as compared with untreated histological sections


1874 ◽  
Vol 22 (148-155) ◽  
pp. 293-294 ◽  

The following results relating to the anatomy of the mucous membrane of the alimentary canal were obtained in the laboratory of the Brown Institution. The researches were carried out under the direction of Dr. Klein. 1. Connective-tissue corpuscles amongst the epithelium .—In specimens hardened in chromic acid and alcohol and stained in hæmatoxylin, structures are constantly seen among the columnar epithelium of the intestinal tract in many animals (as monkey, sheep, cat, dog, rat, rabbit) which belong to the connective tissue. These are :— (1) a delicate reticulum, which is continuous with that formed by the most superficial layer of connective-tissue corpuscles (the basement membrane) ; (2) round nucleated cells, exactly similar to those of the mucosa.


2014 ◽  
Vol 9 (3) ◽  
pp. 196-204 ◽  
Author(s):  
Lucia Montenegro ◽  
Giuseppe Losurdo ◽  
Raffaele Licinio ◽  
Maria Zamparella ◽  
Floriana Giorgio ◽  
...  

2021 ◽  
Vol 9 (5) ◽  
pp. 1013
Author(s):  
Karina Arellano-Ayala ◽  
Juhwan Lim ◽  
Subin Yeo ◽  
Jorge Enrique Vazquez Bucheli ◽  
Svetoslav Dimitrov Todorov ◽  
...  

Preservation of probiotics by lyophilization is considered a method of choice for developing stable products. However, both direct consumption and reconstitution of dehydrated probiotic preparations before application “compromise” the survival and functional characteristics of the microorganisms under the stress of the upper gastro-intestinal tract. We evaluated the impact of different food additives on the viability, mucin adhesion, and zeta potential of a freeze-dried putative probiotic, Lactiplantibacillus (Lp.) plantarum HAC03. HAC03-compatible ingredients for the formulation of ten rehydration mixtures could be selected. Elevated efficacy was achieved by the B-active formulation, a mixture of non-protein nitrogen compounds, sugars, and salts. The survival of Lp. plantarum HAC03 increased by 36.36% compared rehydration with distilled water (4.92%) after passing simulated gastro-intestinal stress conditions. Cell viability determined by plate counting was confirmed by flow cytometry. B-active formulation also influenced Lp. plantarum HAC03 functionality by increasing its adherence to a Caco-2 cell-line and by changing the bacterial surface charge, measured as zeta potential.Hydrophobicity, mucin adhesion and immunomodulatory properties of Lp. plantarum HAC03 were not affected by the B-active formulation. The rehydration medium also effectively protected Lp. plantarum ATCC14917, Lp. plantarum 299v, Latilactobacillus sakei (Lt.) HAC11, Lacticaseibacillus (Lc.) paracasei 532, Enterococcus faecium 200, and Lc. rhamnosus BFE5263.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1824
Author(s):  
Giorgia Corazzola ◽  
Matteo Baini ◽  
Carla Grattarola ◽  
Cristina Panti ◽  
Federica Marcer ◽  
...  

Organs and content of the gastro-intestinal tract (GIT) of marine mammals are relevant for a variety of investigations and provide data to researchers from different fields. Currently used protocols applied to the GIT for specific analysis limit the possibility to execute other investigations and important information could be lost. To ensure a proper sample collection and a multidisciplinary investigation of the GIT of marine mammals, a new multi-sieves tool and a specific protocol have been developed. This new device and approach allowed the simultaneous sampling of the GIT and its content for the main investigations concerned. The samples collected during these preliminary trials were suitable to perform all the different research procedures considered in this work. The obtained results show that with a few and easy procedural adjustments, a multidisciplinary sampling and evaluation of the GIT of marine mammals is possible. This will reduce the risk of losing important data aimed at understanding the cause of death of the animal, but also biology and ecology of marine mammals, and other important data for their conservation and habitats management.


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