“IN VITRO STUDY OF HOMOEOPATHIC MEDICINE SARSAPARILLA Q, 6C, 12C, 30C, 200C, 1M AS AN INHIBITOR OF CALCIUM OXALATE AND CALCIUMPHOSPHATE CRYSTALLISATION.”

Background: Around 12% of world population has been infected by renal stone disease which has multiphase etiological factors with high recurrence rate. Thus, in order to reduce the recurrence rate, use of homoeopathic intervention can be the most effective alternative option and Sarsaparilla is one of the frequently prescribed medicines by homoeopath in renal stones. Aim: To nd out the inhibitory action of homoeopathic medicine Sarsaparilla Q, 6C, 12C, 30C, 200C, 1M on calcium oxalate and calcium phosphate crystallization by in vitro study. Methodology: In vitro crystallisation of calcium oxalate and calcium phosphate was carried out to evaluate effectiveness of Homoeopathic Medicine Sarsaparilla as an inhibitor. Calcium Oxalate crystallisation assay was experimented by using JASCO-UV/VISIBLE-630 Spectrophotometer. Slope of Nucleation and Aggregation phases is calculated using linear regression analysis and percentage of inhibition was calculated. Calcium phosphate crystallisation inhibition by Sarsaparilla was calculated by measuring concentration of calcium and phosphate ions, using Digital Photo colorimeter by Trinder and Gomeri method respectively. Antioxidant activity of Sarsaparilla Mother Tincture and potencies was measured by DPPH free radical scavenging assay by spectrophotometer. Result: Maximum inhibition of calcium oxalate crystallisation has been noticed in Sarsaparilla Mother Tincture and 1M potency. While Sarsaparilla 30C showing maximum inhibition of Calcium Phosphate crystallisation. Sarsaparilla mother tincture was able to decolorize during DPPH assay in which Inhibition % was decreased as potency increased (Q>30C>1M). Conclusion: The present in vitro study has shown potential role of Homoeopathic medicine Sarsaparilla as an inhibitor of calcium oxalate and calcium phosphate crystallisation.

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Is it possible to regain lost minerals to initial enamel lesions using Enamel Matrix Derivatives combined with Casein Phosphopeptide Amorphous Calcium Phosphate: an in vitro study

European Journal of Therapeutics ◽

10.5152/eurjther.2019.190110 ◽

2019 ◽

Author(s):

Gul Keskin ◽

◽

Cigdem Guler ◽

Sibel Yildirim ◽

◽

...

Keyword(s):

Calcium Phosphate ◽

Amorphous Calcium Phosphate ◽

In Vitro Study ◽

Vitro Study ◽

Enamel Matrix ◽

Enamel Matrix Derivatives ◽

Matrix Derivatives ◽

Casein Phosphopeptide

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“EFFECT OF HOMOEOPATHIC MEDICINE TRIBULUS TERRESTRIS Q, 6C, 12C, 30C, 200C, 1M AS AN INHIBITOR OF CALCIUM OXALATE AND CALCIUM PHOSPHATE CRYSTALLIZATION IN VITRO STUDY”

10.36106/ijsr/3404116 ◽

2021 ◽

pp. 45-48

Author(s):

Sharada Shankar Gowda ◽

Tanveer A. Khan ◽

Ajay Namdeo ◽

Chetan H. Shinde

Keyword(s):

Antioxidant Activity ◽

Calcium Phosphate ◽

Calcium Oxalate ◽

Radical Scavenging ◽

Medical Attention ◽

Tribulus Terrestris ◽

Vitro Experiment ◽

In Vitro Experiment ◽

Maximum Inhibition

Background: Urolithiasis is one of the common conditions in the society and it needs medical attention due to its increase in prevalence. The use of the homeopathic medicines has found to be,of great importance in the treatment of urolithiasis and certainly homoeopathy is a promising eld in this condition. Objectives: The aim of this study was to evaluate the mechanism of urolithiasis and the inhibitory action of homeopathic drug Tribulus terrestris by in vitro experiment. Materials and Method: Homoeopathic preparation of Tribulus terrestris Q, 6C, 12C, 30C, 200C, 1M was planned to evaluate in vitro calcium oxalate and calcium phosphate crystallization using spectro-photometric and colorimetric assay respectively. Considering the role of reactive oxygen species as one of the etiological factors in stone formation, effective antioxidant activity of Tribulus terrestris was also performed by 2,2- diphenyl -1-picrylhydrazyl (DPPH) free radical scavenging assay. Result: Tribulus terrestris Q, 200C and 1M exerted maximum inhibition as 33.62%, 23.89% and 23.00% respectively to calcium oxalate nucleation assay whereas, Tribulus terrestris Q, 6C, 12C, 30C, 200C, 1M exerted maximum inhibition to calcium oxalate aggregation assay up to 76.19%. Tribulus terrestris 12C and 30C showed maximum inhibition as 82.28% and 16. 21% to calcium and phosphate ions respectively. Presence of antioxidant activity by DPPH radical assay for Tribulus terrestris Q and 12C which showed percentage inhibition as 33.11% and 0.95% respectively. Conclusions:Homoeopathic preparation Tribulus terrestris has potential effect on inhibition of calcium oxalate and calcium phosphate crystallization and also homoeopathic preparation of Tribulus terrestris is capable of showing presence of phytochemicals; anti-oxidant activity when performed by in vitro experiment.

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