“EFFECT OF HOMOEOPATHIC MEDICINE TRIBULUS TERRESTRIS Q, 6C, 12C, 30C, 200C, 1M AS AN INHIBITOR OF CALCIUM OXALATE AND CALCIUM PHOSPHATE CRYSTALLIZATION IN VITRO STUDY”

2021 ◽  
pp. 45-48
Author(s):  
Sharada Shankar Gowda ◽  
Tanveer A. Khan ◽  
Ajay Namdeo ◽  
Chetan H. Shinde
Keyword(s):  
Antioxidant Activity ◽  
Calcium Phosphate ◽  
Calcium Oxalate ◽  
Radical Scavenging ◽  
Medical Attention ◽  
Tribulus Terrestris ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Maximum Inhibition

Background: Urolithiasis is one of the common conditions in the society and it needs medical attention due to its increase in prevalence. The use of the homeopathic medicines has found to be,of great importance in the treatment of urolithiasis and certainly homoeopathy is a promising eld in this condition. Objectives: The aim of this study was to evaluate the mechanism of urolithiasis and the inhibitory action of homeopathic drug Tribulus terrestris by in vitro experiment. Materials and Method: Homoeopathic preparation of Tribulus terrestris Q, 6C, 12C, 30C, 200C, 1M was planned to evaluate in vitro calcium oxalate and calcium phosphate crystallization using spectro-photometric and colorimetric assay respectively. Considering the role of reactive oxygen species as one of the etiological factors in stone formation, effective antioxidant activity of Tribulus terrestris was also performed by 2,2- diphenyl -1-picrylhydrazyl (DPPH) free radical scavenging assay. Result: Tribulus terrestris Q, 200C and 1M exerted maximum inhibition as 33.62%, 23.89% and 23.00% respectively to calcium oxalate nucleation assay whereas, Tribulus terrestris Q, 6C, 12C, 30C, 200C, 1M exerted maximum inhibition to calcium oxalate aggregation assay up to 76.19%. Tribulus terrestris 12C and 30C showed maximum inhibition as 82.28% and 16. 21% to calcium and phosphate ions respectively. Presence of antioxidant activity by DPPH radical assay for Tribulus terrestris Q and 12C which showed percentage inhibition as 33.11% and 0.95% respectively. Conclusions:Homoeopathic preparation Tribulus terrestris has potential effect on inhibition of calcium oxalate and calcium phosphate crystallization and also homoeopathic preparation of Tribulus terrestris is capable of showing presence of phytochemicals; anti-oxidant activity when performed by in vitro experiment.

scholarly journals Effect of Dolichos biflorus Seeds Based Functional Beverage on In Vitro Calcium Oxalate Crystallization in Human Urine

2021 ◽  
Vol 12 (5) ◽  
pp. 5836-5844
Keyword(s):  
Antioxidant Activity ◽  
Calcium Oxalate ◽  
Human Urine ◽  
Crystallization Process ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Calcium Oxalate Crystals ◽  
Dolichos Biflorus ◽  
Oxalate Crystals

Calcium oxalate is the most common type of urolithiasis. The crystallization process includes nucleation, growth, and the aggregation of crystals. This study has used Dolichos biflorus seeds as a functional beverage to explore the role of its bioactive substances on the crystallization process of calcium oxalate in managing urolithiasis. A human urine model of in vitro calcium oxalate crystals was used in the study. Phytochemical screening of Functional beverage of Dolichos biflorus seeds was performed, and antioxidant activity was evaluated by measuring DPPH radical-scavenging activity, reducing power assay, and Hydrogen peroxide scavenging activity. Functional beverage of Dolichos biflorus seeds inhibited crystallization process by reducing aggregation of calcium oxalate crystals. The reduction in crystals aggregation helps prevent urolithiasis by keeping the crystals dispersed in the urine, controlling their size, and facilitating expulsion from the urinary tract. The results showed that the Functional beverage of Dolichos biflorus seeds has a significant quantity of flavonoids, glycosides, etc., and also possesses a significant antioxidant activity as evaluated by employing different antioxidant assays. Therefore, our findings suggested that the functional beverage of Dolichos biflorus seeds exhibited antiurolithiatic activity through inhibition of the crystallization process of the calcium oxalate process and significant antioxidant potential.

Comparative efficacy of antioxidant drugs in an in vitro experiment

2021 ◽  
Vol 2021 (2) ◽  
pp. 37-40
Author(s):  
Kseniya Smirnova ◽  
Dmitriy Gildikov
Keyword(s):  
Antioxidant Activity ◽  
Test System ◽  
Comparative Efficacy ◽  
Chemiluminescence Method ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Oxidation System

The aim of this work was to study the antioxidant activity of 3-hydroxypyridine derivatives: Mexidol-Vet® and Emycidin in a model oxidation system. Using the chemiluminescence method, it was found that the introduction of Mexidol-Vet® into the test system at a dose of 1 mg reliably suppresses the amplitude of a fast flash and decreases the formation of photons (light sum value), compared with the test system / placebo and the test system / Emicidin. The antioxidant activity of Mexidol-Vet® is significantly higher than Emicidin by 28.1%.

“IN VITRO STUDY OF HOMOEOPATHIC MEDICINE SARSAPARILLA Q, 6C, 12C, 30C, 200C, 1M AS AN INHIBITOR OF CALCIUM OXALATE AND CALCIUMPHOSPHATE CRYSTALLISATION.”

2021 ◽  
pp. 73-76
Author(s):  
Mugdhakumari R. Patel ◽  
Tanveer A. Khan ◽  
Ajay Namdeo ◽  
Chetan H. Shinde
Keyword(s):  
Calcium Phosphate ◽  
Calcium Oxalate ◽  
Recurrence Rate ◽  
Linear Regression Analysis ◽  
In Vitro Study ◽  
Vitro Study ◽  
World Population ◽  
Maximum Inhibition ◽  
Mother Tincture

Background: Around 12% of world population has been infected by renal stone disease which has multiphase etiological factors with high recurrence rate. Thus, in order to reduce the recurrence rate, use of homoeopathic intervention can be the most effective alternative option and Sarsaparilla is one of the frequently prescribed medicines by homoeopath in renal stones. Aim: To nd out the inhibitory action of homoeopathic medicine Sarsaparilla Q, 6C, 12C, 30C, 200C, 1M on calcium oxalate and calcium phosphate crystallization by in vitro study. Methodology: In vitro crystallisation of calcium oxalate and calcium phosphate was carried out to evaluate effectiveness of Homoeopathic Medicine Sarsaparilla as an inhibitor. Calcium Oxalate crystallisation assay was experimented by using JASCO-UV/VISIBLE-630 Spectrophotometer. Slope of Nucleation and Aggregation phases is calculated using linear regression analysis and percentage of inhibition was calculated. Calcium phosphate crystallisation inhibition by Sarsaparilla was calculated by measuring concentration of calcium and phosphate ions, using Digital Photo colorimeter by Trinder and Gomeri method respectively. Antioxidant activity of Sarsaparilla Mother Tincture and potencies was measured by DPPH free radical scavenging assay by spectrophotometer. Result: Maximum inhibition of calcium oxalate crystallisation has been noticed in Sarsaparilla Mother Tincture and 1M potency. While Sarsaparilla 30C showing maximum inhibition of Calcium Phosphate crystallisation. Sarsaparilla mother tincture was able to decolorize during DPPH assay in which Inhibition % was decreased as potency increased (Q>30C>1M). Conclusion: The present in vitro study has shown potential role of Homoeopathic medicine Sarsaparilla as an inhibitor of calcium oxalate and calcium phosphate crystallisation.

In Silico and in Vitro Evaluation of Deamidation Effects on the Stability of the Fusion Toxin DAB389IL-2

2019 ◽  
Vol 16 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Nasrin Zarkar ◽  
Mohammad Ali Nasiri Khalili ◽  
Fathollah Ahmadpour ◽  
Sirus Khodadadi ◽  
Mehdi Zeinoddini
Keyword(s):  
In Silico ◽  
Catalytic Domain ◽  
Md Simulations ◽  
Special Importance ◽  
Native Form ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Pharmaceutical Protein ◽  
The Stability

Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and is the first immunotoxin approved by FDA. It is used for the treatment of various kinds of cancer such as CTCL lymphoma, melanoma, and Leukemia but among all of these, treatment of CTCL has special importance. DAB389IL-2 consists of two distinct parts; the catalytic domain of Diphtheria Toxin (DT) that genetically fused to the whole IL-2. Deamidation is the most important reaction for chemical instability of proteins occurs during manufacture and storage. Deamidation of asparagine residues occurs at a higher rate than glutamine residues. The structure of proteins, temperature and pH are the most important factors that influence the rate of deamidation. Methods: Since there is not any information about deamidation of DAB389IL-2, we studied in silico deamidation by Molecular Dynamic (MD) simulations using GROMACS software. The 3D model of fusion protein DAB389IL-2 was used as a template for deamidation. Then, the stability of deamidated and native form of the drug was calculated. Results: The results of MD simulations were showed that the deamidated form of DAB389IL-2 is more unstable than the normal form. Also, deamidation was carried by incubating DAB389IL-2, 0.3 mg/ml in ammonium hydrogen carbonate for 24 h at 37o C in order to in vitro experiment. Conclusion: The results of in vitro experiment were confirmed outcomes of in silico study. In silico and in vitro experiments were demonstrated that DAB389IL-2 is unstable in deamidated form.

Curcumin-based antioxidant and glycohydrolase inhibitor compounds: Synthesis and in vitro appraisal of the dual activity against diabetes

2020 ◽  
Vol 16 ◽  
Author(s):  
Sajjad Esmaeili ◽  
Nazanin Ghobadi ◽  
Donya Nazari ◽  
Alireza Pourhossein ◽  
Hassan Rasouli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibitor ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
Curcumin Derivatives ◽  
Reducing Power Assay

Background: Curcumin, as the substantial constituent of the turmeric plant (Curcuma longa), plays a significant role in the prevention of various diseases, including diabetes. It possesses ideal structure features as enzyme inhibitor, including a flexible backbone, hydrophobic nature, and several available hydrogen bond (H-bond) donors and acceptors. Objective: The present study aimed at synthesizing several novel curcumin derivatives and further evaluation of these compounds for possible antioxidant and anti-diabetic properties along with inhibitory effect against two carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase, as these enzymes are therapeutic targets for attenuation of postprandial hyperglycemia. Methods: Therefore, curcumin-based pyrido[2,3-d]pyrimidine derivatives were synthesized and identified using an instrumental technique like NMR spectroscopy and then screened for antioxidant and enzyme inhibitory potential. Total antioxidant activity, reducing power assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH• ) radical scavenging activity were done to appraisal the antioxidant potential of these compounds in vitro. Results: Compounds L6-L9 showed higher antioxidant activity while L4, L9, L12 and especially L8 exhibited the best selectivity index (lowest α-amylase/α-glucosidase inhibition ratio). Conclusion: These antioxidant inhibitors may be potential anti-diabetic drugs, not only to reduce glycemic index but also to limit the activity of the major reactive oxygen species (ROS) producing pathways.

Synthesis and antioxidant activity of new selenium-containing quinolines

2020 ◽  
Vol 16 ◽  
Author(s):  
Benedetta Bocchini ◽  
Bruna Goldani ◽  
Fernanda S.S. Sousa ◽  
Paloma T. Birmann ◽  
Cesar A. Brüning ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Radical Scavenging ◽  
Building Blocks ◽  
Antioxidant Potential ◽  
In Vitro Assays ◽  
Pharmacological Activities ◽  
Antioxidant Power ◽  
Antioxidant Agents

Background: Quinoline derivatives have been attracted much attention in drug discovery and synthetic derivatives of these scaffolds present a range of pharmacological activities. Therefore, organoselenium compounds are valuable scaffolds in organic synthesis because their pharmacological activities and their use as versatile building blocks for regio-, chemio-and stereoselective reactions. Thus, the synthesis of selenium-containing quinolines has great significance, and their applicability range from simple antioxidant agents, to selective DNA-binding and photocleaving agents. Objective: In the present study we describe the synthesis and antioxidant activity in vitro of new 7-chloroN(arylselanyl)quinolin-4-amines 5 by the reaction of 4,7-dichloroquinoline 4 with (arylselanyl)-amines 3. Methods: For the synthesis of 7-chloro-N(arylselanyl)quinolin-4-amines 5, we performed the reaction of (arylselanyl)- amines 3 with 4,7-dichloroquinoline 4 in the presence of Et3N at 120 °C in a sealed tube. The antioxidant activities of the compounds 5 were evaluated by the following in vitro assays: 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric ion reducing antioxidant power (FRAP), nitric oxide (NO) scavenging and superoxide dismutase-like activity (SOD-Like). Results: 7-Chloro-N(arylselanyl)quinolin-4-amines 5a-d has been synthesized in yields ranging from 68% to 82% by the reaction of 4,7-dichloroquinoline 4 with arylselanyl-amines 3a-d using Et3N as base, at 120 °C, in a sealed tube for 24 hours and tolerates different substituents, such as -OMe and -Cl, in the arylselanyl moiety. The obtained compounds 5a-d presented significant results with respect to the antioxidant potential, which had effect in the tests of inhibition of radical’s DPPH, ABTS+ and NO, as well as in the test that evaluates the capacity (FRAP) and in the superoxide dismutase-like activity assay (SOD-Like). It is worth mentioning that 7-chloro-N(arylselanyl)quinolin-4-amine 5b presented excellent results, demonstrating a better antioxidant capacity when compared to the others. Conclusion: According to the obtained results 7-chloro-N(arylselanyl)quinolin-4-amines 5 were synthesized in good yields by the reaction of 4,7-dichloroquinoline with arylselanyl-amines and tolerates different substituents in the arylselanyl moiety. The tested compounds presented significant antioxidant potential in the tests of inhibition of DPPH, ABTS+ and NO radicals, as well as in the FRAP and superoxide dismutase-like activity assays (SOD-Like).

scholarly journals Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Microwave Assisted Synthesis ◽  
Stable Free Radical ◽  
Ft Ir ◽  
Antimicrobial And Antioxidant Activity ◽  
New Compounds ◽  
Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

scholarly journals Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

Antioxidants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
Pattamaporn Aksornchu ◽  
Netima Chamnansilpa ◽  
Sirichai Adisakwattana ◽  
Thavaree Thilavech ◽  
Charoonsri Choosak ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Digestive Enzyme ◽  
Radical Scavenging ◽  
Protein Glycation ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Fruit Extract ◽  
Antioxidant Power ◽  
Ic50 Values

Antidesma bunius (L.) spreng (Mamao) is widely distributed in Northeastern Thailand. Antidesma bunius has been reported to contain anthocyanins, which possess antioxidant and antihypertensive actions. However, the antidiabetic and antiglycation activity of Antidesma bunius fruit extract has not yet been reported. In this study, we investigated the inhibitory activity of anthocyanin-enriched fraction of Antidesma bunius fruit extract (ABE) against pancreatic α-amylase, intestinal α-glucosidase (maltase and sucrase), protein glycation, as well as antioxidant activity. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) chromatogram revealed that ABE contained phytochemical compounds such as cyanidin-3-glucoside, delphinidin-3-glucoside, ellagic acid, and myricetin-3-galactoside. ABE inhibited intestinal maltase and sucrase activity with the IC50 values of 0.76 ± 0.02 mg/mL and 1.33 ± 0.03 mg/mL, respectively. Furthermore, ABE (0.25 mg/mL) reduced the formation of fluorescent AGEs and the level of Nε-carboxymethyllysine (Nε-CML) in fructose and glucose-induced protein glycation during four weeks of incubation. During the glycation process, the protein carbonyl and β-amyloid cross structure were decreased by ABE (0.25 mg/mL). In addition, ABE exhibited antioxidant activity through DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) with the IC50 values 15.84 ± 0.06 µg/mL and 166.1 ± 2.40 µg/mL, respectively. Meanwhile, ferric reducing antioxidant power (FRAP) showed an EC50 value of 182.22 ± 0.64 µg/mL. The findings suggest that ABE may be a promising agent for inhibiting carbohydrate digestive enzyme activity, reducing monosaccharide-induced protein glycation, and antioxidant activity.

scholarly journals Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 6
Author(s):  
Juan Esteban Oyarzún ◽  
Marcelo E. Andia ◽  
Sergio Uribe ◽  
Paula Núñez Pizarro ◽  
Gabriel Núñez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Cell Model ◽  
Radical Scavenging ◽  
Central Zone ◽  
Total Phenolic ◽  
Nonalcoholic Fatty Liver ◽  
Bee Pollen ◽  
Quercetin Concentration

Nonalcoholic fatty liver disease (NAFLD) is a major cause of morbidity and mortality worldwide. Additional therapies using functional foods and dietary supplements have been investigated and used in clinical practice, showing them to be beneficial. Honeybee pollen from Chile has shown a large concentration of phenolic compounds and high antioxidant activity. In this work, we characterized twenty-eight bee pollen extracts from the central zone of Chile according to botanical origin, phenolic profile, quercetin concentration, and antioxidant activity (FRAP and ORAC-FL). Our results show a statistically significant positive correlation between total phenolic content and antioxidant capacity. Selected samples were evaluated on the ability to reverse the steatosis in an in vitro cell model using Hepa1-6 cells. The pollen extracts protected Hepa1-6 cells against oxidative damage triggered by 2,2′-azo-bis(2-amidinopropane) dihydrochloride (AAPH)derived free radicals. This effect can be credited to the ability of the phenolic compounds present in the extract to protect the liver cells from chemical-induced injury, which might be correlated to their free radical scavenging potential. Additionally, bee pollen extracts reduce lipid accumulation in a cellular model of steatosis. In summary, our results support the antioxidant, hepatoprotective, and anti-steatosis effect of bee pollen in an in vitro model.

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