scholarly journals Monocytes as amajor population of peripheral blood cells expressing C-reactive protein

2020 ◽  
pp. 32-37
Author(s):  
О.А. Гусева ◽  
И.С. Мельников ◽  
Е.С. Зубкова ◽  
С.Г. Козлов ◽  
Ю.Н. Автаева ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Cell ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Blood Monocytes ◽  
C Reactive Protein ◽  
Peripheral Blood Monocytes ◽  
Reactive Protein

Данные крупных проспективных исследований демонстрируют корреляционную связь уровня С-реактивного белка (СРБ) в крови и риска развития неблагоприятных сердечно-сосудистых событий. Однако остаются малоизученными уровень экспрессии СРБ клетками пери- ферической крови и их способность синтезировать СРБ. Цель исследования. Определение уровней экспрессии СРБ циркулирующими клетками периферической крови. Исследование экспрессии мРНК СРБ макрофагами, полученными из моноцитов периферической крови. Материал и методы. Исследовали эритроциты, тромбоциты и лейкоциты периферической крови 6 добровольцев в возрасте от 30 до 60 лет. Для идентификации фенотипа клеток крови применяли метод проточной цитофлюориметрии с использованием панели моноклональных ан- тител, конъюгированных с различными флюорохромами, а именно CD235a-PE-Cy7, CD41-APC, CD45-PerCP-Cy5.5 и CD14-APC-Cy-7. Уровень экспрессии клетками крови СРБ определяли по уровню флюоресценции связанных с целевыми клетками FITC-конъюгированных моноклональ- ных антител к СРБ («ИМТЕК», Россия). Для определения мРНК СРБ применяли метод количественной полимеразной цепной реакции (ПЦР) с последующим анализом специфичности амплификаций с помощью электрофореза в агарозном геле. Об экспрессии СРБ макрофагами, полученными из моноцитов периферической крови, судили по количеству мРНК, выделенной после их активации липополисахаридом (ЛПС). Результаты. Результаты исследования показали, что СРБ экспрессируют 85,0±10,5% моноцитов; лимфоциты, тромбоциты и эритроци- ты — 7,5±0,6, 3,0±0,3 и 4,3±0,5% соответственно. Методом количественной ПЦР в небольших количествах мРНК СРБ была зарегистри- рована в макрофагах, активированных ЛПС. Ее уровень незначительно, в 0,79±0,73 раза (p=0,96, n=6), отличался относительно гена «домашнего хозяйства». Заключение. Обнаружено, что СРБ присутствует на внешней клеточной мембране до 90% циркулирующих моноцитов и до 10% лимфо- цитов, в то время как эритроциты и тромбоциты не несут на своей поверхности СРБ. Установлена возможность синтеза СРБ стимулиро- ванными ЛПС макрофагами, полученными из моноцитов периферической крови. Data from major prospective studies demonstrate a correlation between blood C-reactive protein (CRP) levels and the risk of adverse cardiovascular events. However, the level of expression of CRP by peripheral blood cells and their ability to synthesize CRP remains poorly studied. The purpose of the study. Determination of CRP expression levels by circulating peripheral blood cells. Investigation of expression of CRP mRNA by macrophages obtained from peripheral blood monocytes. Material and methods. erythrocytes, platelets and leukocytes of peripheral blood were studied in 6 volunteers aged 30 to 60 years. To identify the blood cell phenotype, the method of flow cytofluorimetry was used using a panel of monoclonal antibodies conjugated with different fluorophores, namely, CD235a-PE-Cy7, CD41-APC, CD45-PerCP-Cy5.5 and CD14-APC-Cy-7. Blood cell expression level of CRP was determined by fluorescence level of FITC-conjugated monoclonal antibodies to CRP associated with target cells (IMTEK, Russia). The method of quantitative polymerase chain reaction (PCR) with the subsequent analysis of specificity of amplifications by electrophoresis in agarose gel was used for determination of mRNA of CRP. The expression of DRR by macrophages derived from peripheral blood monocytes was judged by the amount of mRNA isolated after their activation by lipopolysaccharide (LPS). The results. The results of the study showed that CRP express 85.0±10.5% monocytes, lymphocytes, platelets and red blood cells — 7.5±0.6, 3.0±0.3 and 4.3±0.5% respectively. The method of quantitative PCR in small amounts of CRP mRNA was registered in macrophages activated by LPS. Its level was insignificant, by 0.79±0.73 times (p=0.96, n=6), differed from the "household" gene. Conclusion. It was found that CRP is present on the outer cell membrane up to 90% of circulating monocytes and up to 10% of lymphocytes, while red blood cells and platelets do not carry CRP on their surface. The possibility of synthesis of CRP by stimulated LPS macrophages obtained from peripheral blood monocytes has been established

Sites of Tissue Factor Pathway Inhibitor (TFPI) and Tissue Factor Expression under Physiologic and Pathologic Conditions

1995 ◽  
Vol 73 (05) ◽  
pp. 873-875 ◽  
Author(s):  
Bjarne Østerud ◽  
Madhu S Bajaj ◽  
Paul S Bajaj
Keyword(s):  
Tissue Factor ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Tissue Factor Pathway Inhibitor ◽  
Peripheral Blood Cells ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Inflammatory Conditions ◽  
Factor Expression ◽  
Tissue Factor Pathway

SummaryUnder normal conditions, TFPI expression is restricted to megakaryocytes and the endothelium of the microvasculature. It is not synthesized by normal hepatocytes or by the endothelium of larger vessels. In contrast, endothelium and peripheral blood cells do not express tissue factor under normal conditions. Expression of tissue factor under normal physiologic conditions is widespread and is localized in areas which are physically separated from the circulating blood. During an inflammatory response, circulating monocytes have been shown to express tissue factor, whereas in general, expression of tissue factor has not been observed in the endothelium. Adherent monocytes/macrophages express both tissue factor and TFPI under pathologic conditions. Whether or not circulating peripheral blood monocytes under inflammatory conditions express TFPI is not known.

The Effect of C-Reactive Protein (CRP) on Respiratory Burst of Human Peripheral Blood Phagocytes

1995 ◽  
Vol 8 (2) ◽  
pp. 95-102
Author(s):  
J. Cofta ◽  
J.K. Lacki ◽  
S.H. Mackiewicz ◽  
K.E. Wiktorowicz
Keyword(s):  
Respiratory Burst ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Low Doses ◽  
Blood Monocytes ◽  
C Reactive Protein ◽  
Free Oxygen ◽  
Peripheral Blood Monocytes ◽  
Reactive Protein ◽  
Enhanced Chemiluminescence

The effect of C-reactive protein (CRP) on the oxidative response of human peripheral blood monocytes and granulocytes was investigated. The respiratory burst of phagocytes induced by phorbol-myristate-13-acetate (PMA), phytohaemagglutinin (PHA) or opsonized zymosan (OZ) was measured in luminol-enhanced chemiluminescence. The effect of CRP on PMA-induced monocyte chemiluminescence (CL) depended both on CRP concentration and incubation time. A short incubation of cells with CRP (15–30 min.) enhanced the oxidative burst. Preincubation of cells for 1h (or longer) with low doses of CRP (about 2 μg/ml) increased, while with higher (>10 μg/ml) inhibited PMA-stimulated chemiluminescence. CRP reduced also PHA or OZ-induced monocyte respiratory response. CRP diminished PMA, PHA, and OZ-induced granulocyte chemiluminescence, except the response to PHA in the presence of low doses CRP (about 5 μg/ml). The action of CRP on phagocytes probably involves activation of some intracellular mechanisms. During immune response, CRP could protect tissues against damage by excess of free oxygen and its biological active derivatives.

Determination of PD-1 expression in peripheral blood cells in patients with endometriosis

2020 ◽  
pp. 1-5
Author(s):  
Buğra Okşaşoğlu ◽  
Ceylan Hepokur ◽  
Sema Misir ◽  
Çağlar Yildiz ◽  
Gamze Sönmez ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells

scholarly journals Chromosome Studies in Normal and Leukemic Rats

Blood ◽  
1964 ◽  
Vol 23 (5) ◽  
pp. 564-571 ◽  
Author(s):  
G. DOWD ◽  
K. DUNN ◽  
WILLIAM C. MOLONEY
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Species Differences ◽  
Peripheral Blood Cell ◽  
Myelogenous Leukemia ◽  
Peripheral Blood Cells ◽  
X Ray ◽  
Normal Rat

Abstract 1. Adequate chromosome preparations were obtained in 70 per cent of normal rat peripheral blood cell cultures. However, cultures of peripheral blood cells from leukemic rats were almost universally unsuccessful. 2. In x-ray- and 3MCA-induced leukemias direct bone marrow preparations provided adequate metaphases in eight of 12 cases. Failures were attributed in four cases to scanty material obtained from fibrotic marrows. 3. No consistent chromosome abnormalities, such as those reported in human myelogenous leukemia, were found in these leukemic rats. However, the series of cases is small, and species differences and other factors may have influenced the results of these studies.

scholarly journals Concanavalin A-mediated binding and sphering of human red blood cells by homologous monocytes.

1976 ◽  
Vol 144 (6) ◽  
pp. 1695-1700 ◽  
Author(s):  
D Guerry ◽  
M A Kenna ◽  
A D Schrieber ◽  
R A Cooper
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Concanavalin A ◽  
Blood Cells ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
Con A ◽  
Peripheral Blood Monocytes ◽  
Human Red Blood Cells ◽  
Human Rbcs

Human red blood cells sensitized with concanavalin A became bound to homologous peripheral blood monocytes. Binding occured at a concentration of 10(5) molecules of tetrameric Con A per red blood cell (RBC) and increased with additional Con A. RBC binding began within 5 min and was maximal at 90 min. Phagocytosis of sensitized RBCs was minimal. RBC attachment was prevented by 0.01 M alpha-methyl-D-mannopyranoside, and, once the RBC-monocyte rosette was established, bound RBCs were largely removed with this specific saccharide inhibitor of Con A. RBCs attached to monocytes became spherocytic and osmotically fragile. The recognition of concanavalin A (Con A)-coated RBCs was not mediated through the monocyte IgG-Fc receptor. These studies demonstrate that, like IgG and C3b, Con A is capable of mediating the binding of human RBCs to human monocytes. Red cells so bound are damaged at the monocyte surface.

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