Long‐term effects of omalizumab on peripheral blood cells and C‐reactive protein levels in patients with chronic spontaneous urticaria

2019 ◽  
pp. e12966 ◽  
Author(s):  
Neslihan Akdogan ◽  
Neslihan Demirel Ogut ◽  
Sibel Dogan ◽  
Nilgun Atakan
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Chronic Spontaneous Urticaria ◽  
Peripheral Blood Cells ◽  
Long Term Effects ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein

scholarly journals Monocytes as amajor population of peripheral blood cells expressing C-reactive protein

2020 ◽  
pp. 32-37
Author(s):  
О.А. Гусева ◽  
И.С. Мельников ◽  
Е.С. Зубкова ◽  
С.Г. Козлов ◽  
Ю.Н. Автаева ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Cell ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Blood Monocytes ◽  
C Reactive Protein ◽  
Peripheral Blood Monocytes ◽  
Reactive Protein

Данные крупных проспективных исследований демонстрируют корреляционную связь уровня С-реактивного белка (СРБ) в крови и риска развития неблагоприятных сердечно-сосудистых событий. Однако остаются малоизученными уровень экспрессии СРБ клетками пери- ферической крови и их способность синтезировать СРБ. Цель исследования. Определение уровней экспрессии СРБ циркулирующими клетками периферической крови. Исследование экспрессии мРНК СРБ макрофагами, полученными из моноцитов периферической крови. Материал и методы. Исследовали эритроциты, тромбоциты и лейкоциты периферической крови 6 добровольцев в возрасте от 30 до 60 лет. Для идентификации фенотипа клеток крови применяли метод проточной цитофлюориметрии с использованием панели моноклональных ан- тител, конъюгированных с различными флюорохромами, а именно CD235a-PE-Cy7, CD41-APC, CD45-PerCP-Cy5.5 и CD14-APC-Cy-7. Уровень экспрессии клетками крови СРБ определяли по уровню флюоресценции связанных с целевыми клетками FITC-конъюгированных моноклональ- ных антител к СРБ («ИМТЕК», Россия). Для определения мРНК СРБ применяли метод количественной полимеразной цепной реакции (ПЦР) с последующим анализом специфичности амплификаций с помощью электрофореза в агарозном геле. Об экспрессии СРБ макрофагами, полученными из моноцитов периферической крови, судили по количеству мРНК, выделенной после их активации липополисахаридом (ЛПС). Результаты. Результаты исследования показали, что СРБ экспрессируют 85,0±10,5% моноцитов; лимфоциты, тромбоциты и эритроци- ты — 7,5±0,6, 3,0±0,3 и 4,3±0,5% соответственно. Методом количественной ПЦР в небольших количествах мРНК СРБ была зарегистри- рована в макрофагах, активированных ЛПС. Ее уровень незначительно, в 0,79±0,73 раза (p=0,96, n=6), отличался относительно гена «домашнего хозяйства». Заключение. Обнаружено, что СРБ присутствует на внешней клеточной мембране до 90% циркулирующих моноцитов и до 10% лимфо- цитов, в то время как эритроциты и тромбоциты не несут на своей поверхности СРБ. Установлена возможность синтеза СРБ стимулиро- ванными ЛПС макрофагами, полученными из моноцитов периферической крови. Data from major prospective studies demonstrate a correlation between blood C-reactive protein (CRP) levels and the risk of adverse cardiovascular events. However, the level of expression of CRP by peripheral blood cells and their ability to synthesize CRP remains poorly studied. The purpose of the study. Determination of CRP expression levels by circulating peripheral blood cells. Investigation of expression of CRP mRNA by macrophages obtained from peripheral blood monocytes. Material and methods. erythrocytes, platelets and leukocytes of peripheral blood were studied in 6 volunteers aged 30 to 60 years. To identify the blood cell phenotype, the method of flow cytofluorimetry was used using a panel of monoclonal antibodies conjugated with different fluorophores, namely, CD235a-PE-Cy7, CD41-APC, CD45-PerCP-Cy5.5 and CD14-APC-Cy-7. Blood cell expression level of CRP was determined by fluorescence level of FITC-conjugated monoclonal antibodies to CRP associated with target cells (IMTEK, Russia). The method of quantitative polymerase chain reaction (PCR) with the subsequent analysis of specificity of amplifications by electrophoresis in agarose gel was used for determination of mRNA of CRP. The expression of DRR by macrophages derived from peripheral blood monocytes was judged by the amount of mRNA isolated after their activation by lipopolysaccharide (LPS). The results. The results of the study showed that CRP express 85.0±10.5% monocytes, lymphocytes, platelets and red blood cells — 7.5±0.6, 3.0±0.3 and 4.3±0.5% respectively. The method of quantitative PCR in small amounts of CRP mRNA was registered in macrophages activated by LPS. Its level was insignificant, by 0.79±0.73 times (p=0.96, n=6), differed from the "household" gene. Conclusion. It was found that CRP is present on the outer cell membrane up to 90% of circulating monocytes and up to 10% of lymphocytes, while red blood cells and platelets do not carry CRP on their surface. The possibility of synthesis of CRP by stimulated LPS macrophages obtained from peripheral blood monocytes has been established

scholarly journals Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jong-Hyeok Jung ◽  
Hyun Jung Hong ◽  
Aziz Gharderpour ◽  
Jae Young Cho ◽  
Bum-Seo Baek ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Renal Scarring ◽  
Long Term Effects ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Serum Crp

Abstract Urinary tract infection (UTI) is one of the most common bacterial infections in infants less than age 1 year. UTIs frequently recur and result in long-term effects include sepsis and renal scarring. Uropathogenic Escherichia coli (UPEC), the most prevalent organism found in UTIs, can cause host inflammation via various virulence factors including hemolysin and cytotoxic necrotizing factors by inducing inflammatory cytokines such as interleukin (IL)-1β. However, the ability of each UPEC organism to induce IL-1β production may differ by strain. Furthermore, the correlation between differential IL-1β induction and its relevance in pathology has not been well studied. In this study, we isolated UPEC from children under age 24 months and infected bone-marrow derived macrophages with the isolates to investigate secretion of IL-1β. We found that children with higher concentrations of C-reactive protein (CRP) were more likely to harbor phylotype B2 UPEC strains that induced more IL-1β production than phylotype D. We also observed a significant correlation between serum CRP level and in vitro IL-1β induction by phylotype B2 UPEC bacteria. Our results highlight the diversity of UPEC in terms of IL-1β induction capacity in macrophages and suggest a potential pathogenic role in UTIs by inducing inflammation in infants.

scholarly journals Long-Term Effects of Pravastatin on Plasma Concentration of C-reactive Protein

Circulation ◽  
1999 ◽  
Vol 100 (3) ◽  
pp. 230-235 ◽  
Author(s):  
Paul M. Ridker ◽  
Nader Rifai ◽  
Marc A. Pfeffer ◽  
Frank Sacks ◽  
Eugene Braunwald
Keyword(s):  
Plasma Concentration ◽  
Long Term Effects ◽  
C Reactive Protein ◽  
Reactive Protein

Phenotypic Analysis of Spleen, Thymus, and Peripheral Blood Cells in Aged C57BI/6 Mice Following Long-Term Exposure to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin

1995 ◽  
Vol 25 (1) ◽  
pp. 60-69
Author(s):  
JULIE A. OUGHTON ◽  
CLIFFORD B. PEREIRA ◽  
GREG K. DEKREY ◽  
JOHN M. COLLIER ◽  
ANTHONY A. FRANK ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Phenotypic Analysis ◽  
Tetrachlorodibenzo P Dioxin

Long-Term Stability of High Sensitivity C Reactive Protein Levels in Healthy Adults

2007 ◽  
Vol 16 ◽  
pp. S68
Author(s):  
B.M. McQuillan ◽  
E. Rossi ◽  
J. Hung ◽  
P.L. Thompson ◽  
J.P. Beilby
Keyword(s):  
High Sensitivity ◽  
Healthy Adults ◽  
C Reactive Protein ◽  
Term Stability ◽  
Protein Levels ◽  
Long Term Stability ◽  
Reactive Protein

Association between percutaneous coronary intervention and long-term C-reactive protein levels in patients with acute coronary syndromes

2010 ◽  
Vol 30 (1) ◽  
pp. 10-13 ◽  
Author(s):  
Kausik K. Ray ◽  
Babak Nazer ◽  
Richard Cairns ◽  
C. Michael Gibson ◽  
Christopher P. Cannon
Keyword(s):  
Percutaneous Coronary Intervention ◽  
Acute Coronary Syndromes ◽  
Coronary Intervention ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Percutaneous Coronary ◽  
Coronary Syndromes
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