Polymorphism of Ukrainian common winter wheat cultivars with respect to the molecular marker for the gene conferring moderate resistance against Fusarium head blight

Goal. The goal of the work was to evaluate the genetic potential of resistance to Fusarium head blight of collections of winter common wheat cultivars based on the allelic state of the TDF_076_2D gene conferring moderate resistance against Fusarium graminearum Schwabe and F. culmorum (W.G.Sm.) Sacc. fungi. Materials and methods. We studied 68 winter common wheat cultivars developed in different scientific-breeding institutions of Ukraine (the Myronivka Remeslo Institute of Wheat of NAAS, the Plant Breeding and Genetics Institute (PBGI), the Рlant Production Institute nd. a. V. Ya. Yuryev of NAAS, Poltava State Agrarian Academy (PSAA)). A silica-based commercial kit was used for DNA extraction. To detect the allelic state of the resistance gene, the INDEL1 marker cosegregating with the TDF_076_2D gene was used. Amplified fragments obtained as a result of PCR were separated in 3% agarose gel and visualized with use of ethidium bromide. Results. The frequency of the resistance allele according to the INDEL1 marker for the gene conferring moderate resistance to the Fusarium fungi ranged from 0.682 in the sample of PBGI cultivars to 0.882 in the PSAA group; the frequency of the resistance allele in the total sample made up 0.782. It was found that the cultivar ‘Poliovyk’ showed a non-typical pattern of amplified bands, which possibly indicates the allele for susceptibility to Fusarium head blight. Conclusions. The majority of the common wheat cultivars from the studied sample carry the resistance allele of the marker for the gene of interest. The data obtained are consistent with the results of the previous research of the wider sample of the winter and spring common wheat cultivars. The cultivars with the resistance allele might show a lower infection level in the field and serve as a source of the gene in marker assisted selection. The cultivar ‘Poliovyk’ is worth more detailed studying with use of other molecular markers for the TDF_076_2D gene and (or) sequencing to find out a precise allelic state of the gene.

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Genetic background for moderate resistance against fusarium head blight among winter wheat developed in the Forrest Steppe of Ukraine

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v26.1249 ◽

2020 ◽

Vol 26 ◽

pp. 96-100

Author(s):

A. V. Karelov ◽

N. A. Kozub ◽

I. I. Kucheriavy ◽

O. I. Sozinova ◽

I. O. Sozinov ◽

...

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Common Wheat ◽

Genetic Background ◽

Resistance Allele ◽

Wheat Cultivars ◽

Head Blight ◽

Infection Level ◽

Allelic State ◽

Moderate Resistance

Aim. The aim of the work was to evaluate the genetic background of resistance to Fusarium head blight in common winter wheat cultivars based on the allelic state of the TDF_076_2D gene conferring tolerance against Fusarium graminearum Schwabe and F. culmorum (W.G.Sm.) Sacc. fungi. Methods. We studied 91 winter common wheat cultivars developed in the Institute of Plant Physiology and Genetics of NAS of Ukraine. A silica-based commercial kit was used for DNA extraction. For the allelic state detection, the INDEL1 marker co-segregating with the TDF_076_2D gene was used. Results. The frequency of the resistance allele according to the marker for the gene conferring moderate resistance to the Fusarium fungi made up 0.802. Conclusions. The majority of the common wheat cultivars from the studied sample carry the resistance allele of the gene of interest. The data obtained are consistent with the results of previous research for the wider sample of the winter and spring common wheat cultivars. The cultivars with confirmed resistance allele might show lower infection level in the field and serve as a source of the gene in marker assisted selection. Keywords: common wheat, disease resistance genes, Fusarium head blight, molecular markers.

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Evaluation of resistance to Fusarium head blight in wheat using different sources of inoculum

Czech Journal of Genetics and Plant Breeding ◽

10.17221/112/2011-cjgpb ◽

2011 ◽

Vol 47 (No. 4) ◽

pp. 131-139 ◽

Cited By ~ 4

Author(s):

V. Šíp ◽

J. Chrpová ◽

L. Štočková

Keyword(s):

Fusarium Head Blight ◽

Yield Components ◽

Yield Loss ◽

Wheat Cultivars ◽

Head Blight ◽

Spray Inoculation ◽

Symptom Scores ◽

Winter Wheat Cultivars ◽

Moderate Resistance ◽

Different Sources

The response of four winter wheat cultivars, differing in resistance to Fusarium head blight (FHB), to spray inoculation with four selected Fusarium graminearum isolates, mixture of these isolates and frequently used F. culmorum isolate B was studied in five field and glasshouse experiments during 2008–2010. Analyses of variance showed highly significant main and interaction effects of cultivar, inoculum source and environment (year-trial) on all five examined traits indicative of disease severity, yield loss and accumulation of mycotoxins. The relations between traits were not evidently influenced by the used isolate. Resistance of host genotypes and environmental conditions accounted for a greater proportion of the total variation (8–36%) than the inoculation source (isolate) that substantially influenced the accumulation of the mycotoxin deoxynivalenol (12%), but expressed relatively low effects on symptom scores, percentage of fusarium damaged kernels and reductions of yield components (2–4%). Two-way and three-way interactions accounted for 25–40% of variation in the examined traits, which indicates great importance of multi-environment tests, using different Fusarium isolates for inoculation. Separate inoculation with F. graminearum isolates, differing in aggressiveness, did not appear to be more advantageous than their use in mixture that showed medium or below-average aggressiveness in all traits. The application of an isolate mixture could be recommended as a “less costly” alternative to inoculation with single isolates in trials repeated in different years and/or locations. It was indicated by these experiments that especially the detection of resistance/moderate resistance to FHB could be facilitated by the use of a carefully selected mixture of isolates. However, the application of aggressive isolates (isolate B of F. culmorum in these experiments) appeared to be beneficial to eliminate FHB susceptible materials in the breeding process.  

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Improvement of Resistance of Wheat Cultivars to Fusarium Head Blight in the Yellow-Huai Rivers Valley Winter Wheat Zone with Functional Marker Selection of Fhb1 Gene

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2018.00505 ◽

2018 ◽

Vol 44 (4) ◽

pp. 505 ◽

Cited By ~ 5

Author(s):

Hong-Jun ZHANG ◽

Zhen-Qi SU ◽

Gui-Hua BAI ◽

Xu ZHANG ◽

Hong-Xiang MA ◽

...

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Functional Marker ◽

Wheat Cultivars ◽

Head Blight ◽

Marker Selection ◽

Selection Of

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Fusarium Head Blight and Moniliformin Accumulation in Kernels of 18 Winter Wheat Cultivars Inoculated with Fusarium Avenaceum (3 Years Study)

Cereal Research Communications ◽

10.1007/bf03543810 ◽

1997 ◽

Vol 25 (3) ◽

pp. 673-675 ◽

Cited By ~ 6

Author(s):

Piotr Goliński ◽

Marian Kostecki ◽

Przemysław Kaptur ◽

Slawomir Wojciechowski ◽

Zygmunt Kaczmarek ◽

...

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Fusarium Avenaceum ◽

Wheat Cultivars ◽

Head Blight ◽

Winter Wheat Cultivars

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Reaction to Fusarium Head Blight (FHB) of Six New Wheat Cultivars Widely Grown in Transylvania

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:3980 ◽

1970 ◽

Vol 66 (1) ◽

Author(s):

Sardar AMIN ◽

Marin ARDELEAN ◽

Vasile MOLDOVAN ◽

Rodica CADAR

Keyword(s):

Grain Yield ◽

Fusarium Head Blight ◽

Wheat Cultivars ◽

Head Blight ◽

Fusarium Spp ◽

Developmental Progress ◽

Progress Curve ◽

Symptom Intensity ◽

Winter Wheat Cultivars ◽

Artificial Inoculations

Fusarium head blight (FHB), caused by Fusarium spp., has become one of the most destructive diseases in the world’s wheat growing areas , especially in the humid and semihumid regions (Paillard et al. 2004, Mesterhazy 1978, Stack & Mullen 1985; Kiecana 1987; Kiecana et al. 1988). Six winter wheat cultivars, recently released and widely grown in Trasylvania, have been evaluated for FHB resistance during 2006. The evaluation was made by means of artificial inoculations with Fusarium graminearum and assesment of symptom intensity by computing AUDPC index (Area Under Developmental Progress Curve). Based on these data, two cultivars (Dumbrava and Turda 195) were considered as resistant, two cultivars (Ardeal and Arieşan) as medium resistant and other two cultivars (Fundulea 4 and GK Öthalom) as susceptible to FHB. The reaction to FHB of the six tested cultivars, based on the postharvest indices, showed significant differences among these cultivars as far as the yield elements and the total grain yield were considered. Actually, in the resistant cultivars, both the total grain yield and some of the yield elements (spike wight, grain weght/spike and 1000 kernel weigt) were significantly less affected by FHB than in medium resistant and susceptible cultivars.

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