scholarly journals PRODUCTION OF LIPASE BY IMMOBILIZED BACILLUS THURINGIENSIS AND LYSINIBACILLUS SPHAERICUS AND THEIR BIODEGRADATION POTENTIAL ON DIESEL

2020 ◽  
Vol 3 (3) ◽  
pp. 52-57
Author(s):  
Olaposi Adegoke ◽  
Sarafadeen Kareem ◽  
Saka Balogun ◽  
Adeniyi Afolabi
Keyword(s):  
Bacillus Thuringiensis ◽  
16S Rrna ◽  
Mineral Salt Medium ◽  
Lipase Production ◽  
Mineral Salt ◽  
Oil Degradation ◽  
Salt Medium ◽  
Gelatin Matrix ◽  
Lysinibacillus Sphaericus ◽  
Ms Analysis

This study reported production of lipase by immobilized Bacillus thuringiensis. Bacteria isolates were screened on Bushnell-Hass Mineral Salt medium containing 1% v/v Diesel for oil degradation. The potent isolates were identified using 16S rRNA as Bacillus thuringiensis. The isolates were immobilized in gelatin matrix and cultured for lipase production in a submerged medium. The crude lipase extracted was used for degradation of Diesel. Optimum degradation of Diesel 41.4% was obtained by lipase from Immobilized Bacillus thuringiensis and 31.6% for Lysinibacillus sphaericus at pH 7 and 35 in 20 days. GC-MS analysis was carried out to show the compounds degraded after 20 days. This study therefore presented the use of immobilized bacterial lipase in degradation of Diesel as a simple and effective approach.

Metabolism of a monoterpene alcohol, linalool, by a soil pseudomonad

1977 ◽  
Vol 23 (3) ◽  
pp. 230-239 ◽  
Author(s):  
K. M. Madyastha ◽  
P. K. Bhattacharyya ◽  
C. S. Vaidyanathan
Keyword(s):  
Mineral Salt Medium ◽  
Enrichment Culture ◽  
Sole Source ◽  
Mineral Salt ◽  
Salt Medium ◽  
Culture Techniques ◽  
Unsaturated Lactone ◽  
Linalool Oxide

A microorganism of the genus Pseudomonas has been isolated from the soil by enrichment culture techniques with linalool(I) as the sole source of carbon and energy. The organism is also capable of utilizing limonene, citronellol, and geraniol as substrates but fails to grow on citral, citranellal, and 1,8-cineole. Fermentation of linalool by this bacterium in a mineral salt medium results in the formation of 10-hydroxylinalool(II), 10-carboxylinalool(III), oleuropeic acid(IX), 2-vinyl-2-methyl-5-hydroxyisopropyl-tetrahydrofuran(linalool oxide, V), 2-vinyl-2-methyl-tetrahydrofuran-5-one(unsaturated lactone, VI), and few unidentified minor metabolites. Probable pathways for the biodegradation of linalool are presented.

scholarly journals Removal of tannin from Shea nut cake by Pseudomonas strain

1970 ◽  
Vol 5 (1) ◽  
pp. 17-22
Author(s):  
F.M. Danikuu ◽  
A. Quainoo ◽  
E.N.K. Sowley
Keyword(s):  
Mineral Salt Medium ◽  
Cost Effective ◽  
Sole Source ◽  
Northern Region ◽  
Mineral Salt ◽  
Salt Medium ◽  
Biomedical Sciences ◽  
Nutritional Factors ◽  
Tannin Concentration ◽  
Enhanced Biodegradation

The use of microorganisms to remove the anti-nutritional factors in shea nut cake as the most cost effective and environmentally friendly approach to permit its use in agriculture for animal nutrition and soil fertility is long anticipated. Bacteria isolated from shea nut cake polluted soil in Sagnarigu District of Northern Region of Ghana showed that anti-nutritional factors in shea nut cake are bio-degradable. The bacteria were grown in mineral salt medium supplemented with 2% shea nut cake as sole source of carbon. The bacteria isolate was identified biochemically as Pseudomonas aerugenosa and reduced total tannin concentration in shea nut cake from 54.58 g Kg-1 to 8.71 g Kg-1 (84%) in 10 days and 92% in 20 days. Boiling of shea nut cake reduced tannin content from 54.58 g Kg-1 to 16.36 g Kg-1 (70%) and enhanced biodegradation of tannin in the shea nut cake, removing up to 95% of total tannins in shea nut cake in 20 days.Journal of Medical and Biomedical Sciences (2016) 5(1), 17-22Keywords: Tannins, biodegradability, bacteria, mineral salt medium

scholarly journals Enrichment and Analysis of Stable 1,4-dioxane-Degrading Microbial Consortia Consisting of Novel Dioxane-Degraders

2019 ◽  
Vol 8 (1) ◽  
pp. 50 ◽  
Author(s):  
Tanmoy Roy Tusher ◽  
Takuya Shimizu ◽  
Chihiro Inoue ◽  
Mei-Fang Chien
Keyword(s):  
Ribosomal Rna ◽  
Industrial Wastewater ◽  
Microbial Consortium ◽  
Mineral Salt Medium ◽  
Intergenic Spacer ◽  
Mineral Salt ◽  
Microbial Consortia ◽  
Salt Medium ◽  
Bacterial Strains ◽  
Water Contaminant

Biodegradation of 1,4-dioxane, a water contaminant of emerging concern, has drawn substantial attention over the last two decades. A number of dioxane-degraders have been identified, though many of them are unable to metabolically utilize 1,4-dioxane. Moreover, it is considered more preferable to use microbial consortia rather than the pure strains, especially in conventional bioreactors for industrial wastewater treatment. In the present study, a stable 1,4-dioxane-degrading microbial consortium was enriched, namely 112, from industrial wastewater by nitrate mineral salt medium (NMSM). The consortium 112 is capable of utilizing 1,4-dioxane as a sole carbon and energy source, and can completely degrade 1,4-dioxane up to 100 mg/L. From the consortium 112, two 1,4-dioxane-degrading bacterial strains were isolated and identified, in which the Variovorax sp. TS13 was found to be a novel 1,4-dioxane-degrader that can utilize 100 mg/L of 1,4-dioxane. The efficacy of the consortium 112 was increased significantly when we cultured the consortium with mineral salt medium (MSM). The new consortium, N112, could utilize 1,4-dioxane at a rate of 1.67 mg/L·h. The results of the ribosomal RNA intergenic spacer analysis (RISA) depicted that changes in the microbial community structure of consortium 112 was the reason behind the improved degradation efficiency of consortium N112, which was exhibited as a stable and effective microbial consortium with a high potential for bioremediation of the dioxane-impacted sites and contaminated industrial wastewater.

Thermokinetic responses of the metabolic activity of Staphylococcus lentus cultivated in a glucose limited mineral salt medium

2010 ◽  
Vol 104 (1) ◽  
pp. 149-155 ◽  
Author(s):  
Sudharshan Sekar ◽  
Surianarayanan Mahadevan ◽  
Sathish Sundar Dhilip Kumar ◽  
Asit Baran Mandal
Keyword(s):  
Metabolic Activity ◽  
Mineral Salt Medium ◽  
Mineral Salt ◽  
Salt Medium

scholarly journals ISOLASI DAN KARAKTERISTIK BAKTERI LIGNOLITIK DARI LIMBAH PEWARNA TENUN IKAT

2018 ◽  
Vol 11 (01) ◽  
pp. 11-17
Author(s):  
Yulita I. Mamulak
Keyword(s):  
Mineral Salt Medium ◽  
Enrichment Culture ◽  
Mineral Salt ◽  
Salt Medium

Limbah pewarna yang dihasilkan oleh industry tenun ikat berpotensi mencemari lingkungan dan mengandung berbagai jenis mikrobia yang berkemampuan untuk mendegradasi pewarna. Penelitian ini bertujuan untuk memperoleh isolat bakteri yang mampu mendegradasi limbah pewarna industri tenun ikat. Isolasi bakteri dilakukan dengan teknik enrichment culture (pengkayaan) pada media MSM (Mineral Salt Medium). Hasil isolasi dari 4 sampel limbah diperoleh 35 isolat bakteri, seleksi kualitatif menghasilkan 12 isolat unggul yang berkemampuan lignolitik dengan daya lignolitik berkisar antara 7,75 – 19,75. Dari seleksi kuantitatif berdasarkan kemampuan dekolorisasi pewarna dihasilkan 3 isolat unggul yaitu isolat bakteri IN1, IN7 dan C5. Identifikasi awal menunjukkan bahwa ketiga isolat memiliki kedekatan dengan genus Enterobacter dan Brucella.   Kata kunci:  Bakteri, dekolorisasi dan limbah pewarna

Sign in / Sign up

Export Citation Format

Share Document