scholarly journals Biological and Biochemical factors Predictive of Oocyte survival, Fertilization, Pregnancy in oocyte thawing cycles

2021 ◽  
pp. 1-12
Author(s):  
Enrica Capitanio ◽  
Alessia Galimberti ◽  
Laura Zanga ◽  
Federica Paternostro ◽  
Sara Melis ◽  
...  
Keyword(s):  
Fertilization Rate ◽  
Clinical Pregnancy ◽  
Clinical Factors ◽  
Ongoing Pregnancy ◽  
Ongoing Pregnancy Rate ◽  
Vitro Fertilization ◽  
Factors Affecting ◽  
Thawing Cycle ◽  
Oocyte Freezing

Optimization and monitoring of IVF treatments requires good data on the effect and magnitude of clinical factors affecting treatment outcome. Many factors have been known to affect IVF outcomes. Currently there are still no data to predict whether a patient who undergoes In Vitro Fertilization (IVF) cycles can be considered a good candidate for oocyte freezing. The aim of this study was therefore to evaluate which biological and biochemical factors can be predictive of oocyte survival and fertilization, as well as of clinical pregnancy in oocyte thawing cycles. This study showed that none of the factors available on the day of the pick-up is able to predict (in case of oocyte cryopreservation) the success of a subsequent oocyte thawing cycle. Only the transfer of at least one Grade 1 embryo after oocyte thawing cycle has a statistically significant impact on pregnancy. Unfortunately, this cannot be considered an elective factor to guide the clinician and/or the embryologist in choosing patient's treatment as it is not available on the day of the oocyte pick up but it is a result of oocyte thawing. Keywords: Oocyte thawing; Biological and biochemical markers; Fertilization rate; Ongoing pregnancy rate

Development and implementation of intracytoplasmic sperm injection (ICSI)

1995 ◽  
Vol 7 (2) ◽  
pp. 211 ◽  
Author(s):  
GD Palermo ◽  
J Cohen ◽  
M Alikani ◽  
A Adler ◽  
Z Rosenwaks
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Human Sperm ◽  
Fertilization Rate ◽  
Semen Parameters ◽  
Ongoing Pregnancy Rate ◽  
Vitro Fertilization ◽  
Human Oocytes ◽  
Intracytoplasmic Injection ◽  
Previously Treated

The purpose of this paper is to elucidate the experimental steps that led to the development of intracytoplasmic sperm injection (ICSI) and its application in the human. ICSI has become the most successful micromanipulation procedure for treating male infertility. A total of 355 in vitro fertilization (IVF) cycles utilizing ICSI are described; 180 couples were previously treated in 509 IVF cycles but achieved no fertilization and 175 couples could not be treated by IVF because of extremely poor semen parameters. Of the 3063 metaphase II (M II) oocytes retrieved, 2970 were injected with a survival rate of 93.6%, yielding 1917 bipronuclear zygotes (64.5%). In 148 patients, a foetal heart was evidenced by ultrasound; 11 of these patients miscarried between 7 and 13 weeks of gestation. The ongoing pregnancy rate was 38.6% (137/355) per retrieval and 40.5% (137/338) per embryo replacement. At the time of writing, there were 22 deliveries and one therapeutic abortion for a trisomy 21 chromosomal abnormality. In addition, 66 singleton, 37 twin, 10 triplet and 1 quadruplet pregnancies were ongoing. The concentration of motile spermatozoa in the ejaculate only slightly influenced the fertilization rate (P < 0.001) and the pregnancy outcome (P < 0.01). A preliminary injection procedure utilizing intracytoplasmic injection of isolated sperm heads was performed in 35 M II human oocytes with resultant fertilization and cleavage rates of 74% and 73% respectively. Skills in ICSI were acquired by injecting hamster and unfertilized human oocytes with human sperm. ICSI can be used to successfully treat couples who have failed IVF or who have too few spermatozoa for conventional in vitro insemination.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Progesterone levels predict pregnancy outcome in individuals with fallopian tube associated infertility

2020 ◽  
Author(s):  
Wenjia BO ◽  
Ning Zhang
Keyword(s):  
Fallopian Tube ◽  
Clinical Data ◽  
Pregnancy Outcomes ◽  
Birth Rate ◽  
Fertilization Rate ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Group 3 ◽  
Group 2

Abstract Background: To investigate the predictive value of human chorionic gonadotropin(HCG) and progesterone(P) levels on pregnancy outcomes in patients receiving in vitro fertilization(IVF) due to simple fallopian tube factors. METHODS:We retrospectively analyzed the clinical data of 854 cycles from the simple fallopian tube factor IVF fresh embryo transfer.The clinical data of 854 cycles from January 2010 to December 2018 was divided into 7 groups according to the P level on HCG day.Live birth rates(LBR) and observe trends were calculated. The receiver operating characteristic(ROC) curve was established to determine the optimal cutoff value for P, which was used to further divide the data into 3 groups: Group 1 (P ≦ 1.0 ng/ml), Group 2 (1.0 ng/ml ≤ P ≤ 1.25 ng/ml), and Group 3 (P≥1.25ng/ml). We then compared the ovulation results and clinical outcomes between the 3 groups. RESULTS There were no significant differences in age, infertility years, Gonadotropin(Gn)dosage, Gn days, Luteinizing hormone(LH) level on HCG day, 2pronuclear(2PN) fertilization rate, clinical pregnancy rate(CPR), LBR, full-term birth rate, and preterm birth rate among the three groups, but body mass index （BMI）(P = 0.001), basal LH (P = 0.034), estrogen peak (P = 0.000), number of eggs obtained (P = 0.000) were significantly different. CONCLUSION The level of P level on HCG day does not affect the CPR and LBR after IVF. However, P levels between 1.0-1.25ng/ml may lead to good clinical pregnancy outcomes.

scholarly journals Association between Vitamin D Levels and Fertility Outcomes in Patients Undergoing IVF/ICSI

2020 ◽  
Vol 02 (03) ◽  
pp. 85-92
Author(s):  
Anh N. Ha ◽  
Toan D. Pham ◽  
Lan N. Vuong
Keyword(s):  
Vitamin D ◽  
Pregnancy Rates ◽  
Ongoing Pregnancy ◽  
Ongoing Pregnancy Rate ◽  
Metabolizing Enzymes ◽  
Vitro Fertilization ◽  
Increased Risk ◽  
Vitamin D Levels ◽  
Icsi Outcomes

Background: Several studies have demonstrated that vitamin D (vitD) might play an important role in the reproductive system due to expression of vitD receptor and vitD-metabolizing enzymes in many reproductive tissues. VitD deficiency has been associated with increased risk of obstetric complications. However, the effect of vitD levels on in vitro fertilization (IVF)/ICSI outcomes is not fully understood. Evidence shows that women with adequate vitD levels might have higher pregnancy rates. This study evaluated the association between serum vitD levels and IVF/ICSI outcomes. Methods: This multicenter, retrospective cohort study was conducted at IVFMD, My Duc Hospital and IVFMDPN, My Duc Phu Nhuan Hospital, Ho Chi Minh City, Vietnam between November 2017 and July 2019. Vietnamese patients aged 18–40 years with serum vitD (25(OH)D) samples collected before starting controlled ovarian stimulation and undergoing embryo transfer were eligible. Patients were divided into four groups based on 25(OH)D levels: <10 ng/mL, 10 to <20 ng/mL, 20 to <30 ng/mL, and [Formula: see text]30 ng/mL. The primary outcome was ongoing pregnancy rate. Results: Of 3779 patients recruited, 25(OH)D levels were <10 ng/mL in 564 (14.9%), 10 to <20 ng/mL in 436 (11.5%), 20 to <30 in 1,142 (30.2%), and [Formula: see text]30 ng/mL in 1,637 (43.3%). Ongoing pregnancy rates were similar across the four subgroups (36%, 40%, 36%, and 36%, respectively; p = 0.409). The number of oocytes retrieved, embryos, clinical pregnancy, implantation, and miscarriage rates did not differ significantly between subgroups. Conclusions: In this analysis, serum vitD levels did not appear to be correlated with pregnancy outcomes in patients undergoing IVF/ICSI.

scholarly journals Does Endometriosis Influence the Embryo Quality and/or Development? Insights from a Large Retrospective Matched Cohort Study

Diagnostics ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 83 ◽  
Author(s):  
Ana M. Sanchez ◽  
Luca Pagliardini ◽  
Greta C. Cermisoni ◽  
Laura Privitera ◽  
Sofia Makieva ◽  
...  
Keyword(s):  
Potential Effect ◽  
Fertilization Rate ◽  
Women Patients ◽  
Cleavage Stage ◽  
Ongoing Pregnancy Rate ◽  
Vitro Fertilization ◽  
Morphological Evaluation ◽  
Negative Effect

In vitro fertilization can be an effective tool to manage the endometriosis-associated infertility, which accounts for 10% of the strategy indications. Nevertheless, a negative effect of endometriosis on IVF outcomes has been suggested. The aim of this study was to evaluate the potential effect of endometriosis in the development of embryos at cleavege stage in assisted reproduction treatment cycles. A total of 429 cycles from women previously operated for moderate/severe endometriosis were compared with 851 cycles from non-affected women. Patients were matched by age, number of oocyte retrieved and study period. A total of 3818 embryos in cleavage stage have been analyzed retrospectively. Overall, no difference was found between women with and without endometriosis regarding the number of cleavage stage embryos obtained as well as the percentage of good/fair quality embryos. Excluding cycles in which no transfers were performed or where embryos were frozen in day three, no difference was observed for blastulation rate or the percentage of good/fair blastocysts obtained. Despite similar fertilization rate and number/quality of embryos, a reduction in ongoing pregnancy rate was observed in patients affected, possibly due to an altered endometrial receptivity or to the limited value of the conventional morphological evaluation of the embryo.

scholarly journals Relationship Between Anti-Müllerian Hormone and In Vitro Fertilization-Embryo Transfer in Clinical Pregnancy

2020 ◽  
Vol 11 ◽  
Author(s):  
Xing Yu Sun ◽  
Yun Zhu Lan ◽  
Shuang Liu ◽  
Xiao Ping Long ◽  
Xi Guang Mao ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Endometrial Thickness ◽  
Antral Follicle ◽  
Clinical Pregnancy ◽  
Multivariate Logistic Regression Model ◽  
Vitro Fertilization ◽  
Factors Affecting ◽  
Infertile Women ◽  
Ivf Et

ObjectivesTo retrospectively analyze the correlation between anti-Müllerian hormone (AMH) and the number of oocytes obtained by controlled ovarian hyperstimulation (COH) in women of different ages and explore the factors affecting in vitro fertilization and embryo transfer (IVF-ET) in clinical pregnancy of infertile women to provide evidence for infertile women to choose assisted reproduction strategies.MethodsInfertile women who received IVF-ET or intracytoplasmic sperm injection and embryo transfer (ICSI-ET) treatment in the reproductive center of XX hospital between October 2018 and September 2019 were included. Patient data on medical records, age, body mass index (BMI), years of infertility, basic follicle-stimulating hormone (FSH), basic luteinizing hormone (LH), basic estradiol (E2), anti-Müllerian hormone level (AMH), antral follicle count (AFC), gonadotropins (Gn) medication days, Gn dosage, endometrial thickness on transplantation day, the number of retrieved oocytes, the number of mature oocytes obtained, the number of embryos transferred, clinical pregnancy status, etc., were collected.ResultsA total of 314 patients were enrolled in this study, with an average age of 31.0 ± 4.5 years. The infertility period ranged from 0–21 years. The AMH level showed a downward trend with increasing age. Overall, the AMH level of women of all ages was positively correlated with the number of retrieved oocytes (r = 0.335, p &lt; 0.001). The AMH level of women between 22 and 28 years old was positively correlated with the number of retrieved oocytes (r = 0.164, p &lt; 0.061) but it was not statistically significant. Similarly, the AMH level of women aged 29–35 and 36–43 was positively correlated with the number of retrieved oocytes (r = 0.356, p &lt; 0.001; r = 0.461, p &lt; 0.001). The average age of the pregnant group (30.6 ± 4.4 years) was lower than that of the non-pregnant group (32.2 ± 4.6 years) (p &lt; 0.001). The number of oocytes obtained (9.8 ± 4.5) and the number of embryos transferred (1.9 ± 0.4) in the pregnant group was significantly higher than that in the non-pregnant group (9.2 ± 4.5; 1.7 ± 0.5); the difference was statistically significant. The multivariate logistic regression model showed that age (OR = 0.574 95% CI: 0.350–0.940), AMH (OR = 1.430 95% CI: 1.130–1.820) and the number of oocytes obtained (OR = 1.360 95% CI: 1.030–1.790) were factors affecting clinical pregnancy.ConclusionWe found that the level of AMH in infertile women decreased with age and the number of oocytes obtained in infertile women was positively correlated with AMH. Moreover, the number of oocytes and embryo transferred in the pregnant group was significantly higher than those in the non-pregnant group. Furthermore, age, AMH and the number of oocytes affected the clinical pregnancy.

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