Cadmium-induced root growth inhibition is mediated by hydrogen peroxide production in root tip of Arabidopsis

2010 ◽  
Vol 18 (1) ◽  
pp. 136-140 ◽  
Author(s):  
Si-Nan ZHANG ◽  
Pei-Yao GAO ◽  
Qing-En XIE ◽  
Xu-Hua ZHAO ◽  
Xia LI
PROTOPLASMA ◽  
2004 ◽  
Vol 224 (1-2) ◽  
pp. 91-98 ◽  
Author(s):  
M. šimonovičová ◽  
J. Huttová ◽  
I. Mistrík ◽  
B. široká ◽  
L. Tamás

2013 ◽  
Vol 59 (No. 2) ◽  
pp. 62-67 ◽  
Author(s):  
V. Zelinová ◽  
B. Bočová ◽  
J. Huttová ◽  
I. Mistrík ◽  
L. Tamás

We analyse the effect of Cd and H<sub>2</sub>O<sub>2</sub> short-term treatments on the activity of ascorbate-glutathione recycling enzymes in barley root tip. Even a short transient exposure of barley roots to low 15 &micro;mol Cd concentration caused a marked approximately 70% root growth inhibition. Higher Cd concentrations caused root growth cessation during the first 6 h after short-term Cd treatment. Similarly, a marked root growth inhibition was also detected after the short-term exposure of barley seedlings to H<sub>2</sub>O<sub>2</sub>. Our results indicate that root ascorbate pool is more sensitive to Cd treatment than glutathione pool. Rapid activation of dehydroascorbate reductase and monodehydroascorbate reductase is the important component of stress response to the Cd-induced alterations in barley root tips. H<sub>2</sub>O<sub>2</sub> is probably involved in the Cd-induced activation of monodehydroascorbate reductase, but it is not involved in the Cd-induced increase of dehydroascorbate reductase activity.


Author(s):  
Doris Fovwe Ogeleka ◽  
Esther Obasi

Introduction: The constant impact on the environment occasioned by pollution, indiscriminate application of agricultural chemicals, security challenges and crisis in the Niger Delta ecological area of Nigeria has caused severe damage to plants, soil organisms and humans. Aim and Methodology: In this research, onions (Allium cepa L) was exposed to varying concentrations of an atrazine-based selective herbicide Arda-force® to estimate the phyto-toxic effects on the plant species using the Organization for Economic Co-operation and Development, (OECD) protocol #208. Results: The mean effective concentration (EC50) using root growth inhibition produced indications of phyto-toxicity to the exposed species at a concentration of 0.55 ± 0.06 mg/L. Similarly, the maximum root growth inhibition efficiency relative to the control was 65% as recorded in the highest test concentration of 1.25 mg/L. Discussion: The study indicated that constant application / indiscriminate use of the herbicide Arda-force® could cause deleterious influence on these plant and vegetable species, daily consumed by humans as a rich source of anti-oxidants. Conclusion: This study concluded that atrazine-based herbicide Arda-force® used in this assessment resulted in phyto-toxic effects to Allium cepa L. At the exposed concentrations of the herbicide to non-target specie – Allium cepa L. that are integral parts of the ecosystems, the ‘‘harmless’’ status of atrazine acclaimed by the United State Environmental Protection Agency (USEPA) is still very much in doubt.


2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Meiting Du ◽  
Yanhong Wang ◽  
Huize Chen ◽  
Rong Han

Abstract Background UV-B signaling in plants is mediated by UVR8, which interacts with transcriptional factors to induce root morphogenesis. However, research on the downstream molecules of UVR8 signaling in roots is still scarce. As a wide range of functional cytoskeletons, how actin filaments respond to UV-B-induced root morphogenesis has not been reported. The aim of this study was to investigate the effect of actin filaments on root morphogenesis under UV-B and hydrogen peroxide exposure in Arabidopsis. Results A Lifeact-Venus fusion protein was used to stain actin filaments in Arabidopsis. The results showed that UV-B inhibited hypocotyl and root elongation and caused an increase in H2O2 content only in the root but not in the hypocotyl. Additionally, the actin filaments in hypocotyls diffused under UV-B exposure but were gathered in a bundle under the control conditions in either Lifeact-Venus or uvr8 plants. Exogenous H2O2 inhibited root elongation in a dose-dependent manner. The actin filaments changed their distribution from filamentous to punctate in the root tips and mature regions at a lower concentration of H2O2 but aggregated into thick bundles with an abnormal orientation at H2O2 concentrations up to 2 mM. In the root elongation zone, the actin filament arrangement changed from lateral to longitudinal after exposure to H2O2. Actin filaments in the root tip and elongation zone were depolymerized into puncta under UV-B exposure, which showed the same tendency as the low-concentration treatments. The actin filaments were hardly filamentous in the maturation zone. The dynamics of actin filaments in the uvr8 group under UV-B exposure were close to those of the control group. Conclusions The results indicate that UV-B inhibited Arabidopsis hypocotyl elongation by reorganizing actin filaments from bundles to a loose arrangement, which was not related to H2O2. UV-B disrupted the dynamics of actin filaments by changing the H2O2 level in Arabidopsis roots. All these results provide an experimental basis for investigating the interaction of UV-B signaling with the cytoskeleton.


Plant Science ◽  
2020 ◽  
Vol 293 ◽  
pp. 110418
Author(s):  
Iñigo Saiz-Fernández ◽  
Maite Lacuesta ◽  
Usue Pérez-López ◽  
M. Carmen Sampedro ◽  
Ramon J. Barrio ◽  
...  

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