cDNA Library Construction and Expressed Sequence Tags(ESTs) Analyses of an Alpine Plant Species Gentiana officinalis(Gentianaceae)

2009 ◽  
Vol 31 (2) ◽  
pp. 146-152
Author(s):  
Hong-Ju YIN ◽  
Liu-Yang WANG ◽  
Qian GAO ◽  
Da-Hai GAO ◽  
Dong-Yuan ZHANG ◽  
...  
Keyword(s):  
Cdna Library ◽  
Plant Species ◽  
Expressed Sequence Tags ◽  
Cdna Library Construction ◽  
Alpine Plant ◽  
Library Construction ◽  
Expressed Sequence

scholarly journals Construction of a cDNA Library of Vitis pseudoreticulata Native to China Inoculated with Uncinula necator and the Analysis of Potential Defense-related Expressed Sequence Tags

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 993C-993
Author(s):  
Yan Xu ◽  
Yuejin Wang
Keyword(s):  
Cdna Library ◽  
Plant Species ◽  
Expressed Sequence Tags ◽  
Cdna Clones ◽  
Sequence Information ◽  
Uncinula Necator ◽  
The Public ◽  
Perennial Plant ◽  
Vitis Pseudoreticulata ◽  
Expressed Sequence

Expressed sequence tags (ESTs) constitute a rapid and informative strategy for studying gene-expression profiles of specific stages of annual and perennial plant species. Compared with annual plants, the NCBI database has very little sequence information from perennial plant species. To date, only ∼145 ESTs of Vitis pseudoreticulata W.T. Wang have been deposited in databases. This is insufficient to understand the biology and development of this species. In this report, a cDNA library constructed from young leaf inoculated with powdery mildew pathogen [Uncinula necator (Schw.) Burr.] of Chinese wild Vitis pseudoreticulata. Leaf was harvested at various times after inoculation for total RNA extraction, which was used to generate ESTs. In our study, 107 cDNA clones were sequenced either from 5' or 3' end of the cDNAs. Among them, 60 unigenes (56%) were functionally characterized by the BLASTX matches to known function proteins, and 20 unigenes (18.6 %) matched significantly with those having unknown function in the public databases. The remaining 27 unigenes (25.2%) failed to show significant homology to any proteins in the public databases, suggesting that they represent novel sequences. Some functional genes identified from the cDNA library to be potentially associated with plant defence-related responses are discussed.

cDNA library construction of two human Demodexspecies

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
DongLing Niu ◽  
RuiLing Wang ◽  
YaE Zhao ◽  
Rui Yang ◽  
Li Hu ◽  
...  
Keyword(s):  
Cdna Library ◽  
Cdna Library Construction ◽  
Library Construction

AbstractThe research of

scholarly journals Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

2014 ◽  
Vol 13 (2) ◽  
pp. 378-386
Author(s):  
Su-mei ZHAO ◽  
Yong-gang LIU ◽  
Hong-bing PAN ◽  
Xi ZHANG ◽  
Chang-rong GE ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Full Length ◽  
Full Length Cdna ◽  
Expressed Sequence

Analysis of 2,297 expressed sequence tags (ESTs) from a cDNA library of flax (Linum ustitatissimum L.) bark tissue

2012 ◽  
Vol 39 (5) ◽  
pp. 6289-6296 ◽  
Author(s):  
Song-Hua Long ◽  
Xin Deng ◽  
Yu-Fu Wang ◽  
Xiang Li ◽  
Rui-Qing Qiao ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Bark Tissue ◽  
Expressed Sequence
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