Application of Different Rates and Frequencies of Rejuvenator for the Treatments of Brown Bast Syndrome on Hevea brasiliensis

Background: Brown bast is one of the most serious threats to natural rubber production. It is estimated that brown bast leads to an approximately 15-20% decrease in latex yield production. There is currently no effective method to control this problem and an effort to overcome this problem is in progress by the natural rubber producing countries. Methods: This study evaluated the effect and the most suitable rate and frequency of Rejuvenator application to cure brown bast (latex dryness) and improve latex yield. The Rejuvenator treatment was applied to the selected tree with two different treatment frequencies (every 3 days and every 6 days) and three different Rejuvenator concentrations (5 g/L, 10 g/L and 5 g/L). The Rejuvenator was applied 5 times. Data on latex yield and brown bast cure were then recorded 1 month after the application of the last treatment. The treatments used were T1: No Rejuvenator, T2: 5 g/L of Rejuvenator, T3: 10 g/L of Rejuvenator, T4: 15 g/L of Rejuvenator. Result: Of all the treatment used, T3 (10 g/L) resulted in higher production of latex yield and Hevea bark nutrient concentration (g/t/t) than control. It also gave the highest nutrient content in the bark tissue. The findings of this study reveal that the optimum concentration and frequency of Rejuvenator application were established at 10 g/L every 6 days. More so, the findings explain that inadequate nutrients can be attributed to the brown bast syndrome in rubber plantation which can be prevented with the use of a rejuvenator to the tree’s bark.

Peculiarities of selecting woody plants for anatomy analysis in various environments

Methods for studying woody plants anatomy are now very diverse. We modified the guidelines for bark studies developed for wood analysis at all stages of preparing the woody plant samples for microscope study. For the first time, we used separation to separate hard and soft fractions of the bark tissue. Correct approach to selection of plant samples and further laboratory manipulations ensures validity of the results of our study. We select the size of the sampling area depending on the type of vegetation. In every habitat, we also include transects along the gradient of height above sea level or the impact from the source of natural stress. The results of our research will help study landscape changes during exogenous geological processes and phenomena using biological indication of geosystems.

Wood and bark water content and monthly stem growth in Amazonian tree species

ABSTRACT The knowledge of how trees respond to microclimate variability is important in the face of climate changes. The objectives of this study were to examine the variation in wood water content (WWC) and bark water content (BWC) in Amazonian trees, as well as to assess the effect of microclimatic variability on monthly diameter growth rates (DGR). We extracted a core sample from each of 120 trees (28 species) and determined WWC and BWC on a fresh matter basis. DGR was measured monthly during the 12 months of 2007. The effect of microclimatic variability on DGR was analyzed by redundancy analysis. Average BWC and WWC were 53.4% and 34.7%, respectively, with a large variation in stem water content among species (BWC = 36.2−67.1%; WWC = 26.4−50.8%). There was no significant relationship between stem diameter and WWC or BWC, nor between DGR and wood density (p > 0.05). However, wood density was negatively correlated with WWC (r s = −0.69, p < 0.001). The high BWC emphasizes the importance of the bark tissue in Amazonian trees. Contrary to expectations, variability of monthly irradiance, rainfall and temperature had no effect on DGR (p > 0.20). The unresponsiveness of DGR to microclimatic variability, even in an above-average rainy year such as 2007, indicates that other parts of the tree may have greater priority than the stem for carbon allocation during the dry season.

Status of Charcoal Canker on Oak Trees at a Site of Community Importance: Case Study of the Relict Castelfidardo Forest (SIC Area IT520008, Castelfidardo, AN, Italy)

Oaks are dominant and key tree species in Mediterranean forest ecosystems. However, in recent decades, oak forests have been heavily impacted by oak decline, a worldwide phenomenon exacerbated by climate change. The charcoal disease agent Biscogniauxia mediterranea is involved in the decline of Mediterranean oak formations in a variety of contexts. Here, we investigated the impact and role of B. mediterranea in the decline of oaks in Castelfidardo Forest, a relict wood of the late Holocene and a Site of Community Importance. We established five plots within which we recorded tree positions, any symptoms and signs of decline, association of B. mediterranea to declining trees, and deadwood and associated mycota. Of 471 oaks inspected, 7.0% showed brownish exudates on the stems, 46.9% showed epicormic shoots along the main trunk, and 24.4% showed black carbonaceous stromata on diseased branches and trunks. The decline was most severe for Quercus cerris, which comprised plots #4 and #5, at 50.0% (81/162 trees) and 29.0% (33/114), respectively; then for Quercus robur for plot #3, at 40.0% (38/95); and finally for Quercus pubescens for plots #1 and #2, at 13.7% (7/51) and 12.3% (6/49), respectively. Bark tissue was collected from trees with charcoal cankers via microscopy examination and identified by mycological and molecular methods. This investigation revealed a close association between oaks with pronounced reduction of vitality and incidence of B. mediterranea. Deadwood was equally distributed among the five plots, and was heavily colonized by Basidiomycota. The high incidence of the charcoal canker pathogen B. mediterranea appeared to be related to environmental stresses. However, the absence of silvicultural management, high competition among physiologically mature trees, and the geographic isolation of this residual forest may have predisposed oaks to decline.

Nickel in forests – a short review on its distribution and fluxes

The distribution and cycling of nickel (Ni) in forests is greatly affected by their proximity to emission sources of the metal. The throughfall deposition is always richer in Ni than the bulk deposition. It can be inferred that some dry deposition enriches the throughfall. In remote forested areas, the hydrological fluxes of Ni do not differ a lot from those in litterfall. In addition, the current year needles in conifers have higher concentrations than the older needles, a sign of absorption and mobility of the metal. In contrast, near an industrial Ni source the older needles accumulate much more of the metal. The Ni content in bark tissue can be used to map the deposition distribution of the metal around an area (rural or urban). The concentrations of Ni in forest soils is also dependent on their distances from the Ni emission sources and the nature of the soil parent material. The Ni concentrations increase with soil depth due to the geogenic origin of the metal. Low pH greatly enhances the mobility of the metal in soils, much more than the leachability of organic matter.

Impact of Charcoal Canker on Oak Trees at a Site of Community Importance: Case Study of the Relict Castelfidardo Forest (SIC Area IT520008, Castelfidardo, AN, Italy)

Oaks are a dominant and key tree species in Mediterranean forest ecosystems. However, in recent decades, oak forests have been heavily impacted by oak decline, a worldwide phenomenon exacerbated by climate change. The charcoal disease agent Biscogniauxia mediterranea is involved in the decline of Mediterranean oak formations in a variety of contexts. Here, we investigated the impact and role of B. mediterranea in decline of oaks in Castelfidardo Forest, a relict wood of the late Holocene and a Site of Community Importance. We established five plots within which we recorded the tree positions, any symptoms and signs of decline, association of B. mediterranea to declining trees, and the deadwood and associated mycota. Of 471 oaks inspected, 7.0% showed brownish exudates on the stems, 46.9% showed epicormic shoots along the main trunk, and 24.4% showed black carbonaceous stromata on diseased branches and trunks. The decline was most severe for Quercus cerris, which comprised plots #4 and #5, at 50.0% (81/162 trees) and 29.0% (33/114), respectively; then for Quercus robur for plot #3, at 40.0% (38/95); and finally for Quercus pubescens for plots #1 and #2, at 13.7% (7/51) and 12.3% (6/49), respectively. Bark tissue was collected from trees with charcoal cankers, with microscopy examination and identification by mycological and molecular methods. This investigation revealed close association between oaks with pronounced reduction of vitality and incidence of B. mediterranea. The deadwood was equally distributed among the five plots, and was heavily colonized by Basidiomycota. The high incidence of this oak-weakening pathogen B. mediterranea appears to be related to stress conditions (e.g., water scarcity, prolonged drought). However, absence of silvicultural management, high competition among physiologically mature trees, and the geographic isolation of this residual forest might have predisposed these oaks to decline.

Roles of ethylene, jasmonic acid, and salicylic acid and their interactions in frankincense resin production in Boswellia sacra Flueck. trees

The roles of ethylene, jasmonic acid, and salicylic acid and their interactions in frankincense resin production in Boswellia sacra trees growing in the drylands of Oman were studied. On March 18 (Experiment 1) and September 17 (Experiment 2), 2018, 32-year-old B. sacra trees with multiple trunks were selected at the Agricultural Experiment Station, Sultan Qaboos University, Oman. Various lanolin pastes containing Ethrel, an ethylene-releasing compound; methyl jasmonate; sodium salicylate; and combinations of these compounds were applied to debarked wounds 15 mm in diameter on the trunks. After a certain period, the frankincense resin secreted from each wound was harvested and weighed. The anatomical characteristics of the resin ducts were also studied in the bark tissue near the upper end of each wound. The combination of Ethrel and methyl jasmonate greatly enhanced frankincense resin production within 7 days in both seasons. The application of methyl jasmonate alone, sodium salicylate alone or a combination of both did not affect resin production. These findings suggest a high possibility of artificial enhancement of frankincense resin production by the combined application of Ethrel and methyl jasmonate to B. sacra trees.

Bark tissue transcriptome analyses of inverted Populus yunnanensis cuttings reveal the crucial role of plant hormones in response to inversion

Inverted cuttings of Populus yunnanensis exhibit an interesting growth response to inversion. This response is characterized by enlargement of the stem above the shoot site, while the upright stem shows obvious outward growth below the shoot site. In this study, we examined transcriptome changes in bark tissue at four positions on upright and inverted cuttings of P. yunnanensis: position B, the upper portion of the stem; position C, the lower portion of the stem; position D, the bottom of new growth; and position E, the top of new growth. The results revealed major transcriptomic changes in the stem, especially at position B, but little alteration was observed in the bark tissue of the new shoot. The differentially expressed genes (DEGs) were mainly assigned to four pathways: plant hormone signal transduction, plant-pathogen interaction, mitogen-activated protein kinase (MAPK) signaling pathway-plant, and adenosine triphosphate-binding cassette (ABC) transporters. Most of these DEGs were involved in at least two pathways. The levels of many hormones, such as auxin (IAA), cytokinin (CTK), gibberellins (GAs), ethylene (ET), and brassinosteroids (BRs), underwent large changes in the inverted cuttings. A coexpression network showed that the top 20 hub unigenes at position B in the upright and inverted cutting groups were associated mainly with the BR and ET signaling pathways, respectively. Furthermore, brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) in the BR pathway and both ethylene response (ETR) and constitutive triple response 1 (CTR1) in the ET pathway were important hubs that interfaced with multiple pathways.