scholarly journals Construction of a cDNA Library of Vitis pseudoreticulata Native to China Inoculated with Uncinula necator and the Analysis of Potential Defense-related Expressed Sequence Tags

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 993C-993
Author(s):  
Yan Xu ◽  
Yuejin Wang
Keyword(s):  
Cdna Library ◽  
Plant Species ◽  
Expressed Sequence Tags ◽  
Cdna Clones ◽  
Sequence Information ◽  
Uncinula Necator ◽  
The Public ◽  
Perennial Plant ◽  
Vitis Pseudoreticulata ◽  
Expressed Sequence

Expressed sequence tags (ESTs) constitute a rapid and informative strategy for studying gene-expression profiles of specific stages of annual and perennial plant species. Compared with annual plants, the NCBI database has very little sequence information from perennial plant species. To date, only ∼145 ESTs of Vitis pseudoreticulata W.T. Wang have been deposited in databases. This is insufficient to understand the biology and development of this species. In this report, a cDNA library constructed from young leaf inoculated with powdery mildew pathogen [Uncinula necator (Schw.) Burr.] of Chinese wild Vitis pseudoreticulata. Leaf was harvested at various times after inoculation for total RNA extraction, which was used to generate ESTs. In our study, 107 cDNA clones were sequenced either from 5' or 3' end of the cDNAs. Among them, 60 unigenes (56%) were functionally characterized by the BLASTX matches to known function proteins, and 20 unigenes (18.6 %) matched significantly with those having unknown function in the public databases. The remaining 27 unigenes (25.2%) failed to show significant homology to any proteins in the public databases, suggesting that they represent novel sequences. Some functional genes identified from the cDNA library to be potentially associated with plant defence-related responses are discussed.

scholarly journals Expressed Sequence Tags (ESTs) from a Jute (Corchorus olitorius) cDNA Library

1970 ◽  
Vol 16 (2) ◽  
pp. 95-104 ◽  
Author(s):  
J Matthew Taliaferro ◽  
Ahmad S Islam ◽  
Kanagasabapathi Sathasivan
Keyword(s):  
Arabidopsis Thaliana ◽  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Higher Plants ◽  
Open Reading Frame ◽  
Sequence Information ◽  
Corchorus Olitorius ◽  
Reading Frame ◽  
Partial Cdna ◽  
Expressed Sequence

Analysis of the genome of jute (Corchorus olitorius) was done by creating a new cDNA library of expressed sequence tags (ESTs) in pBluescript as the previous libraries reported earlier in this journal yielded only small DNA fragments from chloroplast and mitochondrial DNA. This report discusses results from a cDNA library constructed using poly A+ mRNA purified from 7-day old etiolated jute seedlings. Out of 700 recombinant plasmids obtained, 250 were analyzed using WU-BLAST (www.arabidopsis.org) for similar EST sequences in Arabidopsis thaliana and other higher plants. So far the analysis of the library has yielded several significant sequences, including the complete open reading frame of the 60S acidic ribosomal protein P3 and a partial cDNA of Class I chitinase. These results and future EST sequences from this library will be made available in Genbank and the sequence information will be used to clone full length DNA through PCR.  DOI = 10.3329/ptcb.v16i2.1110Plant Tissue Cult. & Biotech. 16(2): 95-104, 2006 (December)

Analysis of expressed sequence tags of the water flea Daphnia magna

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 606-609 ◽  
Author(s):  
Hajime Watanabe ◽  
Norihisa Tatarazako ◽  
Shigeto Oda ◽  
Hiroyo Nishide ◽  
Ikuo Uchiyama ◽  
...  
Keyword(s):  
Daphnia Magna ◽  
Expressed Sequence Tags ◽  
Atp Synthesis ◽  
Amino Acid Sequences ◽  
Cdna Clones ◽  
Sequence Information ◽  
Water Flea ◽  
Expression Of Genes ◽  
Study Gene Expression ◽  
Expressed Sequence

To study gene expression in the water flea Daphnia magna we constructed a cDNA library and characterized the expressed sequence tags (ESTs) of 7210 clones. The EST sequences clustered into 2958 nonredundant groups. BLAST analyses of both protein and DNA databases showed that 1218 (41%) of the unique sequences shared significant similarities to known nucleotide or amino acid sequences, whereas the remaining 1740 (59%) showed no significant similarities to other genes. Clustering analysis revealed particularly high expression of genes related to ATP synthesis, structural proteins, and proteases. The cDNA clones and EST sequence information should be useful for future functional analysis of daphnid biology and investigation of the links between ecology and genomics.Key words: Daphnia magna, EST, classification, ATP synthesis.

Sequencing of cDNA clones and analysis of the expressed sequence tags (ESTs) of tea plant [Camellia sinensis (L.) O. Kuntze] young shoots

2005 ◽  
Vol 2 (2) ◽  
pp. 137-141
Author(s):  
Zhao Li-Ping ◽  
Gao Qi-Kang ◽  
Chen Liang
Keyword(s):  
Cdna Library ◽  
Camellia Sinensis ◽  
Expressed Sequence Tags ◽  
Tea Plant ◽  
Cdna Libraries ◽  
Functional Genes ◽  
Cdna Clones ◽  
Est Sequencing ◽  
Plant Ests ◽  
Expressed Sequence

AbstractThe construction of two cDNA libraries from young shoots of tea plant [Camellia sinensis cv. Longjing 43 and Anji Baicha], the sequencing of Longjing 43 cDNA clones and the analysis of expressed sequence tags (ESTs) are reported. Totally, 4320 clones from the cDNA library of Longjing 43 were sequenced, and 2963 useful sequences were obtained, corresponding to 68.6% of clones. A total of 416 clones shorter than 150 bp and 863 repeated clones were excluded, the first 1684 valid tea plant ESTs were generated. Most of the ESTs were between 300 and 700 bp, with an average of 478 bp. Six hundred and seven ESTs with known function or putative function were identified by BlastN searches against the National Center of Biotechnology Institute (NCBI) non-redundant nucleotide databases, corresponding to more than 130 functional genes in the tea plant. The rest, 1077 ESTs, were novel gene partial or full sequences. The results indicated that EST sequencing was a rapid and effective approach to identification of novel functional genes for tea plant.

scholarly journals Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

2014 ◽  
Vol 13 (2) ◽  
pp. 378-386
Author(s):  
Su-mei ZHAO ◽  
Yong-gang LIU ◽  
Hong-bing PAN ◽  
Xi ZHANG ◽  
Chang-rong GE ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Full Length ◽  
Full Length Cdna ◽  
Expressed Sequence
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