Embryonic Stem Cells Form Articular Cartilage, not Teratomas, in Osteochondral Defects of Rat Joints

2004 ◽  
Vol 13 (4) ◽  
pp. 331-336 ◽  
Author(s):  
Shigeyuki Wakitani ◽  
Hideyuki Aoki ◽  
Yasuji Harada ◽  
Masato Sonobe ◽  
Yusuke Morita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Osteochondral Defects

scholarly journals A scaffold-free approach to cartilage tissue generation using human embryonic stem cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lauren A. Griffith ◽  
Katherine M. Arnold ◽  
Bram G. Sengers ◽  
Rahul S. Tare ◽  
Franchesca D. Houghton
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Articular Chondrocytes ◽  
Embryonic Stem ◽  
Cartilage Tissue ◽  
Human Articular Cartilage ◽  
3D Scaffold ◽  
Tissue Constructs

AbstractArticular cartilage functions as a shock absorber and facilitates the free movement of joints. Currently, there are no therapeutic drugs that promote the healing of damaged articular cartilage. Limitations associated with the two clinically relevant cell populations, human articular chondrocytes and mesenchymal stem cells, necessitate finding an alternative cell source for cartilage repair. Human embryonic stem cells (hESCs) provide a readily accessible population of self-renewing, pluripotent cells with perceived immunoprivileged properties for cartilage generation. We have developed a robust method to generate 3D, scaffold-free, hyaline cartilage tissue constructs from hESCs that are composed of numerous chondrocytes in lacunae, embedded in an extracellular matrix containing Type II collagen, sulphated glycosaminoglycans and Aggrecan. The elastic (Young’s) modulus of the hESC-derived cartilage tissue constructs (0.91 ± 0.08 MPa) was comparable to full-thickness human articular cartilage (0.87 ± 0.09 MPa). Moreover, we have successfully scaled up the size of the scaffold-free, 3D hESC-derived cartilage tissue constructs to between 4.5 mm and 6 mm, thus enhancing their suitability for clinical application.

Rat embryonic stem cells created

Nature ◽  
2008 ◽  
Author(s):  
Monya Baker
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem
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