Total Polyphenol Content and Antiproliferative Activity of Green Tea Extracts Collected from Romanian Pharmaceuticals Market

2017 ◽  
Vol 68 (6) ◽  
pp. 1174-1176
Author(s):  
Corina Danciu ◽  
Ersilia Alexa ◽  
Stefana Avram ◽  
Daliana Minda ◽  
Ioana Zinuca Pavel ◽  
...  
Keyword(s):  
Cell Lines ◽  
Green Tea ◽  
Melanoma Cell ◽  
Proliferative Activity ◽  
Proliferative Potential ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Dose Dependent ◽  
Melanoma Cell Lines

Total polyphenol content and anti proliferative activity of five green tea extracts available on the Romanian pharmaceuticals market was analyzed. Results show dose-dependent anti proliferative activity of the selected samples against B164A5 mouse melanoma and A375 human melanoma cell lines. Sample 3, the richest sample in polyphenols showed the highest anti proliferative potential against the screened melanoma cell lines.

Anti-proliferative activity of haloperidol in B16 mouse and human SK-MEL-28 melanoma cell lines

2005 ◽  
Author(s):  
Jardena Nordenberg ◽  
Israela Perlmutter ◽  
Gad Lavie ◽  
Einat Beery ◽  
Orit Uziel ◽  
...  
Keyword(s):  
Cell Lines ◽  
Melanoma Cell ◽  
Proliferative Activity ◽  
Melanoma Cell Lines

Effect of (−)-epigallocatechin gallate, the main constituent of green tea, on lung metastasis with mouse B16 melanoma cell lines

1992 ◽  
Vol 65 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Shun'ichiro Taniguchi ◽  
Hirota Fujiki ◽  
Hiroaki Kobayashi ◽  
Hiroki Go ◽  
Kenji Miyado ◽  
...  
Keyword(s):  
Cell Lines ◽  
Green Tea ◽  
Lung Metastasis ◽  
Melanoma Cell ◽  
Epigallocatechin Gallate ◽  
B16 Melanoma ◽  
Main Constituent ◽  
B16 Melanoma Cell ◽  
Melanoma Cell Lines

scholarly journals Total Polyphenol Content in Green Tea (Camellia Sinensis) Using Maceration Extraction with Comparison of Ethanol – Water Solvent

2021 ◽  
Vol 1 (1) ◽  
pp. 16-23
Author(s):  
Dwi Evitasari ◽  
Erna Susanti
Keyword(s):  
Green Tea ◽  
Ethanol Concentration ◽  
Green Tea Extract ◽  
Solvent Concentration ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Water Solvent ◽  
Significant Difference ◽  
Tea Extract

Green tea is a kind of plant that have an active polyphenol substance. Polyphenol is a group of compounds that has a role as a natural antioxidant. Solvent comparison used affects the extracted polyphenol content. This study aims to compare the total polyphenol content in green tea from maceration method with solvent concentration variation of 30%, 50%, and 70%. In this research, green tea is extracted by using the maceration method with ethanol – water solvent concentration of 30%, 50%, and 70%. The total polyphenol content determination in green tea was conducted by using the Follin-Ciocalteu method by UV-Vis spectrophotometre, and using gallic acid as a standard. According to this research, the yield calculation of ethanol extract in green tea with the ethanol concentration of 30%, 50%, and 70% are 41.220%; 39.979 %; and 47.971 %. The total polyphenol content test of green tea with the ethanol concentration of 30%, 50%, and 70% are 11.294 mg GAE/100 g; 16.377 mg GAE/100g; and 15.640 mg GAE/100 g. The anova statistical analysis result shows that there is a significant difference of total polyphenol content of green tea extract with ethanol concentration 30% and 50%, also there is no significant difference in the ethanol concentration of 70%. The highest result of total polyphenol of green tea extract with the ethanol concentration of 50%. It is suggested to conduct a further study on the type of the polyphenol substance in green tea.  

Simple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beverages

2020 ◽  
Vol 25 (2) ◽  
pp. 255-263
Author(s):  
Mochammad Amrun Hidayat ◽  
Diah Ayu Maharani ◽  
Djoko Agus Purwanto ◽  
Bambang Kuswandi ◽  
Mochammad Yuwono
Keyword(s):  
Green Tea ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Total Polyphenol Content

The antiradical, anti-inflammatory and anti-genotoxic potential of herbal preparation Chlamyfin

2013 ◽  
Vol 32 (1) ◽  
pp. 227 ◽  
Author(s):  
Andreja Leskovac ◽  
Gordana Joksic ◽  
Igor Pasti ◽  
Tamara Lazarevic-Pasti ◽  
Branislav Nastasijevic ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Proliferative Potential ◽  
Scavenging Activity ◽  
Herbal Preparation ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Reducing Ability ◽  
Anti Inflammatory

Herbal preparation Chlamyfin was investigated for its total polyphenol content, 2,2-diphenyl-1- picrylhydrazyl (DPPH) scavenging activity, and anti-inflammatory and genotoxic properties. A high total polyphenol content provided evidence of high DPPH radical scavenging activity (IC50 = 4.96 ± 0.23 μg/ml). Analysis of the electrochemical behavior of Chlamyfin indicated high reducing ability, i.e., high antioxidant capacity, in agreement with the DPPH test. Analysis of myeloperoxidase (MPO) inhibition by Chlamyfin suggested anti-inflammatory action (IC50 values of 5.40 μg/ml and 4.45 μg/ml for an incubation time of 10 and 30 min, respectively). For genotoxic assessment, oxidative stress was induced by irradiation of peripheral whole blood with gamma-radiation in vitro. In the presence of Chlamyfin, reduced incidence of micronuclei without disturbance to the proliferative potential of cells was evidenced in both irradiated and unirradiated samples, indicating its genoprotective properties. It was shown that Chlamyfin, in addition to its bactericidal effect, also possesses strong antioxidant, anti-inflammatory and anti-genotoxic properties.

Braf Mutations Are Associated With High Levels of Phosphorylated RKIP in Melanoma Cell Lines: Potential Prognostic Significance

2011 ◽  
Vol 2 (2) ◽  
pp. 189-194 ◽  
Author(s):  
Sara Huerta-Yepez ◽  
S. Ekmekcioglu ◽  
C. M. Rivera-Pazos ◽  
G. Antonio-Andres ◽  
Mario I. Vega ◽  
...  
Keyword(s):  
Cell Lines ◽  
Melanoma Cell ◽  
Prognostic Significance ◽  
Braf Mutations ◽  
Melanoma Cell Lines

Assessment of Antimicrobial Activity and total Polyphenol Content of Rosa multiflora and Rosa wichuraiana

2019 ◽  
Vol 10 (04) ◽  
pp. 646-650
Author(s):  
Hyeusoo Kim ◽  
Kyeong Won Yun
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Fraction ◽  
Rosa Multiflora ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Water Fraction ◽  
Total Polyphenol Content ◽  
Rosa Species

The fruit of Rosa multiflora has been used as traditional herbal medicine in Asian countries. The present investigation was undertaken to study the antimicrobial activity and total polyphenol content of hexane, ether, ethyl acetate, water fraction of methanol extract of fruit and flower from Rosa multiflora and Rosa wichuraiana. Antimicrobial activity of the mentioned fractions against 3 gram-positive and 4 gram-negative bacteria using disk diffussion method. The measurement of minimal inhibition concentration (MIC) showed that the ethyl acetate fraction of the two Rosa species is the most effective against the tested bacteria. The total polyphenol content of ethyl acetate fraction of the two Rosa species is higher than the other fractions. The results indicate the antimicrobial activity was related with the total polyphenol content and the fruit and flower of the two Rosa species can be considered as a natural source of antimicrobial agents.

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