Immunohistochemistry Detection Apoptosis Related with ORF Virus Infection in Sheep Based on Caspase 3 Detection from Selected Farms in Basrah

2022 ◽  
Vol 16 (1) ◽  
Keyword(s):  
Virus Infection ◽  
Caspase 3 ◽  
Orf Virus

The P-MAPA immunomodulator partially prevents apoptosis induced by Zika virus infection in THP-1 cells

2020 ◽  
Vol 21 ◽  
Author(s):  
Morganna C. Lima ◽  
Elisa A. N. Azevedo ◽  
Clarice N. L. de Morais ◽  
Larissa I. O. de Sousa ◽  
Bruno M. Carvalho ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Virus Infection ◽  
Virus Replication ◽  
Zika Virus ◽  
Mammalian Cells ◽  
Caspase 3 ◽  
Neurological Complications ◽  
Zika Virus Infection

Background: Zika virus is an emerging arbovirus of global importance. ZIKV infection is associated with a range of neurological complications such as the Congenital Zika Syndrome and Guillain Barré Syndrome. Despite the magnitude of recent outbreaks, there is no specific therapy to prevent or to alleviate disease pathology. Objective: To investigate the role of P-MAPA immunomodulator in Zika-infected THP-1 cells. Methods: THP-1 cells were subjected at Zika virus infection (Multiplicity of Infection = 0.5) followed by treatment with P-MAPA for until 96 hours post-infection. After that, the cell death was analyzed by annexin+/ PI+ and caspase 3/ 7+ staining by flow cytometry. In addition, the virus replication and cell proliferation were accessed by RT-qPCR and Ki67 staining, respectively. Results: We demonstrate that P-MAPA in vitro treatment significantly reduces Zika virus-induced cell death and caspase-3/7 activation on THP-1 infected cells, albeit it has no role in virus replication and cell proliferation. Conclusions: Our study reveals that P-MAPA seems to be a satisfactory alternative to inhibits the effects of Zika virus infection in mammalian cells.

scholarly journals Virus infection induces proteolytic processing of IL-18 in human macrophages via caspase-1 and caspase-3 activation

2001 ◽  
Vol 31 (3) ◽  
pp. 726-733 ◽  
Author(s):  
Jaana Pirhonen ◽  
Timo Sareneva ◽  
Ilkka Julkunen ◽  
Sampsa Matikainen
Keyword(s):  
Virus Infection ◽  
Caspase 3 ◽  
Proteolytic Processing ◽  
Human Macrophages ◽  
Caspase 1

scholarly journals Orf virus infection after Eid al-Adha

IDCases ◽  
2020 ◽  
Vol 21 ◽  
pp. e00854
Author(s):  
Ashley Vellucci ◽  
Melina Manolas ◽  
Sarah Jin ◽  
John Dwyer ◽  
Garrett Vick ◽  
...  
Keyword(s):  
Virus Infection ◽  
Orf Virus

scholarly journals Viral Vascular Endothelial Growth Factor Plays a Critical Role in Orf Virus Infection

2000 ◽  
Vol 74 (22) ◽  
pp. 10699-10706 ◽  
Author(s):  
Loreen J. Savory ◽  
Steven A. Stacker ◽  
Stephen B. Fleming ◽  
Brian E. Niven ◽  
Andrew A. Mercer
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Virus Infection ◽  
Vascular Endothelial Cells ◽  
Critical Role ◽  
Skin Lesions ◽  
Orf Virus ◽  
Vegf Receptor ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

ABSTRACT Infection by the parapoxvirus orf virus causes proliferative skin lesions in which extensive capillary proliferation and dilation are prominent histological features. This infective phenotype may be linked to a unique virus-encoded factor, a distinctive new member of the vascular endothelial growth factor (VEGF) family of molecules. We constructed a recombinant orf virus in which the VEGF-like gene was disrupted and show that inactivation of this gene resulted in the loss of three VEGF activities expressed by the parent virus: mitogenesis of vascular endothelial cells, induction of vascular permeability, and activation of VEGF receptor 2. We used the recombinant orf virus to assess the contribution of the viral VEGF to the vascular response seen during orf virus infection of skin. Our results demonstrate that the viral VEGF, while recognizing a unique profile of the known VEGF receptors (receptor 2 and neuropilin 1), is able to stimulate a striking proliferation of blood vessels in the dermis underlying the site of infection. Furthermore, the data demonstrate that the viral VEGF participates in promoting a distinctive pattern of epidermal proliferation. Loss of a functional viral VEGF resulted in lesions with markedly reduced clinical indications of infection. However, viral replication in the early stages of infection was not impaired, and only at later times did it appear that replication of the recombinant virus might be reduced.

Virological and clinico-pathological features of orf virus infection in experimentally infected rabbits and mice

2011 ◽  
Vol 50 (1) ◽  
pp. 56-62 ◽  
Author(s):  
J.F. Cargnelutti ◽  
E.K. Masuda ◽  
M. Martins ◽  
D.G. Diel ◽  
D.L. Rock ◽  
...  
Keyword(s):  
Virus Infection ◽  
Orf Virus ◽  
Pathological Features

Inflammatory nodules around the axilla: an uncommon localization of orf virus infection

2009 ◽  
Vol 34 (2) ◽  
pp. 240-242 ◽  
Author(s):  
B Weide ◽  
G. Metzler ◽  
T. K. Eigentler ◽  
B. Fehrenbacher ◽  
K. Sönnichsen ◽  
...  
Keyword(s):  
Virus Infection ◽  
Orf Virus

ORF virus infection of workers in the meat industry

1984 ◽  
Vol 8 (1) ◽  
pp. 95-96
Keyword(s):  
Virus Infection ◽  
Orf Virus ◽  
Meat Industry

scholarly journals Orf virus infection in Alaskan mountain goats, Dall’s sheep, muskoxen, caribou and Sitka black-tailed deer

2018 ◽  
Vol 60 (1) ◽  
Author(s):  
Morten Tryland ◽  
Kimberlee Beth Beckmen ◽  
Kathleen Ann Burek-Huntington ◽  
Eva Marie Breines ◽  
Joern Klein
Keyword(s):  
Virus Infection ◽  
Orf Virus ◽  
Mountain Goats ◽  
Dall’S Sheep
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