Matching method for data sequences from on-line calibration of laser displacement meter

In the past few years on-line digital television frame store devices coupled to computers have been employed to attempt to measure the microscope parameters of defocus and astigmatism. The ultimate goal of such tasks is to fully adjust the operating parameters of the microscope and obtain an optimum image for viewing in terms of its information content. The initial approach to this problem, for high resolution TEM imaging, was to obtain the power spectrum from the Fourier transform of an image, find the contrast transfer function oscillation maxima, and subsequently correct the image. This technique requires a fast computer, a direct memory access device and even an array processor to accomplish these tasks on limited size arrays in a few seconds per image. It is not clear that the power spectrum could be used for more than defocus correction since the correction of astigmatism is a formidable problem of pattern recognition.

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Image registration with a computer driven S.T.E.M.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100107630 ◽

1978 ◽

Vol 36 (1) ◽

pp. 98-99

Author(s):

A.M.H. Schepman ◽

J.A.P. van der Voort ◽

J.E. Mellema

Keyword(s):

Spot Size ◽

Scanning Transmission Electron Microscope ◽

Small Computer ◽

Structural Studies ◽

Area Of Interest ◽

Transmission Electron ◽

Scanning Transmission ◽

Specimen Area ◽

On Line ◽

Minimum Distances

A Scanning Transmission Electron Microscope (STEM) was coupled to a small computer. The system (see Fig. 1) has been built using a Philips EM400, equipped with a scanning attachment and a DEC PDP11/34 computer with 34K memory. The gun (Fig. 2) consists of a continuously renewed tip of radius 0.2 to 0.4 μm of a tungsten wire heated just below its melting point by a focussed laser beam (1). On-line operation procedures were developped aiming at the reduction of the amount of radiation of the specimen area of interest, while selecting the various imaging parameters and upon registration of the information content. Whereas the theoretical limiting spot size is 0.75 nm (2), routine resolution checks showed minimum distances in the order 1.2 to 1.5 nm between corresponding intensity maxima in successive scans. This value is sufficient for structural studies of regular biological material to test the performance of STEM over high resolution CTEM.

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Design and calibration of an IVEM for general 3-dimensional imaging of non-diffracting materials

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100129838 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1040-1041

Author(s):

Neil Rowlands ◽

Jeff Price ◽

Michael Kersker ◽

Seichi Suzuki ◽

Steve Young ◽

...

Keyword(s):

3D Imaging ◽

Tomographic Reconstruction ◽

Three Dimensional ◽

3 Dimensional ◽

3D Microstructure ◽

Image Translation ◽

Digital Correlation ◽

On Line ◽

Mechanical Stage ◽

Projection Angle

Three-dimensional (3D) microstructure visualization on the electron microscope requires that the sample be tilted to different positions to collect a series of projections. This tilting should be performed rapidly for on-line stereo viewing and precisely for off-line tomographic reconstruction. Usually a projection series is collected using mechanical stage tilt alone. The stereo pairs must be viewed off-line and the 60 to 120 tomographic projections must be aligned with fiduciary markers or digital correlation methods. The delay in viewing stereo pairs and the alignment problems in tomographic reconstruction could be eliminated or improved by tilting the beam if such tilt could be accomplished without image translation.A microscope capable of beam tilt with simultaneous image shift to eliminate tilt-induced translation has been investigated for 3D imaging of thick (1 μm) biologic specimens. By tilting the beam above and through the specimen and bringing it back below the specimen, a brightfield image with a projection angle corresponding to the beam tilt angle can be recorded (Fig. 1a).

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