scholarly journals Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

10.3791/187 ◽  
2007 ◽  
Author(s):  
Souichi Ogata ◽  
Ken W.Y. Cho
Keyword(s):  
Cell Adhesion ◽  
Xenopus Laevis ◽  
Adhesion Assay ◽  
Animal Pole ◽  
Cell Adhesion Assay ◽  
A Cell ◽  
Xenopus Laevis Embryos

Efficient synthesis of novel RGD based peptides and the conjugation of the pyrazine moiety to their N-terminus

2019 ◽  
Vol 43 (6) ◽  
pp. 2702-2709
Author(s):  
Fatima Hamdan ◽  
Zahra Bigdeli ◽  
Saeed Balalaie ◽  
Norbert Sewald ◽  
Carmela Michalek
Keyword(s):  
Cell Adhesion ◽  
Adhesion Assay ◽  
Efficient Synthesis ◽  
Cell Adhesion Assay ◽  
N Terminus ◽  
A Cell

Novel RGD based peptides (RGDFAKLF and RGDNGRG) were designed and synthesized and were later coupled to the pyrazine moiety at the N-terminus. The IC50 values from the in vitro study of the target peptides using a cell adhesion assay indicated the essential impact of the existence of the pyrazine moiety. Meanwhile, peptide 4 exhibited the best IC50 value.

A fluorometric approach to the quantitation of cell number with application to a cell adhesion assay

1988 ◽  
Vol 110 (1) ◽  
pp. 93-100 ◽  
Author(s):  
David M. Lewinsohn ◽  
Brian J. Nickoloff ◽  
Eugene C. Butcher
Keyword(s):  
Cell Adhesion ◽  
Cell Number ◽  
Adhesion Assay ◽  
Cell Adhesion Assay ◽  
A Cell

Screening for novel cell adhesive regions in bovine Achilles tendon collagen peptides

2014 ◽  
Vol 92 (1) ◽  
pp. 9-22 ◽  
Author(s):  
Pradipta Banerjee ◽  
Alka Mehta ◽  
C. Shanthi
Keyword(s):  
Cell Adhesion ◽  
Docking Studies ◽  
Exchange Chromatography ◽  
Adhesion Assay ◽  
Structural Protein ◽  
Cell Adherence ◽  
Collagen Peptides ◽  
Adhesion Ability ◽  
A Cell ◽  
Hydrophilic Residues

Collagen, a major structural protein of the ECM, is known for its high cell adherence capacity. This study was conducted to identify regions in collagen that harbour such bioactivity. Collagen from tendon was hydrolysed and the peptides fractionated using ion-exchange chromatography (IEC). Isolated peptide fractions were coated onto disposable dishes and screened for cell adherence and proliferative abilities. Active IEC fractions were further purified by chromatography, and two peptides, C2 and E1 with cell adhesion ability, were isolated. A cell adhesion assay done with different amounts of C2 coated onto disposable dishes revealed the maximum adhesion to be 94.6%, compared with 80% for collagen coated dishes and an optimum peptide coating density of 0.507 nmoles per cm2 area of the dish. Growth of cells on C2, collagen, and E1 revealed a similar pattern and a reduction in the doubling time compared with cells grown on uncoated dishes. C2 had a mass of 2.046 kDa with 22 residues, and sequence analysis revealed a higher percentage occurrence of hydrophilic residues compared with other regions in collagen. Docking studies revealed GDDGEA in C2 as the probable site of interaction with integrins α2β1 and α1β1, and stability studies proved C2 to be mostly protease-resistant.

Development of a high throughput Pseudomonas aeruginosa epithelial cell adhesion assay

2003 ◽  
Vol 52 (3) ◽  
pp. 361-366 ◽  
Author(s):  
Britta Swanson ◽  
Richard Savel ◽  
Frank Szoka ◽  
Teiji Sawa ◽  
Jeanine Wiener-Kronish
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
High Throughput ◽  
Adhesion Assay ◽  
Cell Adhesion Assay

scholarly journals Quantitative cell-adhesion assay for Clostridium difficile cytotoxin

1987 ◽  
Vol 24 (3) ◽  
pp. 197-203
Author(s):  
B. C. Mayall ◽  
C. Edmonds ◽  
G. E. Griffin
Keyword(s):  
Cell Adhesion ◽  
Clostridium Difficile ◽  
Adhesion Assay ◽  
Cell Adhesion Assay

Live cell adhesion assay with attenuated total reflection infrared spectroscopy

2007 ◽  
Vol 2 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Martin Schmidt ◽  
Tobias Wolfram ◽  
Monika Rumpler ◽  
Carl P. Tripp ◽  
Michael Grunze
Keyword(s):  
Cell Adhesion ◽  
Infrared Spectroscopy ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Live Cell ◽  
Adhesion Assay ◽  
Cell Adhesion Assay

Simple colorimetric cell-cell adhesion assay using MTT-stained leukemia cells

1993 ◽  
Vol 164 (2) ◽  
pp. 255-261 ◽  
Author(s):  
Ichiro Miki ◽  
Naomi Ishihara ◽  
Masanari Otoshi ◽  
Hiroshi Kase
Keyword(s):  
Cell Adhesion ◽  
Leukemia Cells ◽  
Adhesion Assay ◽  
Cell Adhesion Assay ◽  
Cell Cell
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