A centrifugation cell adhesion assay for high-throughput screening of biomaterial surfaces

2003 ◽  
Vol 67A (1) ◽  
pp. 328-333 ◽  
Author(s):  
Catherine D. Reyes ◽  
Andrés J. García
Keyword(s):  
Cell Adhesion ◽  
High Throughput ◽  
High Throughput Screening ◽  
Adhesion Assay ◽  
Cell Adhesion Assay ◽  
Biomaterial Surfaces

Development of a high throughput Pseudomonas aeruginosa epithelial cell adhesion assay

2003 ◽  
Vol 52 (3) ◽  
pp. 361-366 ◽  
Author(s):  
Britta Swanson ◽  
Richard Savel ◽  
Frank Szoka ◽  
Teiji Sawa ◽  
Jeanine Wiener-Kronish
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
High Throughput ◽  
Adhesion Assay ◽  
Cell Adhesion Assay

scholarly journals High-throughput screening and rational design of biofunctionalized surfaces with optimized biocompatibility and antimicrobial activity

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhou Fang ◽  
Junjian Chen ◽  
Ye Zhu ◽  
Guansong Hu ◽  
Haoqian Xin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
High Throughput ◽  
High Throughput Screening ◽  
Rational Design ◽  
Click Reaction ◽  
Reaction Times ◽  
Great Promise ◽  
Biomaterial Surfaces

AbstractPeptides are widely used for surface modification to develop improved implants, such as cell adhesion RGD peptide and antimicrobial peptide (AMP). However, it is a daunting challenge to identify an optimized condition with the two peptides showing their intended activities and the parameters for reaching such a condition. Herein, we develop a high-throughput strategy, preparing titanium (Ti) surfaces with a gradient in peptide density by click reaction as a platform, to screen the positions with desired functions. Such positions are corresponding to optimized molecular parameters (peptide densities/ratios) and associated preparation parameters (reaction times/reactant concentrations). These parameters are then extracted to prepare nongradient mono- and dual-peptide functionalized Ti surfaces with desired biocompatibility or/and antimicrobial activity in vitro and in vivo. We also demonstrate this strategy could be extended to other materials. Here, we show that the high-throughput versatile strategy holds great promise for rational design and preparation of functional biomaterial surfaces.

High-throughput screening of cellular features using high-resolution light-microscopy; Application for profiling drug effects on cell adhesion

2007 ◽  
Vol 158 (2) ◽  
pp. 233-243 ◽  
Author(s):  
Yael Paran ◽  
Micha Ilan ◽  
Yoel Kashman ◽  
Sofee Goldstein ◽  
Yuvalal Liron ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
High Resolution ◽  
Light Microscopy ◽  
High Throughput ◽  
High Throughput Screening ◽  
Drug Effects

scholarly journals Quantitative cell-adhesion assay for Clostridium difficile cytotoxin

1987 ◽  
Vol 24 (3) ◽  
pp. 197-203
Author(s):  
B. C. Mayall ◽  
C. Edmonds ◽  
G. E. Griffin
Keyword(s):  
Cell Adhesion ◽  
Clostridium Difficile ◽  
Adhesion Assay ◽  
Cell Adhesion Assay

Live cell adhesion assay with attenuated total reflection infrared spectroscopy

2007 ◽  
Vol 2 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Martin Schmidt ◽  
Tobias Wolfram ◽  
Monika Rumpler ◽  
Carl P. Tripp ◽  
Michael Grunze
Keyword(s):  
Cell Adhesion ◽  
Infrared Spectroscopy ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Live Cell ◽  
Adhesion Assay ◽  
Cell Adhesion Assay

Simple colorimetric cell-cell adhesion assay using MTT-stained leukemia cells

1993 ◽  
Vol 164 (2) ◽  
pp. 255-261 ◽  
Author(s):  
Ichiro Miki ◽  
Naomi Ishihara ◽  
Masanari Otoshi ◽  
Hiroshi Kase
Keyword(s):  
Cell Adhesion ◽  
Leukemia Cells ◽  
Adhesion Assay ◽  
Cell Adhesion Assay ◽  
Cell Cell

scholarly journals Detection of Enteropathogenic Esherichia coli (EPEC) by Serotyping and Cell Adhesion Assay among Children in North-Eastern Peninsular Malaysia: A Hospital Based Study

1970 ◽  
Vol 2 (2) ◽  
pp. 40-43
Author(s):  
J Ashraful Haq ◽  
Hin Choon Li ◽  
Rosliza Abdur Rahman
Keyword(s):  
Escherichia Coli ◽  
Cell Adhesion ◽  
Hela Cell ◽  
Peninsular Malaysia ◽  
Watery Diarrhea ◽  
Adhesion Assay ◽  
Childhood Diarrhea ◽  
E Coli ◽  
Cell Adhesion Assay ◽  
North Eastern

Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in children below 5 years of age in the developing countries. The present study investigated the role of EPEC in childhood diarrhea among the patients attending a university hospital in north-eastern peninsular Malaysia by serotyping and cell adhesion assay using HEp-2 and HeLa cells. A total of 60 stools or rectal swabs from watery diarrhea cases and 16 age matched healthy controls were examined. EPEC were isolated from 14 (23.3%) diarrhea cases and from 1 (9.1%) control by serotyping. Of the 14 EPEC strains, the predominant strain was 0125: K70 (28.5%). Cell adhesion assay detected 26.6% and 30.0% adherent Escherichia coli (E. coli) in diarrhea cases by HEp-2 and HeLa system respectively. Three adherence patterns were noted namely localized, diffuse and aggregative patterns. About 81- 88% of isolated E. coli exhibited diffuse adherence pattern by HEp-2 and HeLa cell assay respectively. About 43-44% E. coli exhibiting positive cell adherence phenotype with HEp-2 and HeLa cell assays tested negative with EPEC antisera. The findings indicate that EPEC is an important cause of childhood diarrhea in north-eastern peninsular Malaysia and cell adhesion assay is more sensitive than serotyping for detection of diarrheogenic E. coli. Ibrahim Med. Coll. J. 2008; 2(2): 40-43 Key Words: EPEC, diarrhea, serotyping, Malaysia doi: 10.3329/imcj.v2i2.2935

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