scholarly journals Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

10.3791/3273 ◽  
2011 ◽  
Author(s):  
Xuejun H. Parsons ◽  
Yang D. Teng ◽  
James F. Parsons ◽  
Evan Y. Snyder ◽  
David B. Smotrich ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Small Molecule ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Neuronal Progenitors

scholarly journals Small Molecule-Mediated Directed Differentiation of Human Embryonic Stem Cells Toward Ventricular Cardiomyocytes

2013 ◽  
Vol 3 (1) ◽  
pp. 18-31 ◽  
Author(s):  
Ioannis Karakikes ◽  
Grant D. Senyei ◽  
Jens Hansen ◽  
Chi-Wing Kong ◽  
Evren U. Azeloglu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Small Molecule ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Directed Differentiation ◽  
Ventricular Cardiomyocytes

scholarly journals Enhanced non-viral gene delivery to human embryonic stem cells via small molecule-mediated transient alteration of the cell structure

2014 ◽  
Vol 2 (46) ◽  
pp. 8098-8105 ◽  
Author(s):  
Jonathan Yen ◽  
Lichen Yin ◽  
Jianjun Cheng
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Delivery ◽  
Small Molecule ◽  
Human Embryonic Stem Cells ◽  
Cell Structure ◽  
Embryonic Stem ◽  
Viral Gene ◽  
Viral Gene Delivery ◽  
Membrane Exposure

Y-27632 enhances the transfection efficiencies of various polyplexes or lipoplexes in hESCs via increased membrane exposure through transient spreading of the cells.

scholarly journals An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells

2011 ◽  
Vol 2 (1) ◽  
Author(s):  
Hideaki Tsutsui ◽  
Bahram Valamehr ◽  
Antreas Hindoyan ◽  
Rong Qiao ◽  
Xianting Ding ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Small Molecule ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Small Molecule Inhibitor ◽  
Molecule Inhibitor ◽  
Term Maintenance

scholarly journals Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs. Small Molecule-Based Approaches

2019 ◽  
Vol 2019 ◽  
pp. 1-18 ◽  
Author(s):  
Divya S. Varghese ◽  
Thilina Thejinda Alawathugoda ◽  
Suraiya A. Ansari
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Growth Factor ◽  
Small Molecule ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
The Body ◽  
Fine Tuning ◽  
Specific Marker ◽  
Hepatocyte Differentiation

Human embryonic stem cells (hESCs) are being utilized in diverse areas of studies such as development and disease modeling, cell replacement therapy, or drug toxicity testing because of their potential to be differentiated into any cell type in the body. The directed differentiation of hESCs into hepatocytes could provide an invaluable source of liver cells for various liver-based applications. Therefore, several protocols have been established in the past for hESC-hepatocyte differentiation based on the knowledge of signaling pathways and growth factors involved in different stages of embryonic hepatogenesis. Although successful derivation of hepatocytes has been achieved through these protocols, the efficiency is not always ideal. Herein, we have tested several combinations of published protocols, for example, growth factor vs. small molecule and different time durations of treatment for definitive endoderm (DE) induction and further hepatocyte differentiation to develop an efficient DE induction and hepatocyte differentiation in a highly reproducible manner based on the stage-specific marker expression and functional analysis.

scholarly journals Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

10.3791/3274 ◽  
2011 ◽  
Author(s):  
Xuejun H. Parsons ◽  
Yang D. Teng ◽  
James F. Parsons ◽  
Evan Y. Snyder ◽  
David B. Smotrich ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Small Molecule ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem
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