Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the <em>N</em><sup>6</sup>-threonylcarbamoyladenosine TcdA Function

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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Identification of Fibrinogen Derivatives in Plasma Samples

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649400 ◽

1972 ◽

Vol 27 (03) ◽

pp. 610-618 ◽

Cited By ~ 14

Author(s):

H Graeff ◽

R von Hugo

Keyword(s):

Molecular Weight ◽

Gel Electrophoresis ◽

Gel Chromatography ◽

Agarose Gel ◽

Plasma Samples ◽

Methodological Study ◽

Parent Molecule ◽

Electrophoretic Mobilities ◽

Fibrin Monomer

SummaryThe observation of fibrinogen derivatives with a molecular weight higher than the parent molecule in human cases of DIC initiated the present methodological study. These derivatives were identified by the following methods : 2.5 M β-alanine precipitation of the plasma samples, PAA gel electrophoresis, intra gel immunoprecipitation and agarose gel chromatography. In the plasma of a patient with severe eclampsia and laboratory signs of DIC two derivatives with a molecular weight higher than that of fibrinogen were identified according to their relative electrophoretic mobilities: 0.18 and 0.28 × 10−5 cm2/V × sec (fibrinogen: 0.43 × 10−5 cm2/V × sec). Electrophoretic studies in the presence of 5 M urea indicated that the 0.28 derivative is a complex probably formed by fibrinogen and a fibrin monomer.

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The Preparation and Evaluation of a Standardized Fibrin Plate for the Assessment of Fibrinolytic Activity

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654135 ◽

1970 ◽

Vol 23 (02) ◽

pp. 202-210 ◽

Cited By ~ 13

Author(s):

R Bishop ◽

H Ekert ◽

G Gilchrist ◽

E Shanbrom ◽

L Fekete

Keyword(s):

Test Specimen ◽

Fibrinolytic Activity ◽

Clinical Laboratory ◽

Fibrin Clot ◽

Fibrinolytic Enzyme ◽

Agarose Gel ◽

Fibrin Plate ◽

Percent Concentration ◽

Human Plasminogen ◽

Preparation And Evaluation

SummaryA new fibrin plate technic for evaluating components of the fibrinolytic system has been developed. It provides quick, accurate, and easily interpreted results for the fibrinolytic profile. The standardized human plasminogen-free fibrin plates can be produced in bulk and stored for prolonged periods of time. A test specimen placed in a well punched in the buffered agarose gel diffuses into the agar and lyses the fibrin clot, forming a clear reaction zone. The zone diameter is directly proportional to the log of the percent concentration of available fibrinolytic enzyme in the specimen. The plates may be used to quantitate total plasminogen, and estimate available plasmin and active plasmin. A good correlation between results obtained using these fibrin plates and caseinolytic methods was found. Performance and interpretation of tests of fibrinolysis done on these new fibrin plates indicate that it may be the most sensitive technic available for clinical laboratory work.

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