scholarly journals Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

10.3791/57209 ◽  
2018 ◽  
Author(s):  
Lei Wei ◽  
Jesse G. Meyer ◽  
Birgit Schilling
Keyword(s):  
Mass Spectrometry ◽  
Specific Protein ◽  
Lysine Acetylation ◽  
Site Specific ◽  
Data Independent Acquisition ◽  
Protein Lysine Acetylation ◽  
Using Data

The challenge of detecting modifications on proteins

2020 ◽  
Vol 64 (1) ◽  
pp. 135-153 ◽  
Author(s):  
Lauren Elizabeth Smith ◽  
Adelina Rogowska-Wrzesinska
Keyword(s):  
Mass Spectrometry ◽  
Protein Function ◽  
Chemical Properties ◽  
Lysine Acetylation ◽  
Mass Determination ◽  
Post Translational Modifications ◽  
Site Specific ◽  
The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

scholarly journals Proteomic comparison of osteoarthritic and reference human menisci using data-independent acquisition mass spectrometry

2020 ◽  
Vol 28 (8) ◽  
pp. 1092-1101
Author(s):  
E. Folkesson ◽  
A. Turkiewicz ◽  
N. Ali ◽  
M. Rydén ◽  
H.V. Hughes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Data Independent Acquisition ◽  
Using Data

scholarly journals Proteomic characterization of the normal human medial meniscus body using data‐independent acquisition mass spectrometry

2020 ◽  
Vol 38 (8) ◽  
pp. 1735-1745 ◽  
Author(s):  
Elin Folkesson ◽  
Aleksandra Turkiewicz ◽  
Martin Rydén ◽  
Harini Velocity Hughes ◽  
Neserin Ali ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Medial Meniscus ◽  
Data Independent Acquisition ◽  
Normal Human ◽  
Using Data

scholarly journals Sensitive and fast characterization of site-specific protein glycosylation with capillary electrophoresis coupled to mass spectrometry

Talanta ◽  
2018 ◽  
Vol 179 ◽  
pp. 22-27 ◽  
Author(s):  
Yanyan Qu ◽  
Liangliang Sun ◽  
Guijie Zhu ◽  
Zhenbin Zhang ◽  
Elizabeth H. Peuchen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Specific Protein ◽  
Protein Glycosylation ◽  
Site Specific

scholarly journals Differential quantification of isobaric phosphopeptides using data-independent acquisition mass spectrometry

2016 ◽  
Vol 12 (8) ◽  
pp. 2385-2388 ◽  
Author(s):  
Simone Sidoli ◽  
Rina Fujiwara ◽  
Katarzyna Kulej ◽  
Benjamin A. Garcia
Keyword(s):  
Mass Spectrometry ◽  
Data Independent Acquisition ◽  
Modified Peptides ◽  
Using Data

Data-independent acquisition (DIA) is here applied to determine unexplored phosphorylation events on isobarically modified peptides.

scholarly journals High-performance targeted mass spectrometry with precision data-independent acquisition reveals site-specific glycosylation macroheterogeneity

2016 ◽  
Vol 510 ◽  
pp. 106-113 ◽  
Author(s):  
K.Y. Benjamin Yeo ◽  
Panagiotis K. Chrysanthopoulos ◽  
Amanda S. Nouwens ◽  
Esteban Marcellin ◽  
Benjamin L. Schulz
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Precision Data ◽  
Site Specific ◽  
Data Independent Acquisition
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