Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis

10.3791/58751 ◽  
2018 ◽  
Author(s):  
Yu Zhang ◽  
Guan Wang ◽  
Jia Lu ◽  
Li-ming Xu ◽  
Jun-yu Xiong
Keyword(s):  
Escherichia Coli ◽  
Peritoneal Macrophage ◽  
Green Fluorescence Protein ◽  
Mouse Peritoneal Macrophage ◽  
Green Fluorescence ◽  
Enhanced Green Fluorescence Protein ◽  
Macrophage Phagocytosis ◽  
Fluorescence Protein

scholarly journals Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Min Xu ◽  
Yue-Ying Jiao ◽  
Yuan-Hui Fu ◽  
Nan Jiang ◽  
Yuan-Bo Zheng ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Respiratory Tract ◽  
Drug Screening ◽  
Green Fluorescence Protein ◽  
Respiratory Tract Disease ◽  
Green Fluorescence ◽  
Enhanced Green Fluorescence Protein ◽  
Fluorescence Protein ◽  
Syncytial Virus ◽  
Tract Disease

Human respiratory syncytial virus (RSV) is the single most important cause of lower respiratory tract disease in infants and young children and a major viral agent responsible for respiratory tract disease in immunosuppressed individuals and the elderly, but no vaccines and antiviral drugs are available. Herein the recombinant RSV (rRSV) encoding enhanced green fluorescence protein (EGFP, rRSV-EGFP) was constructed and the potential for screening anti-RSV drugs was investigated. The recombinant plasmid of pBRATm-rRSV-EGFP, containing T7 transcription cassette composed of T7 promoter, RSV antigenomic cDNA with EGFP gene, HDV ribozyme (δ), and T7 terminator in the order of 5′ to 3′, was constructed and cotransfected into BHK/T7-9 cells together with helper plasmids encoding N, P, L, and M2-1 gene, respectively. The rescued rRSV-EGFP was confirmed by increasing expression of EGFP over blind passages and by RT-PCR. rRSV-EGFP was comparable to the other two recombinant RSVs encoding red fluorescent protein (RFP, rRSV-RFP) or luciferase (Luc, rRSV-Luc) in the growth kinetic, and there was a difference in sensitivity between them for screening anti-RSV agents based on infection of HEp-2 cells. The EGFP-encoding rRSV has been constructed and rescued successfully and has the potential for high-throughput anti-RSV drug screening in vitro.

An enhanced green fluorescence protein (EGFP)-based reporter assay for quantitative detection of sporulation in Clostridium perfringens SM101

2019 ◽  
Vol 291 ◽  
pp. 144-150 ◽  
Author(s):  
Yuki Wakabayashi ◽  
Hirofumi Nariya ◽  
Mayo Yasugi ◽  
Tomomi Kuwahara ◽  
Mahfuzur R. Sarker ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Green Fluorescence Protein ◽  
Quantitative Detection ◽  
Reporter Assay ◽  
Green Fluorescence ◽  
Enhanced Green Fluorescence Protein ◽  
Fluorescence Protein

Therapeutic Effect of Dendritic Cells Loaded with a Fusion mRNA Encoding Tyrosinase-Related Protein 2 and Enhanced Green Fluorescence Protein on B16 Melanoma

Tumor Biology ◽  
2004 ◽  
Vol 25 (5-6) ◽  
pp. 252-257 ◽  
Author(s):  
Mikiko Fukui ◽  
Tomoko Nakano-Hashimoto ◽  
Kozue Okano ◽  
Yuichiro Maruta ◽  
Yutaka Suehiro ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Therapeutic Effect ◽  
Green Fluorescence Protein ◽  
B16 Melanoma ◽  
Related Protein ◽  
Green Fluorescence ◽  
Enhanced Green Fluorescence Protein ◽  
Fluorescence Protein ◽  
Tyrosinase Related Protein

Evaluation of an mRNA Lipofection Procedure for Human Dendritic Cells and Induction of Cytotoxic T Lymphocytes against Enhanced Green Fluorescence Protein

Tumor Biology ◽  
2003 ◽  
Vol 24 (6) ◽  
pp. 317-324 ◽  
Author(s):  
Kozue Okano ◽  
Mikiko Fukui ◽  
Yutaka Suehiro ◽  
Yuichiro Hamanaka ◽  
Kohzoh Imai ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Green Fluorescence Protein ◽  
Green Fluorescence ◽  
Enhanced Green Fluorescence Protein ◽  
Fluorescence Protein ◽  
Human Dendritic Cells

Quantification of frameshift mutations at a (CA)13 microsatellite with a novel, enhanced green fluorescence protein (EGFP) based plasmid

2001 ◽  
Vol 120 (5) ◽  
pp. A163 ◽  
Author(s):  
Christoph Gasche ◽  
Christina L. Chang ◽  
Jennifer Rhees ◽  
Christian Arnold ◽  
C. Richard Boland
Keyword(s):  
Green Fluorescence Protein ◽  
Green Fluorescence ◽  
Enhanced Green Fluorescence Protein ◽  
Frameshift Mutations ◽  
Fluorescence Protein
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