A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

10.3791/60197 ◽  
2019 ◽  
Author(s):  
Ana Marek ◽  
Christiane Schüler ◽  
María Satué ◽  
Barbara Haigl ◽  
Reinhold G. Erben
Keyword(s):  
Laser Capture Microdissection ◽  
High Quality ◽  
Fresh Frozen ◽  
Laser Capture

Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture Microdissection

2008 ◽  
pp. 35-45
Author(s):  
Dan-Arin Silasi ◽  
Ayesha B. Alvero ◽  
Jechiel Mor ◽  
Rui Chen ◽  
Han-Hsuan Fu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Laser Capture Microdissection ◽  
Fresh Frozen ◽  
Related Proteins ◽  
Laser Capture

Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture Microdissection

2008 ◽  
pp. 35-46
Author(s):  
Dan-Arin Silasi ◽  
Ayesha B. Alvero ◽  
Jechiel Mor ◽  
Rui Chen ◽  
Han-Hsuan Fu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Laser Capture Microdissection ◽  
Fresh Frozen ◽  
Related Proteins ◽  
Laser Capture

scholarly journals Tissue preparation for laser capture microdissection and RNA extraction from fresh frozen breast tissue

BioTechniques ◽  
2007 ◽  
Vol 43 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Mary Morrogh ◽  
Narciso Olvera ◽  
Faina Bogomolniy ◽  
Patrick I. Borgen ◽  
Tari A. King
Keyword(s):  
Laser Capture Microdissection ◽  
Breast Tissue ◽  
Rna Extraction ◽  
Tissue Preparation ◽  
Fresh Frozen ◽  
Laser Capture

scholarly journals High-Quality RNA from Cells Isolated by Laser Capture Microdissection

BioTechniques ◽  
2002 ◽  
Vol 33 (1) ◽  
pp. 176-179 ◽  
Author(s):  
A. Mikulowska-Mennis ◽  
T.B. Taylor ◽  
P. Vishnu ◽  
S.A. Michie ◽  
R. Raja ◽  
...  
Keyword(s):  
Laser Capture Microdissection ◽  
High Quality ◽  
Laser Capture

scholarly journals Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

10.3791/57762 ◽  
2018 ◽  
Author(s):  
Aaron A. May-Zhang ◽  
Karen K Deal ◽  
E. Michelle Southard-Smith
Keyword(s):  
Laser Capture Microdissection ◽  
Human Tissue ◽  
Enteric Ganglia ◽  
Fresh Frozen ◽  
Laser Capture

scholarly journals An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection

Plant Methods ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Muhammad Shahzad Anjam ◽  
Yvonne Ludwig ◽  
Frank Hochholdinger ◽  
Chisato Miyaura ◽  
Masaki Inada ◽  
...  
Keyword(s):  
Laser Capture Microdissection ◽  
High Quality ◽  
Laser Capture

scholarly journals High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection

2009 ◽  
Vol 10 (1) ◽  
pp. 69 ◽  
Author(s):  
Wei-Zhi Wang ◽  
Franziska M Oeschger ◽  
Sheena Lee ◽  
Zoltán Molnár
Keyword(s):  
Laser Capture Microdissection ◽  
Brain Regions ◽  
High Quality ◽  
Laser Capture

scholarly journals Laser Capture Microdissection optimization for high-quality RNA in mouse brain tissue

2021 ◽  
Author(s):  
Margareth Nogueira ◽  
Daiane CF Golbert ◽  
Richardson Leão
Keyword(s):  
Brain Tissue ◽  
Mouse Brain ◽  
Laser Capture Microdissection ◽  
Basolateral Amygdala ◽  
Single Cells ◽  
Heterogeneous Material ◽  
Anterior Cingulate ◽  
High Quality ◽  
Rna Integrity ◽  
Laser Capture

Laser Capture Microdissection (LCM) is a method that allows to select and dissecting specific structures, cell populations, or even single cells from different types of tissue to extract DNA, RNA, or proteins. It is easy to perform and precise, avoiding unwanted signals from irrelevant cells, because gene expression may be affected by a bulk of heterogeneous material in a sample. However, despite its efficiency, several steps can affect the sample RNA integrity. In comparison to DNA, RNA is a much more unstable molecule and represents a challenge in the LCM method. Here we describe an optimized protocol to provide good concentration and high-quality RNA in specific structures, such as Dentate Gyrus and CA1 in the hippocampus, basolateral amygdala and anterior cingulate cortex of mouse brain tissue.

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