scholarly journals High-Quality RNA from Cells Isolated by Laser Capture Microdissection

BioTechniques ◽  
2002 ◽  
Vol 33 (1) ◽  
pp. 176-179 ◽  
Author(s):  
A. Mikulowska-Mennis ◽  
T.B. Taylor ◽  
P. Vishnu ◽  
S.A. Michie ◽  
R. Raja ◽  
...  
Keyword(s):  
Laser Capture Microdissection ◽  
High Quality ◽  
Laser Capture

scholarly journals An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection

Plant Methods ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Muhammad Shahzad Anjam ◽  
Yvonne Ludwig ◽  
Frank Hochholdinger ◽  
Chisato Miyaura ◽  
Masaki Inada ◽  
...  
Keyword(s):  
Laser Capture Microdissection ◽  
High Quality ◽  
Laser Capture

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

10.3791/60197 ◽  
2019 ◽  
Author(s):  
Ana Marek ◽  
Christiane Schüler ◽  
María Satué ◽  
Barbara Haigl ◽  
Reinhold G. Erben
Keyword(s):  
Laser Capture Microdissection ◽  
High Quality ◽  
Fresh Frozen ◽  
Laser Capture

scholarly journals High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection

2009 ◽  
Vol 10 (1) ◽  
pp. 69 ◽  
Author(s):  
Wei-Zhi Wang ◽  
Franziska M Oeschger ◽  
Sheena Lee ◽  
Zoltán Molnár
Keyword(s):  
Laser Capture Microdissection ◽  
Brain Regions ◽  
High Quality ◽  
Laser Capture

scholarly journals Laser Capture Microdissection optimization for high-quality RNA in mouse brain tissue

2021 ◽  
Author(s):  
Margareth Nogueira ◽  
Daiane CF Golbert ◽  
Richardson Leão
Keyword(s):  
Brain Tissue ◽  
Mouse Brain ◽  
Laser Capture Microdissection ◽  
Basolateral Amygdala ◽  
Single Cells ◽  
Heterogeneous Material ◽  
Anterior Cingulate ◽  
High Quality ◽  
Rna Integrity ◽  
Laser Capture

Laser Capture Microdissection (LCM) is a method that allows to select and dissecting specific structures, cell populations, or even single cells from different types of tissue to extract DNA, RNA, or proteins. It is easy to perform and precise, avoiding unwanted signals from irrelevant cells, because gene expression may be affected by a bulk of heterogeneous material in a sample. However, despite its efficiency, several steps can affect the sample RNA integrity. In comparison to DNA, RNA is a much more unstable molecule and represents a challenge in the LCM method. Here we describe an optimized protocol to provide good concentration and high-quality RNA in specific structures, such as Dentate Gyrus and CA1 in the hippocampus, basolateral amygdala and anterior cingulate cortex of mouse brain tissue.

A Systematic Comparison of Protocols for Recovery of High-Quality RNA from Human Islets Extracted by Laser Capture Microdissection v1

2021 ◽  
Author(s):  
Chiara M. A. Cefalo ◽  
Teresa Mezza ◽  
Andrea Giaccari ◽  
Rohit N. Kulkarni
Keyword(s):  
Laser Capture Microdissection ◽  
Rna Extraction ◽  
Human Islets ◽  
Human Pancreas ◽  
Sequencing Analysis ◽  
High Quality ◽  
Partial Pancreatectomy ◽  
Rna Integrity ◽  
Laser Capture ◽  
Yield And Purity

The isolation of high-quality RNA from endocrine pancreas sections represents a considerable challenge largely due to the high ribonuclease levels. Laser capture microdissection (LCM) of mammalian islets, in association with RNA extraction protocols, has emerged as a feasible approach to characterizing their genetic and proteomic profiles. However, a validated protocol to obtain highquality RNA from LCM-derived human pancreas specimens that is appropriate for next-generation sequencing analysis is still lacking. In this study, we applied four methods (Picopure extraction kit, Qiazol protocol, Qiazol + Clean-up kit, and RNeasy Microkit + Carrier) to extract RNA from human islets obtained from both non-diabetic individuals and patients with type 2 diabetes who had undergone partial pancreatectomy, as well as handpicked islets from both non-diabetic and diabetic organ donors. The yield and purity of total RNA were determined by 260/280 absorbance using Nanodrop 100 and the RNA integrity number with a bioanalyzer. The results indicated that among the four methods, the RNeasy MicroKit + Carrier (Qiagen) provides the highest yield and purity.

scholarly journals Collection of Epithelial Cells from Rodent Mammary Gland Via Laser Capture Microdissection Yielding High-Quality RNA Suitable for Microarray Analysis

2010 ◽  
Vol 12 (1) ◽  
pp. 31-43 ◽  
Author(s):  
John N. McGinley ◽  
Zongjian Zhu ◽  
Weiqin Jiang ◽  
Henry J. Thompson
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Microarray Analysis ◽  
Laser Capture Microdissection ◽  
High Quality ◽  
Laser Capture
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