scholarly journals Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

10.3791/57762 ◽  
2018 ◽  
Author(s):  
Aaron A. May-Zhang ◽  
Karen K Deal ◽  
E. Michelle Southard-Smith
Keyword(s):  
Laser Capture Microdissection ◽  
Human Tissue ◽  
Enteric Ganglia ◽  
Fresh Frozen ◽  
Laser Capture

Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture Microdissection

2008 ◽  
pp. 35-45
Author(s):  
Dan-Arin Silasi ◽  
Ayesha B. Alvero ◽  
Jechiel Mor ◽  
Rui Chen ◽  
Han-Hsuan Fu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Laser Capture Microdissection ◽  
Fresh Frozen ◽  
Related Proteins ◽  
Laser Capture

Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture Microdissection

2008 ◽  
pp. 35-46
Author(s):  
Dan-Arin Silasi ◽  
Ayesha B. Alvero ◽  
Jechiel Mor ◽  
Rui Chen ◽  
Han-Hsuan Fu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Laser Capture Microdissection ◽  
Fresh Frozen ◽  
Related Proteins ◽  
Laser Capture

scholarly journals Tissue preparation for laser capture microdissection and RNA extraction from fresh frozen breast tissue

BioTechniques ◽  
2007 ◽  
Vol 43 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Mary Morrogh ◽  
Narciso Olvera ◽  
Faina Bogomolniy ◽  
Patrick I. Borgen ◽  
Tari A. King
Keyword(s):  
Laser Capture Microdissection ◽  
Breast Tissue ◽  
Rna Extraction ◽  
Tissue Preparation ◽  
Fresh Frozen ◽  
Laser Capture

scholarly journals Combined laser capture microdissection and serial analysis of gene expression from human tissue samples

2004 ◽  
Vol 18 (4) ◽  
pp. 577-584 ◽  
Author(s):  
Jeong Hee Cho-Vega ◽  
Patricia Troncoso ◽  
Kim-Anh Do ◽  
Carlo Rago ◽  
Xuemei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Laser Capture Microdissection ◽  
Human Tissue ◽  
Tissue Samples ◽  
Laser Capture

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

10.3791/60197 ◽  
2019 ◽  
Author(s):  
Ana Marek ◽  
Christiane Schüler ◽  
María Satué ◽  
Barbara Haigl ◽  
Reinhold G. Erben
Keyword(s):  
Laser Capture Microdissection ◽  
High Quality ◽  
Fresh Frozen ◽  
Laser Capture

scholarly journals A subcellular cookie cutter for spatial genomics in human tissue

2021 ◽  
Author(s):  
Alexander Bury ◽  
Angela Pyle ◽  
Fabio Marcuccio ◽  
Doug Turnbull ◽  
Amy Vincent ◽  
...  
Keyword(s):  
Laser Capture Microdissection ◽  
Human Tissue ◽  
The State ◽  
Precise Location ◽  
Cellular Physiology ◽  
First Time ◽  
Laser Capture ◽  
State Of Art ◽  
Biopsy Technology

Intracellular heterogeneity contributes significantly to cellular physiology and, in a number of debilitating diseases, cellular pathophysiology. This is greatly influenced by distinct organelle populations and to understand the aetiology of disease it is important to have tools able to isolate and differentially analyse organelles from precise location within tissues. Here we report the development of a subcellular biopsy technology that facilitates the isolation of organelles, such as mitochondria, from human tissue. We compared the subcellular biopsy technology to laser capture microdissection (LCM) that is the state of art technique for the isolation of cells from their surrounding tissues. We demonstrate an operational limit of (>20 micron) for LCM and then, for the first time in human tissue, show that subcellular biopsy can be used to isolate mitochondria beyond this limit.

Laser-Capture Microdissection for Layer-Specific Analysis of Enteric Ganglia

2018 ◽  
pp. 361-369
Author(s):  
Corinna Rosenbaum ◽  
Martina Böttner ◽  
Thilo Wedel ◽  
Marco Metzger
Keyword(s):  
Laser Capture Microdissection ◽  
Enteric Ganglia ◽  
Specific Analysis ◽  
Laser Capture

Isolating Human Notochord Using Laser Capture Microdissection

2016 ◽  
Vol 77 (S 02) ◽  
Author(s):  
Youssef Yakkioui ◽  
Remco Santegoeds ◽  
Koo van Overbeeke ◽  
Andreas Herrler ◽  
Yasin Temel
Keyword(s):  
Laser Capture Microdissection ◽  
Laser Capture
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