Increasing Durability of Dissociated Neural Cell Cultures Using Biologically Active Coralline Matrix

10.3791/60443 ◽  
2020 ◽  
Author(s):  
Orly Eva Weiss ◽  
Roni Mina Hendler ◽  
Danny Baranes
Keyword(s):  
Cell Cultures ◽  
Neural Cell ◽  
Biologically Active

Differentiation of primary embryonic neuroblasts in purified neural cell cultures from Drosophila

1985 ◽  
Vol 109 (1) ◽  
pp. 184-192 ◽  
Author(s):  
Allen Furst ◽  
Anthony P. Mahowald
Keyword(s):  
Cell Cultures ◽  
Neural Cell

Collagenase secretion accompanying changes in cell shape occurs only in the presence of a biologically active cytokine

1989 ◽  
Vol 92 (3) ◽  
pp. 473-485
Author(s):  
I. Kuter ◽  
B. Johnson-Wint ◽  
N. Beaupre ◽  
J. Gross
Keyword(s):  
Cell Cultures ◽  
Cell Shape ◽  
Cell Function ◽  
Shape Change ◽  
Biologically Active ◽  
Target Cells ◽  
Mixed Cell ◽  
Cell Shape Change ◽  
Cell Shape Changes ◽  
Shape Changes

We have investigated the relationship between collagenase production, cell shape and stimulatory factors in cell culture. In a homogeneous culture of primary rabbit corneal stromal cells, shape change induced by a variety of agents was not effective in stimulating collagenase secretion. Only in the presence of a biologically active cytokine or phorbol myristate acetate was a correlation seen between changes in cell shape (induced by a second agent) and collagenase secretion by these primary cells. Cell shape changes were not, however, necessary for collagenase secretion, since certain concentrations of endotoxin or lactalbumin hydrolysate effected secretion of the enzyme in the absence of morphological changes. With passaged cells or mixed cell cultures, where cell shape change did correlate with collagenase secretion without the addition of an exogenous agent, the production of an effective cytokine (autocrine or paracrine) was demonstrated. Thus cell shape change seems to be neither universally necessary nor sufficient for the stimulation of collagenase secretion. It is proposed that the function of cytokines may be more immediately related to gene expression in this system than is change in the shape of the cell. The hypothesis is presented that cell shape changes may render the target cells receptive to cytokines, perhaps by replacing the need for a natural cytokine cofactor. It is also demonstrated here that the use of passaged cells, mixed cell cultures containing endogenous cytokine-secreting cells or tissue culture additives can profoundly affect the interpretation of the effect of various agents on collagenase secretion, and may lead to observations that are not directly relevant to cell function in vivo.

Recent results toward the stereoselective synthesis of biologically active natural products

2007 ◽  
Vol 79 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Luiz C. Dias ◽  
Luciana G. de Oliveira ◽  
Paulo R. R. Meira
Keyword(s):  
Natural Products ◽  
Total Synthesis ◽  
Cell Cultures ◽  
Stereoselective Synthesis ◽  
Animal Cell ◽  
Biologically Active ◽  
Asymmetric Total Synthesis ◽  
Callystatin A ◽  
Animal Cell Cultures ◽  
Active Natural

This paper describes the convergent and stereocontrolled asymmetric total synthesis of (+)-crocacins C and D, potent inhibitors of animal cell cultures and several yeasts and fungi, and (-)-callystatin A, a potent antitumor polyketide.

Transport of L-Citrulline in Neural Cell Cultures

2000 ◽  
Vol 22 (5-6) ◽  
pp. 393-398 ◽  
Author(s):  
Andreas Schmidlin ◽  
Susanne Fischer ◽  
Heinrich Wiesinger
Keyword(s):  
Cell Cultures ◽  
Neural Cell

Structural and functional impairments of polysialic acid (polySia)-neural cell adhesion molecule (NCAM) synthesized by a mutated polysialyltransferase of a schizophrenic patient

2012 ◽  
Vol 84 (9) ◽  
pp. 1895-1906 ◽  
Author(s):  
Masaya Hane ◽  
Mizuki Sumida ◽  
Ken Kitajima ◽  
Chihiro Sato
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Polysialic Acid ◽  
Neural Cell ◽  
Biologically Active ◽  
Functional Impairments ◽  
Reservoir Function ◽  
Neural Cell Adhesion

Polysialic acid (polySia) is a homopolymer of sialic acid with a degree of polymerization (DP) of 8–400. When present on neural cell adhesion molecule (NCAM), polySia has anti-adhesive effects on cell–cell interactions owing to its bulky polyanionic nature, and is involved in the regulation of neurogenesis and neuronal functions. Recently, we demonstrated that polySia functions not only as an anti-cell adhesion molecule, but also as a reservoir scaffold for brain-derived neurotrophic factor (BDNF) and fibroblast growth factor 2 (FGF2), which are biologically active molecules in neurogenesis. To understand the significance of polySia structure in the reservoir function, we focused on polySia-NCAM biosynthesized by mutated polysialyltransferase (ST8SiaII or STX) that was reported in a schizophrenia patient. The polySia-NCAM biosynthesized by mutant ST8SiaII/STX contained less polySia with shorter chain length and exhibited impaired reservoir function for BDNF and FGF2 as compared with that synthesized by wild-type (wt) ST8SiaII/STX. Our findings suggest that the quantity and quality of polySia on NCAM are important for normal neuronal functioning.

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