Biologically Active Substances from Powdered Barberry and Viburnum

Introduction. Plant raw materials can be a source of biologically active substances and increase the nutritional value of food products. The present research objective was to determine the content of biologically active substances in powdered viburnum and barberry. Study objects and methods. The study featured viburnum (Viburnum opulus L.) and barberry (Berberis vulgaris L.), dried by convection and crushed into particles of 50 microns. Results and discussion. The total content of phenolic compounds in powdered viburnum was 3114.07 mg/100 g, in powdered barberry – 2272.7 mg/100 g. The content of flavonoids in powdered viburnum was 324.52 mg/100 g, in powdered barberry – 390.00 mg/100 g. The flavonoid profile of the powders included rutin, hyperoside, quercitrin, isoquercintrin, and astralagin. The total content of catechins was 446 mg/100 g for viburnum and 506 mg/100 g for barberry. The catechins included mainly epigallocatechin and catechin. In powdered viburnum, the catechin composition was as follows: epicatechin – 196, catechin – 118, and epigallocatechin – 89 mg/100 g; in powdered barberry: epigallocatechin – 173, catechin – 111, and epicatechin – 74 mg/100 g. The antiradical activity in relation to trolox equivalent was 7560 mg/100 g in powdered viburnum and 9460 mg/100 g in powdered barberry. Conclusion. The obtained viburnum and barberry powders can fortify food with biologically active substances and expand the range of functional products.

Modern Technologies for Producing Foamed Phosphate Glass for Oil Sorbents

The paper considers applications of foamed glassy phosphate materials as carriers of biologically active substances. The atomic layer deposition method allowed chemically synthesizing surface-modified composite materials based on graphite and carbon fibers, which maximally preserved the activity of enzymes and biologically active substances. The synthesis process of a mono-layer titanium-containing coating on the surface of graphite and carbon fibers by processing them with TiCl4 vapors is considered. The main characteristics of the graphite surface before and after modification and characteristics of the porous structure and surface of carbon fibers are obtained. Biologically active substances by adsorption from a solution to the surface of both the original and modified carriers were applied. biological activity and temperature stability of the obtained composite materials were studied. It is shown that the activity of a biologically active substance depends on the chemical composition and state of the surface of carbon-based carriers.

The Influence of the Parameters of the Subcritical Water Extraction Process on the Efficiency of Isoflavones Elicitation from Soy Meal

Topicality. Incidental products of soy processing (okara, meal, millcake) contain a large amount of protein, and, additionally, other valuable biologically active substances. Among them, isoflavones are of particular researchers’ interest. Subcritical water extraction is a promising method for elicitation biologically active substances from plant raw material. However, such a method of extracting isoflavones, and its rational parameters for soy meal, remain insufficiently investigated. Aim and methods. The aim of this work is to study the influence of parameters (temperature: 120...160°С, duration of extraction: 5...15 min, hydromodule: 1:15...25) of the extracting process of soy meal with subcritical water on the isoflavones content in the dry material of the extract. The empirical research method is applied, using the second-order orthogonal compositional outline for a fractional factor experiment. The samples of soy meal extract are obtained using the experimental setup, based on the high-pressure reactor “RHP-2-500”. The total isoflavones content in the dry extract material is defined in terms of gallic acid with absorption spectrophotometry method. Results. Based on the obtained results, the quadratic interpolation model is constructed. This model links the total isoflavones content in the dry extract material with the extraction parameters. The analysis of the constructed model makes it possible to establish rational measures of extraction parameters. Conclusions and discussion. In this study, the intensive growth of soflavones content in the dry extract with highing the temperature process, and a relatively small influence of the process duration on this indicator, were defined. The influence of the hydromodule is ambiguous. The lowest yield of isoflavones is noted at hydromodule ≈1:21,41. Its increase or decrease lead to the growth of isoflavones content in the extract. In this paper, for the first time, the existence of rational measures of parameters in the process of extracting isoflavones with subcritical water from soy meal is studied and established. The obtained results can be used in the elaboration of the technology for producing isoflavones enriched soy protein concentrate.

CONSUMER PROPERTIES OF PASTA WITH POWDER FROM MARIGOLD INFLORESCENCES (TAGETES L.)

Background. The deficiency of essential nutrients in the human body is the root cause of reduced efficiency, general resistance of the body to various diseases. That is an urgent problem in the context of the spread of coronavirus infection. In this regard, the search for opportunities to meet the needs of the population in food products that contain biologically active substances (BAS) through the use of high-value and at the same time available plant raw materials is extremely important. Marigolds attract special attention among such raw materials. The aim of the work is to study the chemical composition and evaluate the consumer properties of Tagetes L. varieties, zoned in the Kiev region, to prove the possibility of increasing the biological value of pasta by adding to their recipe powder from marigold inflorescences. Materials and methods. The object of research is pasta of B group (noodles). Inflorescences of marigolds of different varieties gathered during the flowering period in July-September 2019–2020 in the territory of Vasylkiv and Obukhiv districts of Kyiv region, Ukraine were used as enriching plant raw materials. The quality of finished pasta was assessed by organoleptic and physicochemical indexes (moisture content and titratable acidity). The content of mineral elements in the fine powder of marigold inflorescences was determined on the EXHERT-3L device, the quantitative composition and BAS identification – by spectrophotometric analysis on the device Specord-200 Analytic Jena UV-vis. The integrated quality index of pasta products was calculated taking into account the importance factors of individual indexes. Results. Comparing the obtained results, we can state that all the studied varieties – Gold Kopfen, Orange Flame, Hawaii, Equinox are characterized by high biological value, regardless of the area of collection. As a result of calculated and experimental studies it was determined that the addition of 7 % of the supplement does not provide the required supply of vitamins and minerals. Replacing the flour in the recipe by 20 % significantly improves the mineral composition, but deteriorates the taste and aromatic properties of pasta. The most optimal and rational amount of added additive is 15 %, as it improves the organoleptic properties of pasta and enriches their BAS. Conclusion. The usage of non-traditional vegetable raw materials, in particular powder from marigold inflorescences, which is rich in biologically active substances, allows not only to expand the range, improve the quality of finished products, but also increase the biological value of the product. The optimal amount of additive –powder from marigold inflorescences, was defined. It is 15 % by weight of flour in the production of noodles. This concentration allows not only to improve the organoleptic properties, but also to increase the content of biologically active substances – vitamins, micro- and macronutrients. The usage of non-traditional natural raw materials (powder from marigold inflorescences) allows to abandon artificial colorsand give the finished pasta a pleasant colorand aroma.

JELLY FOOD CONCENTRATE WITH ENCAPSULATED HIBISCUS EXTRACT

Background. Taking into consideration the intense rhythm of life, the development of technologies for instant food products of increased nutritional value is urgent. Typically, food concentrates for desserts include fruit or fruit and berry fillings and require cooking to prepare ready-made meals. In this case, heat treatment of the mixture (boiling) negatively affects the thermolabile biologically active compounds. The aim of the work was to develop a recipe for instant food jelly concentrates of increased nutritional value based on encapsulated powdery hibiscus extract. Materials and methods. To encapsulate the aqueous-alcoholic extract of hibiscus and obtain a powdered semi-finished product, swelling potato starch was used. Rheological studies were carried out using a "REOTEST-2" rotational viscometer. Results. In order to enrich food concentrates of instant kissel with biologically active substances, we studied a method for extracting dry hibiscus flowers with aqueous-alcoholic solutions of various concentrations. It was found that the extractivity and the transfer of polyphenolic compounds, including anthocyanins, to the extract are improved when a water-alcohol mixture with an ethyl alcohol concentration of 30 % is used as an extractant. For the preparation of a powdery hibiscus extract, modified swelling starch was used, which has the property of swelling in cold water, which makes it possible not to subject the mixture to prolonged heat treatment for preparing a ready-made dish and to preserve the biologically active substances of the hibiscus extract. In addition, this starch is an effective solid carrier that plays a protective role for thermolabile vitamins and bioflavonoids. On the basis of experimental studies, formulations of instant jelly have been developed, which include fructose, powdered hibiscus extract, apple pectin and ascorbic acid. The study of the rheological properties of semi-finished products based on hibiscus extract, as well as ready-made jelly, testifies to the receipt of a finished dessert with a delicate creamy consistency. Conclusion. A method for obtaining a powdery encapsulated hibiscus extract based on swelling potato starch and a composition of a food jelly concentrate using this semi-finished product have been developed. It was found that for the extraction of raw materials it is better to use a water-alcohol mixture with an ethanol content of 30 %, since most of the anthocyanins are converted into the extract under these conditions. The developed semi-finished product of dry hibiscus extract can be used as a thickener, acidifier and colorant in recipes for food concentrates for instant dessert dishes. Due to the use of swelling starch, food concentrates of jelly do not need to be boiled, and the presence of vitamins, organic acids, flavonoids and especially anthocyanins in the hibiscus extract increases the nutritional value of the finished product.

PROSPECTS FOR THE DEVELOPMENT OF FEED PRODUCTION FOR FUR ANIMALS

The article considers the species of fur animals bred in Ukraine. The volumes of fur production and the number of animal farms in the world and Ukraine are analyzed, of which Denmark, China and Poland are in the top three. The share in the total volume of fur production in Ukraine is only 1%. Domestic enterprises for breeding fur animals, which keep about 700 thousand minks in industrial conditions, are considered.The structure of the gastrointestinal tract of carnivorous and herbivorous fur animals, their consumption of nutrients and biologically active substances in feed were analyzed. Features of feeding fur animals are determined by the seasonality of vital functions, which determines changes in metabolism and nutritional value of feed. The importance of access to fresh drinking water in the diets of carnivorous fur animals is considered.The necessity of production of compound feeds for fur animals, which meet the norms of feeding and restrictions on the introduction of components, is substantiated.The need of fur-bearing animals in nutrients and biologically active substances for the production of high-quality nutritious compound feeds, for obtaining high-quality shine and density of fur, better digestibility by the body is shown. The ratios of digestible nutrients in the diets of minks of domestic and Scandinavian breeds are given, the percentage of metabolic energy in different feeding periods is given. The low percentage of compound feed production for fur farming is substantiated.Types of compound feeds and their advantages are presented. Technological methods of production of compound feeds for fur animals such as extrusion, expansion and granulation with the introduction of fat into the press granulator are analyzed. Finished feed should be solid and have a granule size of 4.5… 5 mm for grinding cutters, with high nutritional properties and biological value, and improved sanitation. Thanks to modern technologies, harmful microflora is disinfected and shelf life is increased.The state of domestic feed mills for the production of quality feed for fur animals is analyzed. Thanks to the emergence of new recipes and compliance with feeding standards, the technology of compound feed production for fur farming is being improved.

Chemical Composition and Content of Biologically Active Substances Found in Cotinus coggygria, Dactylorhiza maculata, Platanthera chlorantha Growing in Various Territories

Medicinal plants (Cotinus coggygria, Dactylorhiza maculata, Platanthera chlorantha) growing in various territories (Kaliningrad, Moscow, and Minsk regions) were the objects of research. This paper presents a study of the chemical composition of these plants. To analyze the qualitative and quantitative composition of biologically active substances, the method of high-performance liquid chromatography was used. Atomic absorption spectrometry was used to study the content of trace elements. The content of organic acids and vitamins was determined by capillary electrophoresis using the Kapel-105/105M capillary electrophoresis system with high negative polarity. Extracts of medicinal plants were obtained on a Soxhlet apparatus using 70% ethanol as an extractant. It was found that among the biologically active substances in the plants under discussion, hyperoside, rutin (C. coggygria), Ferulic acid and Gallic acid (D. maculata), triene hydrocarbon (3,7-Dimethyl-1,3,6-octatriene), unsaturated alcohol (3,7-Dimethyl-2,6-octadien-1-ol), and benzyl acetate (P. chlorantha) prevailed. Samples of these medicinal plants contained trace elements (phosphorus, potassium, calcium, sodium, magnesium, and sulfur) and many aliphatic organic acids (succinic acid, benzoic acid, fumaric acid, citric acid, oxalic acid, and tartaric acid). The largest amount of biologically active substances and secondary metabolites of the studied plants from the Eastern Baltic is associated with climatic and ecological differences from other regions. The composition of these plants determines the potential of their use in feed additives for livestock and poultry as part of measures to improve the quality of livestock products. The use of medicinal plants for the production of feed additives is relevant in terms of improving regional economies, as well as improving the quality of life and nation’s health by providing ecologically clean livestock products.

QUALITATIVE AND QUANTITATIVE ANALYSIS OF FLAVONOIDS DURING DEVELOPMENT OF ANTIDIABETIC EXTRACT

During development of antidiabetic extract in blueberry shoots (Vaccinium myrtillus L.), ginseng leaves (Panax ginseng C.A.Mey.) and water-alcohol extraction from this plant material the flavonoid content is studied because this group of biologically active substances has hypoglycemic effect. Qualitative reactions for different groups of flavonoids has confirmed their presence in blueberry shoots and ginseng leaves. In addition, qualitative analysis of water-alcohol extraction on their base using spectrophotometry has also showed presence of this group of biologically active substances. Along with that, quantitative content of flavonoids has been detected using differential spectrophotometry in blueberry shoots, ginseng leaves and water-alcohol extraction on their base.