scholarly journals Induction of Biologically Active Flavonoids in Cell Cultures of Morus nigra and Testing their Hypoglycemic Efficacy

2011 ◽  
Vol 79 (4) ◽  
pp. 951-961 ◽  
Author(s):  
Ahmed M. A. Abd El-Mawla
Keyword(s):  
Cell Cultures ◽  
Biologically Active ◽  
Morus Nigra

Collagenase secretion accompanying changes in cell shape occurs only in the presence of a biologically active cytokine

1989 ◽  
Vol 92 (3) ◽  
pp. 473-485
Author(s):  
I. Kuter ◽  
B. Johnson-Wint ◽  
N. Beaupre ◽  
J. Gross
Keyword(s):  
Cell Cultures ◽  
Cell Shape ◽  
Cell Function ◽  
Shape Change ◽  
Biologically Active ◽  
Target Cells ◽  
Mixed Cell ◽  
Cell Shape Change ◽  
Cell Shape Changes ◽  
Shape Changes

We have investigated the relationship between collagenase production, cell shape and stimulatory factors in cell culture. In a homogeneous culture of primary rabbit corneal stromal cells, shape change induced by a variety of agents was not effective in stimulating collagenase secretion. Only in the presence of a biologically active cytokine or phorbol myristate acetate was a correlation seen between changes in cell shape (induced by a second agent) and collagenase secretion by these primary cells. Cell shape changes were not, however, necessary for collagenase secretion, since certain concentrations of endotoxin or lactalbumin hydrolysate effected secretion of the enzyme in the absence of morphological changes. With passaged cells or mixed cell cultures, where cell shape change did correlate with collagenase secretion without the addition of an exogenous agent, the production of an effective cytokine (autocrine or paracrine) was demonstrated. Thus cell shape change seems to be neither universally necessary nor sufficient for the stimulation of collagenase secretion. It is proposed that the function of cytokines may be more immediately related to gene expression in this system than is change in the shape of the cell. The hypothesis is presented that cell shape changes may render the target cells receptive to cytokines, perhaps by replacing the need for a natural cytokine cofactor. It is also demonstrated here that the use of passaged cells, mixed cell cultures containing endogenous cytokine-secreting cells or tissue culture additives can profoundly affect the interpretation of the effect of various agents on collagenase secretion, and may lead to observations that are not directly relevant to cell function in vivo.

Recent results toward the stereoselective synthesis of biologically active natural products

2007 ◽  
Vol 79 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Luiz C. Dias ◽  
Luciana G. de Oliveira ◽  
Paulo R. R. Meira
Keyword(s):  
Natural Products ◽  
Total Synthesis ◽  
Cell Cultures ◽  
Stereoselective Synthesis ◽  
Animal Cell ◽  
Biologically Active ◽  
Asymmetric Total Synthesis ◽  
Callystatin A ◽  
Animal Cell Cultures ◽  
Active Natural

This paper describes the convergent and stereocontrolled asymmetric total synthesis of (+)-crocacins C and D, potent inhibitors of animal cell cultures and several yeasts and fungi, and (-)-callystatin A, a potent antitumor polyketide.

scholarly journals The fatty acids content of calli cultures from different explant types of amarantus plants

1970 ◽  
pp. 184-189
Author(s):  
V. P. Zhuk ◽  
L. O. Sakhno ◽  
M. A. Kharkhota ◽  
S. V. Isaienkov
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
In Vitro Culture ◽  
Acid Composition ◽  
Cell Cultures ◽  
Biologically Active ◽  
Leaf Discs ◽  
Different Types ◽  
Calli Cultures

Aim. The aim of our study was selection of optimal conditions for the initiation of Amaranthus L. aseptic callus in vitro culture from different types of explants and estimation of fatty acid composition in these types of cell cultures. Methods. In vitro culture using leaf disks and internodes as explants. Inert gas chromatography. Results. The optimized Gamborg medium for callus induction was designed (sucrose 25 g/1L; 2.4-D 0.5 mg/L; NAA 1 mg/L). The kinetin in concentration 0.5 mg/L for internodes and for leaf discs explants were added. The fatty acid profiles of calli cultures from the different types of plant explants were analyzed The highest level of Omega-3 fatty acid were detected in cell cultures from internodes and leaf discs of cultivar Helios. Conclusions. Our optimized protocol for Amaranthus callus initiation could be used for further studies of the synthesis and accumulation of biologically active metabolites in Amaranthus tissue culture. The fatty acid composition of calli cultures depend from explant type as well as from plant cultivar. Keywords: Amaranthus, callus, in vitro culture, fatty acid composition

scholarly journals Growing optimization of suspension cultures of medicinal plant cells

2021 ◽  
Vol 291 ◽  
pp. 02022
Author(s):  
Andrey Kolomietc ◽  
Nadezda Nicolaeva ◽  
Victoria Larina ◽  
Nataliya Chupakhina
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Cell Cultures ◽  
Plant Material ◽  
Plant Cells ◽  
Biologically Active ◽  
Suspension Cell ◽  
Active Metabolites ◽  
Isolated Cells

Suspension cell cultures allow to save plant material when obtaining biologically active compounds of natural origin. As a result of the studies, optimal parameters were selected to increase the formation of biologically active metabolites in suspension cell cultures of such medicinal plants as Maackia amurensis Rupr., Hyssopus officinalis L. and Saposhnikovia divaricata (Turcz.) Schischk. Medicinal plants are a large group of plants used as raw materials for the production of medicinal and preventive drugs for medical and animal use. The assortment of phytopreparations is constantly expanding due to the increased demand for natural remedies, due to their less aggressive and toxic nature compared to synthetic ones [1]. Cultivation of medicinal plants in the form of isolated cells in vitro is one of the most modern technologies for rapidly obtaining a large biomass of plant material with stable growth features year-round under controlled conditions [2]. It is known that cells in vitro grow faster and have peculiarities of synthesis and accumulation of biologically active substances compared to intact plants [3]. Isolated cells, unlike tissue cells, also have an advantage for their use as a source of active metabolites, since they have the ability to release these compounds into the intercellular space [4]. The goal of this paper was to select parameters for increasing the biosynthetic activity of cultured suspension cultures of medicinal plant cells in vitro by optimizing cultivation conditions and introducing precursors of secondary metabolite biosynthesis into the nutrient media.

Growth Inhibitions on Human Cancer Cell Cultures with the Indole Sulphur-Containing Phytoalexins and Their Analogues

1991 ◽  
Vol 46 (7-8) ◽  
pp. 706-708 ◽  
Author(s):  
Christiane Tempête ◽  
Michel Devys ◽  
Michel Barbier
Keyword(s):  
Cell Growth ◽  
Cancer Cell ◽  
Cell Cultures ◽  
Human Cancer ◽  
Biologically Active ◽  
Human Cancer Cell ◽  
Synthetic Analogues ◽  
Cruciferae Family

Abstract Cell growth inhibitions on human cancer cell cultures were determined for the indole sulphur-containing phytoalexins cyclobrassinin, brassilexin (previously isolated from vegetables of the Cruciferae family) and their synthetic analogues 5-methoxybrassilexin and homocyclobrassinin. The most biologically active of these products is brassilexin (LD50= 8 μg/ml).

Strategies to enhance biologically active-secondary metabolites in cell cultures ofArtemisia– current trends

2017 ◽  
Vol 37 (7) ◽  
pp. 833-851 ◽  
Author(s):  
Mohammad Ali ◽  
Bilal Haider Abbasi ◽  
Nisar Ahmad ◽  
Haji Khan ◽  
Gul Shad Ali
Keyword(s):  
Secondary Metabolites ◽  
Cell Cultures ◽  
Biologically Active ◽  
Current Trends

The longevity of biologically-active oligogalacturonides in rose cell cultures: degradation by exo-polygalacturonase

1995 ◽  
Vol 46 (12) ◽  
pp. 1853-1857 ◽  
Author(s):  
Inmaculada García-Romera ◽  
Stephen C. Fry
Keyword(s):  
Cell Cultures ◽  
Biologically Active
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