Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

10.3791/61956 ◽  
2021 ◽  
Author(s):  
Changhua Zhu ◽  
Xiaoye Liu ◽  
Wenlei Wang ◽  
Xiaoguang Chen ◽  
Shangyu Gao ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Plant Sample ◽  
Nucleotide Content ◽  
Content Measurement

scholarly journals Rapid immunohistochemical diagnosis of tobacco mosaic virus disease by microwave-assisted plant sample preparation

Microscopy ◽  
2013 ◽  
Vol 62 (5) ◽  
pp. 547-553 ◽  
Author(s):  
Günther Zellnig ◽  
Stefan Möstl ◽  
Bernd Zechmann
Keyword(s):  
Sample Preparation ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Disease ◽  
Plant Sample ◽  
Microwave Assisted ◽  
Immunohistochemical Diagnosis

Oolong tea and LR-White resin: a new method of plant sample preparation for transmission electron microscopy

2018 ◽  
Vol 270 (2) ◽  
pp. 244-251 ◽  
Author(s):  
XIAOHUA HE ◽  
FUZHEN GUO ◽  
BIN LIU
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sample Preparation ◽  
New Method ◽  
Plant Sample ◽  
Oolong Tea ◽  
Lr White ◽  
Transmission Electron

scholarly journals Effect of plant sample preparation and measuring methods on ATR-FTIR spectra results

2020 ◽  
Vol 169 ◽  
pp. 103915 ◽  
Author(s):  
Tomasz Durak ◽  
Joanna Depciuch
Keyword(s):  
Sample Preparation ◽  
Ftir Spectra ◽  
Plant Sample ◽  
Measuring Methods

Detection and identification of bacterial canker of tomato Clavibacter michiganensis subsp. michiganensis

2021 ◽  
Author(s):  
I.N. Pisareva ◽  
◽  
O.Yu. Slovareva ◽  
Keyword(s):  
Sample Preparation ◽  
Plant Material ◽  
Plant Sample ◽  
Bacterial Canker ◽  
Clavibacter Michiganensis ◽  
Diagnostic Protocol ◽  
Clavibacter Michiganensis Subsp ◽  
Detection And Identification ◽  
Bacterial Canker Of Tomato

The study is devoted to the diagnosis of bacterial canker of tomato (Cmm). The method of sampling and plant sample preparation has been adapted. PCR recommended by the international diagnostic protocol and other sources have been tested. The use of methods made it possible to identify Cmm in plant material

scholarly journals SP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS

2021 ◽  
Vol 12 ◽  
Author(s):  
Kamil Mikulášek ◽  
Hana Konečná ◽  
David Potěšil ◽  
Renata Holánková ◽  
Jan Havliš ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Magnetic Particles ◽  
Protein Extraction ◽  
Solid Phase ◽  
Handling Time ◽  
Plant Sample ◽  
Preparation Methods ◽  
Wide Range ◽  
Contaminants Removal ◽  
Paramagnetic Beads

Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. Four sample preparation methods, including the filter-aided sample preparation (FASP), two single-pot solid-phase-enhanced sample preparations (SP3) on carboxylated or HILIC paramagnetic beads, and protein suspension trapping method (S-Trap) were evaluated for SDS removal and protein digestion from Arabidopsis thaliana (AT) lysate. Finally, the optimized carboxylated SP3 workflow was benchmarked closely against the routine FASP. Ultimately, LC-MS/MS analyses revealed that regarding the number of identifications, number of missed cleavages, proteome coverage, repeatability, reduction of handling time, and cost per assay, the SP3 on carboxylated magnetic particles proved to be the best alternative for SDS and other contaminants removal from plant sample lysate. A robust and efficient 2-h SP3 protocol for a wide range of protein input is presented, benefiting from no need to adjust the amount of beads, binding and rinsing conditions, or digestion parameters.

Chlorogenic Acid Stability in Pressurized Liquid Extraction Conditions

2015 ◽  
Vol 98 (2) ◽  
pp. 415-421 ◽  
Author(s):  
Dorota Wianowska ◽  
Rafał Typek ◽  
Andrzej L Dawidowicz
Keyword(s):  
Sample Preparation ◽  
Higher Plants ◽  
Food Additives ◽  
Transformation Process ◽  
Liquid Extraction ◽  
Chemical Degradation ◽  
Pressurized Liquid Extraction ◽  
Plant Sample ◽  
Natural Occurrence ◽  
Reaction With Water

Abstract Chlorogenic acids (CQAs) are phenolic compounds naturally occurring in all higher plants. They are potentially useful in pharmaceuticals, foodstuffs, food additives, and cosmetics due to their recently suggested biomedical activity. Hence, research interestin CQA properties, their isomers, and natural occurrence has been growing. Pressurized liquid extraction(PLE) is regarded as an effective and quick sample preparation method in plant analysis. The short time of PLE decreases the risk of chemical degradation ofextracted compounds, thus increasing the attractiveness of its application. However, PLE applied for plant sample preparation is not free from limitations. We found that trans-5-O-caffeoylquinic acid (trans-5-CQA), the main CQA isomer, isomerizes to 3- and 4-O-caffeoylquinic acids and undergoes transesterification, hydrolysis, and reaction with water even in rapid PLE. Moreover, the number and concentration of trans-5-CQA derivatives formed in PLE strongly depends on extractant composition, its pH, and extraction time and temperature. It was not possible to find the PLE conditions in which the transformation process of trans-5-CQA would be eliminated.

Essential Methods of Plant Sample Preparation for Light Microscopy

2019 ◽  
pp. 1-26 ◽  
Author(s):  
Aleš Soukup ◽  
Edita Tylová
Keyword(s):  
Sample Preparation ◽  
Light Microscopy ◽  
Plant Sample

A study of plant sample preparation and inductively coupled plasma emission spectrometry parameters

1996 ◽  
Vol 27 (5-8) ◽  
pp. 1177-1198 ◽  
Author(s):  
B. Kovács ◽  
Z. Gyôri ◽  
J. Prokisch ◽  
J. Loch ◽  
P. Dániel
Keyword(s):  
Sample Preparation ◽  
Inductively Coupled Plasma ◽  
Emission Spectrometry ◽  
Plant Sample ◽  
Plasma Emission ◽  
Inductively Coupled ◽  
Plasma Emission Spectrometry
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