Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform

10.3791/62539 ◽  
2021 ◽  
Author(s):  
Jaimeson Veldhuizen ◽  
Mehdi Nikkhah
Keyword(s):  
Cardiac Tissue ◽  
Microfluidic Platform ◽  
Human Cardiac Tissue

MZF1 is differentially expressed in human cardiac tissue of different age and c-KIT+ progenitor cells in the human adult heart

2014 ◽  
Vol 62 (S 01) ◽  
Author(s):  
S. Doppler ◽  
M. Dreßen ◽  
H. Lahm ◽  
A. Werner ◽  
M.-A. Deutsch ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cardiac Tissue ◽  
Differentially Expressed ◽  
Adult Heart ◽  
Human Adult ◽  
Human Cardiac Tissue

The Nonselective β-Blocker Carvedilol Suppresses Apoptosis in Human Cardiac Tissue: A Pilot Study

2010 ◽  
Vol 13 (4) ◽  
pp. E218-E222 ◽  
Author(s):  
Engin Usta ◽  
Migdat Mustafi ◽  
Andreas Straub ◽  
Gerhard Ziemer
Keyword(s):  
Pilot Study ◽  
Cardiac Tissue ◽  
Β Blocker ◽  
Human Cardiac Tissue

scholarly journals Cardiac Organoids to Model and Heal Heart Failure and Cardiomyopathies

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 563
Author(s):  
Magali Seguret ◽  
Eva Vermersch ◽  
Charlène Jouve ◽  
Jean-Sébastien Hulot
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Cardiac Cells ◽  
Cardiac Functions ◽  
Different Types ◽  
Recent Developments ◽  
Human Cardiac Tissue ◽  
Key Aspects

Cardiac tissue engineering aims at creating contractile structures that can optimally reproduce the features of human cardiac tissue. These constructs are becoming valuable tools to model some of the cardiac functions, to set preclinical platforms for drug testing, or to alternatively be used as therapies for cardiac repair approaches. Most of the recent developments in cardiac tissue engineering have been made possible by important advances regarding the efficient generation of cardiac cells from pluripotent stem cells and the use of novel biomaterials and microfabrication methods. Different combinations of cells, biomaterials, scaffolds, and geometries are however possible, which results in different types of structures with gradual complexities and abilities to mimic the native cardiac tissue. Here, we intend to cover key aspects of tissue engineering applied to cardiology and the consequent development of cardiac organoids. This review presents various facets of the construction of human cardiac 3D constructs, from the choice of the components to their patterning, the final geometry of generated tissues, and the subsequent readouts and applications to model and treat cardiac diseases.

Abstract 020: Cell-sheet-based Bioengineered Human Cardiac Tissue Using Pluripotent Stem Cells For Heart Repair And Disease Models

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Katsuhisa Matsuura ◽  
Tatsuya Shimizu ◽  
Nobuhisa Hagiwara ◽  
Teruo Okano
Keyword(s):  
Cardiac Tissue ◽  
Subcutaneous Tissue ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Embryoid Bodies ◽  
Cardiac Differentiation ◽  
Cardiac Cell ◽  
Cell Sheets ◽  
High Efficient ◽  
Human Cardiac Tissue

We have developed an original scaffold-free tissue engineering approach, “cell sheet engineering”, and this technology has been already applied to regenerative medicine of various organs including heart. As the bioengineered three-dimensional cardiac tissue is expected to not only function for repairing the broad injured heart but also to be the practicable heart tissue models, we have developed the cell sheet-based perfusable bioengineered three-dimensional cardiac tissue. Recently we have also developed the unique suspension cultivation system for the high-efficient cardiac differentiation of human iPS cells. Fourteen-day culture with the serial treatments of suitable growth factors and a small compound in this stirring system with the suitable dissolved oxygen concentration produced robust embryoid bodies that showed the spontaneous beating and were mainly composed of cardiomyocytes (~80%). When these differentiated cells were cultured on temperature-responsive culture dishes after the enzymatic dissociation, the spontaneous and synchronous beating was observed accompanied with the intracellular calcium influx all over the area even after cell were detached from culture dishes as cell sheets by lowering the culture temperature. The cardiac cell sheets were mainly composed of cardiomyocytes (~80%) and partially mural cells (~20%). Furthermore, extracellular action potential propagation was observed between cell sheets when two cardiac cell sheets were partially overlaid, and this propagation was inhibited by the treatment with some anti-arrhythmic drugs. When the triple layered cardiac tissue was transplanted onto the subcutaneous tissue of nude rats, the spontaneous pulsation was observed over 2 months and engrafted cardiomyocytes were vascularized with the host tissue-derived endothelial cells. These findings suggest that cardiac cell sheets formed by hiPSC-derived cardiomyocytes might have sufficient properties for the creation of thickened cardiac tissue. Now we are developing the vascularized thickened human cardiac tissue by the repeated layering of cardiac cell sheets on the artificial vascular bed in vitro.

Abstract 366: Engineered Human Cardiac Tissue from Skeletal Muscle Derived Cells and Induced Pluripotent Stem Cell Derived Cardiomyocytes

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Kimimasa Tobita ◽  
Jason S Tchao ◽  
Jong Kim ◽  
Bo Lin ◽  
Johnny Huard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cardiac Tissue ◽  
Troponin I ◽  
Troponin T ◽  
Induced Pluripotent Stem Cell ◽  
Human Cardiac Tissue ◽  
Induced Pluripotent ◽  
Immature Myocardium

We have previously shown that rat skeletal muscle derived stem cells differentiate into an immature cardiomyocyte (CM) phenotype within a 3-dimensional collagen gel engineered cardiac tissue (ECT). Here, we investigated whether human skeletal muscle derived progenitor cells (skMDCs) can differentiate into a CM phenotype within ECT similar to rat skeletal muscle stem cells and compared the human skMDC-ECT properties with ECT from human induced pluripotent stem cell (iPSc) derived CMs. SkMDCs differentiated into a cardiac muscle phenotype within ECT and exhibited spontaneous beating activity as early as culture day 4 and maintained their activity for more than 2 weeks. SkMDC-ECTs stained positive for cardiac specific troponin-T and troponin-I, and were co-localized with fast skeletal muscle myosin heavy chain (sk-fMHC) with a striated muscle pattern similar to fetal myocardium. The iPS-CM-ECTs maintained spontaneous beating activity for more than 2 weeks from ECT construction. iPS-CM stained positive for both cardiac troponin-T and troponin-I, and were also co-localized with sk-fMHC while the striated expression pattern of sk-fMHC was lost similar to post-natal immature myocardium. Connexin-43 protein was expressed in both engineered tissue types, and the expression pattern was similar to immature myocardium. The skMDC-ECT significantly upregulated expression of cardiac-specific genes compared to conventional 2D culture. SkMDC-ECT displayed cardiac muscle like intracellular calcium ion transients. The contractile force measurements demonstrated functional properties of fetal type myocardium in both ECTs. Our results suggest that engineered human cardiac tissue from skeletal muscle progenitor cells mimics developing fetal myocardium while the engineered cardiac tissue from inducible pluripotent stem cell-derived cardiomyocytes mimics post-natal immature myocardium.

Shortcomings of Animal Models and the Rise of Engineered Human Cardiac Tissue

2017 ◽  
Vol 3 (9) ◽  
pp. 1884-1897 ◽  
Author(s):  
Barry Fine ◽  
Gordana Vunjak-Novakovic
Keyword(s):  
Animal Models ◽  
Cardiac Tissue ◽  
Human Cardiac Tissue

scholarly journals Microbiological examination of donated human cardiac tissue in heart valve banking

2010 ◽  
Vol 37 (1) ◽  
pp. 163-169 ◽  
Author(s):  
Jorge P. van Kats ◽  
Corina van Tricht ◽  
Alice van Dijk ◽  
Michel van der Schans ◽  
Antoon van den Bogaerdt ◽  
...  
Keyword(s):  
Heart Valve ◽  
Cardiac Tissue ◽  
Microbiological Examination ◽  
Human Cardiac Tissue

Nonexocytotic noradrenaline release induced by pharmacological agents or anoxia in human cardiac tissue

1996 ◽  
Vol 354 (1) ◽  
Author(s):  
Thomas Kurz ◽  
Gert Richardt ◽  
Melchior Seyfarth ◽  
Albert Sch�mig
Keyword(s):  
Noradrenaline Release ◽  
Cardiac Tissue ◽  
Pharmacological Agents ◽  
Human Cardiac Tissue
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