Process Development for the Production and Purification of Adeno-Associated Virus (AAV)2 Vector using Baculovirus-Insect Cell Culture System

10.3791/62829 ◽  
2022 ◽  
Author(s):  
Md Nasimuzzaman ◽  
Sophia Villaveces ◽  
Johannes C. M. van der Loo ◽  
Sivani Alla
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture System ◽  
Process Development ◽  
Insect Cell Culture ◽  
Cell Culture System ◽  
Adeno Associated Virus

Multiplication and differentiation of Trypanosoma cruzi in an insect cell culture system

1969 ◽  
Vol 24 (2) ◽  
pp. 176-183 ◽  
Author(s):  
Duell E. Wood ◽  
Alan C. Pipkin
Keyword(s):  
Cell Culture ◽  
Trypanosoma Cruzi ◽  
Insect Cell ◽  
Culture System ◽  
Insect Cell Culture ◽  
Cell Culture System

Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles

2007 ◽  
Vol 103 (6) ◽  
pp. 572-574 ◽  
Author(s):  
Takuya Shishido ◽  
Masaru Muraoka ◽  
Hideki Yamaji ◽  
Akihiko Kondo ◽  
Hideki Fukuda
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Insect Cell Culture ◽  
Biomass Support Particles

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

Multilayer cell culture system supported by thread

2018 ◽  
Vol 257 ◽  
pp. 650-657 ◽  
Author(s):  
Azadeh Nilghaz ◽  
Siew Hoo ◽  
Wei Shen ◽  
Xiaonan Lu ◽  
Peggy P.Y. Chan
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Cell Culture System

Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS)

2015 ◽  
Vol 120 ◽  
pp. 279-285 ◽  
Author(s):  
Sabine Schnell ◽  
Kafilat Bawa-Allah ◽  
Adebayo Otitoloju ◽  
Christer Hogstrand ◽  
Thomas H. Miller ◽  
...  
Keyword(s):  
Cell Culture ◽  
Environmental Monitoring ◽  
Culture System ◽  
Urban Streams ◽  
Cell Culture System ◽  
Fish Gill ◽  
Gill Cell

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell
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