Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

10.3791/63008 ◽  
2021 ◽  
Author(s):  
Bianca B. Kojin ◽  
Hitoshi Tsujimoto ◽  
Emma Jakes ◽  
Sarah O'Leary ◽  
Zach N. Adelman
Keyword(s):  
High Resolution ◽  
Aedes Aegypti ◽  
High Resolution Melt ◽  
Indel Detection ◽  
High Resolution Melt Analysis

scholarly journals Rapid Determination of Lymphogranuloma Venereum Serovars of Chlamydia trachomatis by Quantitative High-Resolution Melt Analysis (HRMA)

2012 ◽  
Vol 50 (11) ◽  
pp. 3751-3753 ◽  
Author(s):  
J. Twin ◽  
M. P. Stevens ◽  
S. M. Garland ◽  
A. M. Zaia ◽  
S. N. Tabrizi
Keyword(s):  
High Resolution ◽  
Chlamydia Trachomatis ◽  
Rapid Determination ◽  
Lymphogranuloma Venereum ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

scholarly journals High-resolution melt analysis to detect sequence variations in highly homologous gene regions: application to CYP2B6

2013 ◽  
Vol 14 (8) ◽  
pp. 913-922 ◽  
Author(s):  
Greyson P Twist ◽  
Roger Gaedigk ◽  
J Steven Leeder ◽  
Andrea Gaedigk
Keyword(s):  
High Resolution ◽  
Homologous Gene ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Sequence Variations

A rapid method for sex identification in Cannabis sativa using High Resolution Melt (HRM) analysis.

Botany ◽  
2021 ◽  
Author(s):  
Erin Jacqueline Gilchrist ◽  
Daniela Hegebarth ◽  
Shumin Wang ◽  
Teagen D. Quilichini ◽  
Jason Sawler ◽  
...  
Keyword(s):  
High Resolution ◽  
Rapid Method ◽  
X Chromosome ◽  
Cannabis Sativa ◽  
Sex Identification ◽  
Male Plant ◽  
High Resolution Melt ◽  
Male And Female ◽  
High Resolution Melt Analysis ◽  
Plant Sex

We report the identification of two SNPs in Cannabis sativa that are associated with female and male plant sex phenotypes, and are located on the top arm of the X chromosome. High Resolution Melt analysis was used to develop and validate a novel, rapid method for sex identification in medical/recreational cannabis as well as in hemp. This method can distinguish between dioecious male (XY) and dioecious female (XX) cannabis plants with 100% accuracy, and can also be used to differentiate between male and female Humulus lupulus (hop) plants.

High-Resolution Melt Analysis

2013 ◽  
pp. 409-422 ◽  
Author(s):  
Einar Berg ◽  
Tania Nolan
Keyword(s):  
High Resolution ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

scholarly journals Detecting and Differentiating Phytoplasmas Belonging to Subgroups 16SrIV-A and 16SrIV-D Associated With Lethal Declines of Palms in Florida Using qPCR and High-Resolution Melt Analysis (HRMA)

Plant Disease ◽  
2017 ◽  
Vol 101 (8) ◽  
pp. 1449-1454 ◽  
Author(s):  
Brian W. Bahder ◽  
Ericka E. Helmick ◽  
Nigel A. Harrison
Keyword(s):  
High Resolution ◽  
Sequence Data ◽  
Management Strategies ◽  
Economic Losses ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Melting Temperatures ◽  
Detection And Identification ◽  
Lethal Yellowing ◽  
Sufficient Variation

Lethal yellowing (LY) and Texas Phoenix palm decline (TPPD) are two important phytoplasma diseases of palms in Florida. Both have been responsible for major economic losses historically and remain a constant threat to the sustainability of palm production in the landscaping and nursery industries in Florida. These two diseases cause rapid, lethal decline in afflicted palms, so rapid detection and identification is crucial to implement appropriate management strategies to reduce further spread and losses. In this study, a qPCR assay was developed to detect and identify the causal agents of LY and TPPD. Based on sequence data of the 16S gene for the 16SrIV-A phytoplasma (LY) and the 16SrIV-D phytoplasma (TPPD), two regions were identified in the gene that possessed sufficient variation to yield amplicons with measurable differences in melting temperature based on high resolution melt analysis (HRMA). One region was in the 5′ region and the other was located in the 3′ region of the gene. Products from both regions yielded amplicons with significantly different melting temperatures between the two phytoplasma strains. This research allows for the detection and identification of phytoplasmas in palms rapidly by eliminating many lengthy and post-PCR steps commonly used in phytoplasma identification.

Frequency of allotype “b” in human platelet antigen 1 to 29 systems among blood donors in Japan estimated using high‐resolution melt analysis

Transfusion ◽  
2020 ◽  
Vol 60 (11) ◽  
pp. 2702-2713
Author(s):  
Tomoya Hayashi ◽  
Ryota Aminaka ◽  
Hiroyuki Ishii ◽  
Yoshihiko Tani ◽  
Yoshihiro Fujimura ◽  
...  
Keyword(s):  
High Resolution ◽  
Blood Donors ◽  
Human Platelet ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Platelet Antigen ◽  
Human Platelet Antigen

Combining fine-needle aspiration biopsy (FNAB) and high-resolution melt analysis to reduce diagnostic delay in mycobacterial lymphadenitis

2009 ◽  
Vol 38 (7) ◽  
pp. 482-488 ◽  
Author(s):  
Colleen A. Wright ◽  
Kim G. P. Hoek ◽  
Ben J. Marais ◽  
Paul van Helden ◽  
Rob M. Warren
Keyword(s):  
High Resolution ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Diagnostic Delay ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
High Resolution Melt ◽  
Fine Needle ◽  
High Resolution Melt Analysis ◽  
Needle Aspiration Biopsy

Real-time PCR and high-resolution melt analysis methods for detection of pathogenic species of Brucella

2017 ◽  
Vol 41 (6) ◽  
Author(s):  
Faramarz Masjedian Jazi ◽  
Reza Mirnejad ◽  
Vahhab Piranfar ◽  
Noor Amir Mozafari ◽  
Taghi Zahraei Salehi ◽  
...  
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Serum Antibody ◽  
Clinical Samples ◽  
Species Differentiation ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Antibody Titers ◽  
Polymerase Chain ◽  
Animal Isolates

AbstractBackground:It is of great importance to quickly and accurately detectMethods:The current study describes a new method for the detection of brucellosis in clinical samples using real-time polymerase chain reaction (PCR) and high-resolution melt (HRM) curve analysis. This study was conducted on 70 human and 55 animal isolates with more than 1/80 serum antibody titers. Additionally, the accuracy and specificity of the methods were compared.Results:The mean range [cycles threshold±standard deviation (CConclusions:The results of HRM analysis can be used for species differentiation and bacterial genotyping according to nucleotide polymorphism. This molecular method could help in diagnosing

scholarly journals Rapid Identification and Discrimination ofBrucellaIsolates by Use of Real-Time PCR and High-Resolution Melt Analysis

2010 ◽  
Vol 48 (3) ◽  
pp. 697-702 ◽  
Author(s):  
Jonas M. Winchell ◽  
Bernard J. Wolff ◽  
Rebekah Tiller ◽  
Michael D. Bowen ◽  
Alex R. Hoffmaster
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Identification ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

scholarly journals Correction to: High-resolution melt analysis enables simple genotyping of complicated polymorphisms of codon 18 rendering the NUDT15 diplotype

2019 ◽  
Vol 55 (1) ◽  
pp. 132-132
Author(s):  
Yoichi Kakuta ◽  
◽  
Yasuhiro Izumiyama ◽  
Daisuke Okamoto ◽  
Takeru Nakano ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis
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