Employing Aeroponic Systems for the Clonal Propagation of Cannabis

10.3791/63117 ◽  
2021 ◽  
Author(s):  
Trevor Regas ◽  
Joon-Hee Han ◽  
Christopher S. Pauli ◽  
Sang-Hyuck Park
Keyword(s):  
Clonal Propagation

scholarly journals Whole genome resequencing and custom genotyping unveil clonal lineages in ‘Malbec’ grapevines (Vitis vinifera L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luciano Calderón ◽  
Nuria Mauri ◽  
Claudio Muñoz ◽  
Pablo Carbonell-Bejerano ◽  
Laura Bree ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Somatic Mutations ◽  
Clonal Propagation ◽  
Variant Calling ◽  
Vitis Vinifera L ◽  
Whole Genome ◽  
Single Nucleotide Variants ◽  
Genome Resequencing ◽  
Diversity Pattern ◽  
Whole Genome Resequencing

AbstractGrapevine cultivars are clonally propagated to preserve their varietal attributes. However, genetic variations accumulate due to the occurrence of somatic mutations. This process is anthropically influenced through plant transportation, clonal propagation and selection. Malbec is a cultivar that is well-appreciated for the elaboration of red wine. It originated in Southwestern France and was introduced in Argentina during the 1850s. In order to study the clonal genetic diversity of Malbec grapevines, we generated whole-genome resequencing data for four accessions with different clonal propagation records. A stringent variant calling procedure was established to identify reliable polymorphisms among the analyzed accessions. The latter procedure retrieved 941 single nucleotide variants (SNVs). A reduced set of the detected SNVs was corroborated through Sanger sequencing, and employed to custom-design a genotyping experiment. We successfully genotyped 214 Malbec accessions using 41 SNVs, and identified 14 genotypes that clustered in two genetically divergent clonal lineages. These lineages were associated with the time span of clonal propagation of the analyzed accessions in Argentina and Europe. Our results show the usefulness of this approach for the study of the scarce intra-cultivar genetic diversity in grapevines. We also provide evidence on how human actions might have driven the accumulation of different somatic mutations, ultimately shaping the Malbec genetic diversity pattern.

Nitrogen and litter addition decreased sexual reproduction and increased clonal propagation in grasslands

Oecologia ◽  
2021 ◽  
Vol 195 (1) ◽  
pp. 131-144
Author(s):  
Zimeng Li ◽  
Jinfeng Wu ◽  
Qing Han ◽  
Kunyan Nie ◽  
Jiani Xie ◽  
...  
Keyword(s):  
Sexual Reproduction ◽  
Clonal Propagation ◽  
Litter Addition

Influence of some adjuvants on in vitro clonal propagation of male and female jojoba plants

2003 ◽  
Vol 39 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Surya Prakash ◽  
Veena Agrawal ◽  
S. C. Gupta
Keyword(s):  
Clonal Propagation ◽  
Male And Female ◽  
In Vitro Clonal Propagation

scholarly journals Influence of Cytokinins in Combination with GA3on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis(L.) O. Kuntze)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Reza Azadi Gonbad ◽  
Uma Rani Sinniah ◽  
Maheran Abdul Aziz ◽  
Rosfarizan Mohamad
Keyword(s):  
Camellia Sinensis ◽  
Woody Plants ◽  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Liquid Form ◽  
Solid Media

The use ofin vitroculture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis(L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA3) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA3. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

Rapid clonal propagation of rhubarb by in vitro culture of shoot-tips

1979 ◽  
Vol 11 (3) ◽  
pp. 241-246 ◽  
Author(s):  
Joseph Roggemans ◽  
Marie-Christine Claes
Keyword(s):  
In Vitro Culture ◽  
Clonal Propagation ◽  
Shoot Tips

Designing food and habitat trees for urban koalas: Tree height, foliage palatability and clonal propagation of Eucalyptus kabiana

2017 ◽  
Vol 27 ◽  
pp. 196-202 ◽  
Author(s):  
Stephen J. Trueman ◽  
Tracey V. McMahon ◽  
Elektra L. Grant ◽  
David A. Walton ◽  
Peter H. Theilemann ◽  
...  
Keyword(s):  
Clonal Propagation ◽  
Tree Height

Derivation of a New Human Embryonic Stem Cell Line, Endeavour-1, and Its Clonal Propagation

2008 ◽  
Vol 17 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Kuldip S. Sidhu ◽  
John P. Ryan ◽  
Bernie E. Tuch
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Line ◽  
Human Embryonic Stem Cell ◽  
Clonal Propagation ◽  
Embryonic Stem Cell Line ◽  
Embryonic Stem ◽  
Stem Cell Line

Hormonal effects on clonal propagation of Wedelia chinensis L.

2008 ◽  
Vol 136 ◽  
pp. S162
Author(s):  
Md. Asraful Alam ◽  
Tamanna A. Jahan ◽  
Fazlima Parveen ◽  
Mujib Khan ◽  
Rownak Jahan ◽  
...  
Keyword(s):  
Clonal Propagation ◽  
Hormonal Effects

Clonal Propagation of Chilopsis Linearis (CAV.) Through Tissue Culture

1985 ◽  
pp. 354-355
Author(s):  
David W. Still ◽  
John F. Hubstenberger ◽  
Gregory C. Phillips ◽  
Ronald F. Hooks
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Chilopsis Linearis
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