Peripheral Blood c-erbB-2 Expression by Reverse Transcriptase—Polymerase Chain Reaction in Breast Cancer Patients Receiving Chemotherapy

2002 ◽  
Vol 3 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Fernando L.A. Fonseca ◽  
Heloisa P. Soares ◽  
Ana Rita Manhani ◽  
Israel Bendit ◽  
Mafalda Novaes ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Breast Cancer Patients ◽  
Chain Reaction ◽  
Polymerase Chain

Predictive and Prognostic Value of Peripheral Blood Cytokeratin-19 mRNA-Positive Cells Detected by Real-Time Polymerase Chain Reaction in Node-Negative Breast Cancer Patients

2006 ◽  
Vol 24 (23) ◽  
pp. 3756-3762 ◽  
Author(s):  
Nikos Xenidis ◽  
Maria Perraki ◽  
Maria Kafousi ◽  
Stella Apostolaki ◽  
Irene Bolonaki ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Cytokeratin 19 ◽  
Breast Cancer Patients ◽  
Chain Reaction ◽  
Node Negative ◽  
Node Negative Breast Cancer ◽  
Polymerase Chain ◽  
Predictive And Prognostic Value

PurposeTo evaluate the predictive and prognostic value of peripheral blood cytokeratin-19 (CK-19) mRNA-positive cells in axillary lymph node–negative breast cancer patients.Patients and MethodsPeripheral blood was obtained from 167 node-negative breast cancer patients before the initiation of any systemic adjuvant therapy, and was analyzed for the presence of CK-19 mRNA-positive cells using a real time polymerase chain reaction assay. The association with known prognostic factors and the effect of CK-19 mRNA-positive cells on patients' prognosis was investigated.ResultsCK-19 mRNA-positive cells were detected in the blood of 36 (21.6%) of the 167 patients. There was no correlation between the detection of CK-19 mRNA-positive cells in the peripheral blood and the various known pathologic and clinical prognostic factors; only overexpression of HER2 receptor (score 2+/3+) on the primary tumor was associated with a higher incidence of CK-19 mRNA-positive cell detection (P = .033). Multivariate analysis revealed that detection of peripheral blood CK-19 mRNA-positive cells was associated with early clinical relapse (P < .00001) and disease-related death (P = .008).ConclusionDetection of peripheral-blood CK-19 mRNA-positive cells is an independent predictive and prognostic factor for reduced disease-free interval and overall survival, respectively, in node-negative breast cancer patients.

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