Using direct epifluorescent microscopic count for rapid enumeration of viable yeast and bacteria in injured conditions

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
C.-C. Huang ◽  
S.-M. Liau ◽  
W.-C. Tsai ◽  
H.-H. Wang
Keyword(s):  
Rapid Enumeration ◽  
Microscopic Count

Pyruvate as an Indicator of Quality in Grading Nonfat Dry Milk1

1982 ◽  
Vol 45 (6) ◽  
pp. 561-565 ◽  
Author(s):  
R. T. MARSHALL ◽  
Y. H. LEE ◽  
B. L. O'BRIEN ◽  
W. A. MOATS
Keyword(s):  
Geometric Mean ◽  
Regression Line ◽  
Skim Milk ◽  
Plate Count ◽  
Department Of Agriculture ◽  
Standard Plate Count ◽  
Geometric Average ◽  
Dry Milk ◽  
Standard Plate ◽  
Microscopic Count

Samples of skim milk and nonfat dry milk (NDM) made from it were collected, paired and tested for pyruvate concentration, [P], and Direct Microscopic count (DMC). The skim milk was tested for Standard Plate Count (SPC) and Psychrotrophic Plate Count (PPC). The geometric average DMC of skim milk was more than three times higher than that of the paired NDM samples. However, [P] of NDM was not significantly different from that of the skim milk. Although [P] of skim milk was poorly correlated with SPC and PPC, r = .31 and .26, respectively, it was relatively well correlated with DMC, r = .64. Data were widely dispersed around the regression line when [P] was ≤ 4.0 mg/L. However, [P] increased rapidly when DMCs were > 106/ml. A limit of 10 mg/L of [P] in NDM reconstituted 1:9 was chosen to represent the current U.S. Department of Agriculture Standard for DMC in NDM. This limit failed to classify about 10% of the samples correctly, assuming that each geometric mean DMC was correct. However, the probability that samples meeting the DMC standard would be rejected by the pyruvate test was quite low and the probability was moderate that samples which would be acceptable by the pyruvate test would be rejected by the DMC. For the latter, 28% of the samples having DMCs of ≥ 107/ml contained < 10 mg/L of pyruvate. No sample having ≥ 10 mg/L of pyruvate had a DMC of ≤ 107/ml. Pyruvate concentration in NDM did not change during storage at 5 or 32°C for 90 days.

Interactive algorithms for rapid enumeration of hybridization signals in individual whole-cell nuclei inside intact-tissue specimens

1995 ◽  
Author(s):  
Stephen J. Lockett ◽  
Curtis T. Thompson ◽  
James C. Mullikin ◽  
Damir Sudar ◽  
R. Khavari ◽  
...  
Keyword(s):  
Cell Nuclei ◽  
Whole Cell ◽  
Intact Tissue ◽  
Rapid Enumeration ◽  
Tissue Specimens ◽  
Interactive Algorithms

scholarly journals PIECES FRAMEWORK ON THE IMPLEMENTATION OF RAPID ENUMERATION AND EVALUATION INFORMATION SYSTEM OF 2020 POPULATION CENSUS

2021 ◽  
Vol 17 (1) ◽  
pp. 47-52
Author(s):  
Rizka Ita Yuanita
Keyword(s):  
Information System ◽  
User Satisfaction ◽  
Population Census ◽  
Analysis Model ◽  
Framework Analysis ◽  
West Java ◽  
Major Activity ◽  
Rapid Enumeration ◽  
Environmental Unit ◽  
Evaluation Information

In September 2020, Statistics Indonesia (BPS RI) carried out a major activity, namely the 2020 Population Census At the SP2020 data processing stage, Mobile Capture is one of the options for photographing data collection results. During the use of mobile capture, BPS West Java Province encountered several obstacles. West Java Province BPS created an application called the Rapid Enumeration dan Evaluation 2020 Population Census Information System (Sicepat32 SLS) to monitor the attainment of enumeration to the smallest level (local environmental unit). To find out whether Sicepat32 SLS is working and functioning properly, it is necessary to evaluate the performance of the information system. The purpose of this study is to measure the level of user satisfaction with the application of Sicepat32 SLS and to assess whether Sicepat32 SLS can meet the needs of users. In this study, the PIECES Framework analysis model will be used and has produced an assessment score in the Performance domain of 4.07; Information and Data of 4.17; Economy 4.1; Control and Security with a score of 4.03; Efficiency with a score of 4.18 and Service getting a score of 4.18

Digoxigenin-Labeled Probe-Based Colony Blotting Assay for Rapid Quantification of Salmonella Serovars in Seafood and Water

2012 ◽  
Vol 95 (6) ◽  
pp. 1652-1655 ◽  
Author(s):  
Rakesh Kumar ◽  
K V Lalitha
Keyword(s):  
Real Time ◽  
Water Samples ◽  
Real Time Pcr ◽  
Quantitative Detection ◽  
Gene Probe ◽  
Pcr Assay ◽  
Nylon Membrane ◽  
Rapid Enumeration ◽  
Salmonella Serovars ◽  
Labeled Probe

Abstract A non-radio-labeled probe-based detection method was developed for rapid enumeration of Salmonella in seafood and water samples. A Salmonella-specific invA gene probe was developed using a digoxigenin-based non-radio labeling assay, which was evaluated with naturally contaminated seafood and water samples. The probe-based technique was further compared with the quantitative PCR assay. The method was specific for detection of different Salmonella serovars without any nonspecific hybridization with other Salmonella-related Enterobacteriaceae. The optimum labeling efficiency was determined for the labeled probe, and 10 pg/μL probe concentration was observed to be most efficient for detection of Salmonella colonies on nylon membrane. Quantification of Salmonella in naturally contaminated seafood and water samples (n = 21) was in the range 10–102 CFU/mL. The assay successfully quantified Salmonella in spiked seafood and water samples in the presence of background flora, and the entire assay was completed within 48 h. The probe-based assay was further evaluated with real-time PCR, and results showed that the assay was comparable to real-time PCR assay. Thus, this probe-based assay can be a rapid, useful, and alternative technique for quantitative detection of Salmonella in food, feed, and water samples.

Rapid enumeration of respiratory active mycobacteria with fluorescent double staining

2010 ◽  
Vol 82 (3) ◽  
pp. 327-329 ◽  
Author(s):  
Tomoaki Ichijo ◽  
Yoko Izumi ◽  
Nobuyasu Yamaguchi ◽  
Masao Nasu
Keyword(s):  
Double Staining ◽  
Rapid Enumeration

Rapid Detection of Psychrotrophic Bacteria In Manufacturing Grade Raw Milks

1991 ◽  
Vol 54 (11) ◽  
pp. 861-867 ◽  
Author(s):  
S. R. TATINI ◽  
P. MEKALA ◽  
A. EL-HABAZ ◽  
M. W. GRIFFITHS
Keyword(s):  
Rapid Detection ◽  
Bacterial Count ◽  
Raw Milk ◽  
Plate Count ◽  
Psychrotrophic Bacteria ◽  
Milk Samples ◽  
Microscopic Count ◽  
Direct Epifluorescent Filter Technique ◽  
Native Microflora

Methods to rapidly assess the bacteriological quality of raw milk were investigated. Whereas direct microscopic count, modified psychrotrophic plate count, and direct epifluorescent filter technique (DEFT) did not correlate well with initial psychrotrophic bacterial count of raw milk, improvements were obtained after preincubation of the milk samples. The best preincubation conditions were identified as 30°C for 6 h, 21°C for 10 h, 13°C for 15 h, 13°C for 20 h, or 7°C for 37 h. The “square root” equation was applied to the data, and a model was produced for predicting growth of the native microflora of raw milk. Using this equation, a DEFT count after preincubation of the milk at 21°C for 10 h could accurately predict the initial psychrotroph count and the count after storage of the milk at 6°C for 48 h.

The Validation of the MicroSnap Total for Enumeration of Total Viable Count in a Variety of Foods

2016 ◽  
Vol 99 (3) ◽  
pp. 686-694
Author(s):  
Paul Meighan ◽  
Mat Smith ◽  
Shreya Datta ◽  
Brandon Katz ◽  
Fred Nason
Keyword(s):  
Adenosine Triphosphate ◽  
Growth Phase ◽  
Ground Beef ◽  
Raw Milk ◽  
Viable Count ◽  
Light Signal ◽  
Total Viable Count ◽  
Rapid Enumeration ◽  
Working Shift ◽  
Quantitative Results

Abstract Hygiena's MicroSnap™ Total enables rapid enumeration of total viable count (TVC) within an 8 h working shift. The method measures the biomarker adenosine triphosphate (ATP), which develops a light signal proportional to the concentration of biomarker discovered. The two-step assay determines ATP concentration and hence the concentration of bacteria in the sample at the onset of the growth phase. In the first phase, the sample is enriched in a broth containing ATP-depleting enzymes that reduce background ATP from sample matrixes. After the 7 h enrichment phase, an aliquot is taken from the Enrichment Device using a built-in pipet and transferred into a secondary Detection Device. The Detection Device is then activated and measured as relative light units in a Hygiena luminometer for quantitative results in just 15 s. This study measures and compares the natural TVC from five sample matrixes: raw ground beef, raw milk, lettuce, raw chicken, and freshly produced cream cake. MicroSnap Total successfully enumerates TVC from all samples over an average range of log 2.00–6.50; equivalence is demonstrated by an overall r2 value of 0.9184.

Whole slide imaging of circulating tumor cells captured on a capillary microchannel device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3796-3803 ◽  
Author(s):  
Yiting Zheng ◽  
Qingxuan Li ◽  
Weike Hu ◽  
Jun Liao ◽  
Guoan Zheng ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Polymer Brushes ◽  
Blood Sampling ◽  
Whole Slide Imaging ◽  
Rapid Enumeration ◽  
Microchannel Device

The integrated capillary microchannel modified with polymer brushes allows direct blood sampling, efficient CTC capturing, and rapid enumeration with whole slide imaging.

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