Response Surface Optimized Ultrasonic Assisted Extraction of Total Flavonoids from Walnut Leaves and In Vitro Antibacterial Activities

The Box-Behnken design combined with response surface method was employed to optimize ultrasonic-assisted extraction of flavonoids fromTaraxacum officinale. The optimized results showed that the highest extraction yield with ultrasonic-assisted extraction could reach 2.62% using 39.6% (v/v) ethanol and 59.5 : 1 (mL/g) liquid-solid ratio for 43.8 min. The crude extract was then purified by HPD-100 macroporous adsorption resin, and the flavonoids content in the purified extract increased to 54.7%. The antioxidant activity of the purified flavonoids was evaluatedin vitroby scavenging capacity of ABTS or DPPH,β-carotene bleaching, and FTC test. The knowledge obtained from this study should be useful to further develop and apply this plant resource.

Download Full-text

Response Surface Optimized Ultrasonic Assisted Extraction of Total Flavonoids from QingLi Cao and Antioxidant Activities

The Open Biotechnology Journal ◽

10.2174/1874070701509010134 ◽

2015 ◽

Vol 9 (1) ◽

pp. 134-142 ◽

Cited By ~ 2

Author(s):

Xudong Jiang ◽

YaoLing Liao ◽

GuiXi Lu ◽

Zhike Xiao

Keyword(s):

Response Surface ◽

Antioxidant Activities ◽

Reducing Power ◽

Total Flavonoids ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

An ultrasound-assisted extraction technique was used to extract the total flavonoids from QingLi Cao. The optimal conditions were ethanol concentration 59.20%, liquid-to-solid ratio 31.15 mL/g, extraction time 57.42 min and extraction temperature 58.57°C, which were determined using response surface methodology. The antioxidant activities including reducing power, ABTS+, DPPH, superoxide anion and hydroxyl radical were evaluated, which suggested significant antioxidant activities.

Download Full-text

Optimizing of Ultrasonic-Assisted Extraction Technology of Total Flavonoids from Bambusae caulis in Taenias Based on Response Surface Methodology

Pharmacy Information ◽

10.12677/pi.2017.63011 ◽

2017 ◽

Vol 06 (03) ◽

pp. 63-71

Author(s):

红梅管

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Total Flavonoids ◽

Extraction Technology ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

Download Full-text

Optimized Ultrasonic-Assisted Extraction by Using Response Surface Methodology and Structural Analysis of Flavonoids from Alliums cepa L. var. agrogatum Don

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.634-638.1274 ◽

2013 ◽

Vol 634-638 ◽

pp. 1274-1280

Author(s):

Hai Yue Zhang ◽

Peng Li

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

High Performance ◽

Extraction Yield ◽

Uv Spectrophotometry ◽

Total Flavonoids ◽

Resin Column ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

The response surface methodology (RSM) was used to optimize ultrasonic-assisted extraction of total flavonoids from Alliums cepa L. var. agrogatum Don (ACAD). The results indicated that the highest extraction yield of flavonoids by ultrasonic-assisted extraction could obtain to be 3.36% using ethanol concentration of 70% (v/v) as solvent and liquid to solid ratio of 17:1 (mL/g) for 48 min at the temperature of 60 °C. The total flavonoids were purified by AB-8 resin column and polyamide resin column successively. In addition, the maximum absorption wavelength of ACAD flavonoids was determined by UV spectrophotometry, followed by their structural identified and analysis for flavonoids by infrared spectroscopy, high performance liquid chromatography. It could be concluded that the flavonoids of ACAD contains quercetin.

Download Full-text