scholarly journals In Situ Observation of the Self-Assembling of Oxides Nanoparticles in Water by Electron Microscopy

2019 ◽  
Vol 35 (5) ◽  
pp. 459-460
Author(s):  
Kai WU ◽  
2020 ◽  
Vol 27 (9) ◽  
pp. 923-929
Author(s):  
Gaurav Pandey ◽  
Prem Prakash Das ◽  
Vibin Ramakrishnan

Background: RADA-4 (Ac-RADARADARADARADA-NH2) is the most extensively studied and marketed self-assembling peptide, forming hydrogel, used to create defined threedimensional microenvironments for cell culture applications. Objectives: In this work, we use various biophysical techniques to investigate the length dependency of RADA aggregation and assembly. Methods: We synthesized a series of RADA-N peptides, N ranging from 1 to 4, resulting in four peptides having 4, 8, 12, and 16 amino acids in their sequence. Through a combination of various biophysical methods including thioflavin T fluorescence assay, static right angle light scattering assay, Dynamic Light Scattering (DLS), electron microscopy, CD, and IR spectroscopy, we have examined the role of chain-length on the self-assembly of RADA peptide. Results: Our observations show that the aggregation of ionic, charge-complementary RADA motifcontaining peptides is length-dependent, with N less than 3 are not forming spontaneous selfassemblies. Conclusion: The six biophysical experiments discussed in this paper validate the significance of chain-length on the epitaxial growth of RADA peptide self-assembly.


2014 ◽  
Vol 70 (3) ◽  
pp. 326-331 ◽  
Author(s):  
Estelle Mossou ◽  
Susana C. M. Teixeira ◽  
Edward P. Mitchell ◽  
Sax A. Mason ◽  
Lihi Adler-Abramovich ◽  
...  

The title zwitterion (2S)-2-azaniumyl-1-hydroxy-3-phenylpropan-1-olate, C9H11NO2, also known as L-phenylalanine, was characterized using synchrotron X-rays. It crystallized in the monoclinic space groupP21with four molecules in the asymmetric unit. The 0.62 Å resolution structure is assumed to be closely related to the fibrillar form of phenylalanine, as observed by electron microscopy and electron diffraction. The structure exists in a zwitterionic form in which π–π stacking and hydrogen-bonding interactions are believed to form the basis of the self-assembling properties.


Nanoscale ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 34-44 ◽  
Author(s):  
Eli Sutter ◽  
Bo Zhang ◽  
Stephan Sutter ◽  
Peter Sutter

In situ liquid cell electron microscopy of the pH-driven assembly of single stranded DNA-functionalized Au nanoparticles in aqueous solution.


Soft Matter ◽  
2007 ◽  
Vol 3 (7) ◽  
pp. 872 ◽  
Author(s):  
Masahiko Ishii ◽  
Masashi Harada ◽  
Hiroshi Nakamura

2000 ◽  
Vol 162-163 ◽  
pp. 384-389 ◽  
Author(s):  
T Stimpel ◽  
J Schulze ◽  
H.E Hoster ◽  
I Eisele ◽  
H Baumgärtner

Nanoscale ◽  
2018 ◽  
Vol 10 (26) ◽  
pp. 12674-12682 ◽  
Author(s):  
Peter Sutter ◽  
Bo Zhang ◽  
Eli Sutter

In situ electron microscopy in liquids is used to establish radiation damage pathways and damage-free imaging conditions for superlattices of oligonucleotide–nanoparticle conjugates, paving the way for imaging the self-assembly of such programmable atom equivalents in aqueous solution.


2007 ◽  
Vol 1057 ◽  
Author(s):  
Grigory Tikhomirov ◽  
Hicham Fenniri

ABSTRACTThe self-assembly of six self-complimentary Guanine – Cytosine hybrid heterocycles bearing hydrophobic substituents has been studied using combinatorial approach in eight solvents under different conditions. The parameters that were varied include: the structure of the self-assembling module, its concentration, the solvent, temperature, and time of self-assembly. scanning electron microscopy (SEM) was used as a screening tool. A wide variety of interesting morphologies was found. The most interesting structures were studied by atomic force microscopy (AFM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and X-ray powder diffraction (XRD).


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